In:
Plant Genetic Resources, Cambridge University Press (CUP), Vol. 12, No. S1 ( 2014-07), p. S151-S154
Abstract:
In this study, a near-isogenic line (BC 4 F 10 ) CR572 developed by introgressing a chromosomal segment from Oryza rufipogon (accession no. 105491) into the Oryza sativa subsp. japonica cv. Hwaseong was found to exhibit a significant increase in the number of spikelets per panicle (SPP) and grain weight compared with the recurrent parent Hwaseong. Quantitative trait locus (QTL) analysis in F 2 generation derived from the cross between CR572 and Hwaseong revealed that two QTLs, qSPP1 and qTGW1 , were linked to a simple sequence repeat marker, RM283, on chromosome 1. The additive effect of the O. rufipogon allele at qSPP1 was 13 SPP, and 21.6% of the phenotypic variance was explained by the segregation of RM283. The qTGW1 QTL explained 19.1% of the phenotypic variance for grain weight. Substitution mapping was carried out with five F 3 lines derived from F 2 plants having informative recombination breakpoints within the target region. Substitution mapping indicated the linkage of qSPP1 and qTGW1 . The grain yield of CR572 was 18.2 and 15.8% higher than that of Hwaseong at two locations, respectively, mainly due to the increase in 1000-grain weight and SPP. These results are very useful for QTL cluster transfer by molecular marker-assisted selection in rice breeding programmes and for QTL gene cloning by map-based cloning.
Type of Medium:
Online Resource
ISSN:
1479-2621
,
1479-263X
DOI:
10.1017/S1479262114000562
Language:
English
Publisher:
Cambridge University Press (CUP)
Publication Date:
2014
detail.hit.zdb_id:
2180556-8
SSG:
12
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