In:
Molecular Nutrition & Food Research, Wiley, Vol. 58, No. 3 ( 2014-03), p. 528-536
Abstract:
The cathelicidin antimicrobial peptide ( CAMP ) gene is induced by 1α,25‐dihydroxyvitamin D 3 (1α,25( OH ) 2 D 3 ), lithocholic acid, curcumin, nicotinamide, and butyrate. Discovering additional small molecules that regulate its expression will identify new molecular mechanisms involved in CAMP regulation and increase understanding of how diet and nutrition can improve immune function. Methods and results We discovered that two stilbenoids, resveratrol and pterostilbene, induced CAMP promoter‐luciferase expression. Synergistic activation was observed when either stilbenoid was combined with 1α,25( OH ) 2 D 3 . Both stilbenoids increased CAMP m RNA and protein levels in the monocyte cell line U 937 and synergy was observed in both U 937 and the keratinocyte cell line, H a C a T . Inhibition of resveratrol targets sirtuin‐1, cyclic AMP production and the c‐ J un N ‐terminal, phosphoinositide 3 and AMP ‐activated kinases did not block induction of CAMP by resveratrol or synergy with 1α,25( OH ) 2 D 3 . Nevertheless, inhibition of the extracellular signal regulated 1/2 and p38 mitogen‐activated protein kinases, increased CAMP gene expression in combination with 1α,25( OH ) 2 D 3 suggesting that inhibition of these kinases by resveratrol may explain, in part, its synergy with vitamin D . Conclusion Our findings demonstrate for the first time that stilbenoid compounds may have the potential to boost the innate immune response by increasing CAMP gene expression, particularly in combination with 1α,25( OH ) 2 D 3 .
Type of Medium:
Online Resource
ISSN:
1613-4125
,
1613-4133
DOI:
10.1002/mnfr.201300266
Language:
English
Publisher:
Wiley
Publication Date:
2014
detail.hit.zdb_id:
2160372-8
SSG:
12
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