In:
Journal of Materials Chemistry B, Royal Society of Chemistry (RSC), Vol. 11, No. 20 ( 2023), p. 4523-4528
Abstract:
Syphilis, caused by Treponema pallidum ( T. pallidum ), is associated with the oxidative stress due to its inflammation-like symptom, and detecting the reactive oxygen species (ROS) is crucial for monitoring the infectious process. Herein, we design and synthesize a perylene-based tunable fluorescent probe, PerqdOH, which can detect endogenous O 2 ˙ − during T. pallidum infection. The fluorescence peak shifted from 540 nm to 750 nm with increasing O 2 ˙ − levels. Besides, both decreased green fluorescence and enhanced red fluorescence could be observed simultaneously during the in vitro infection, providing the real-time monitoring of intracellular O 2 ˙ − caused by T. pallidum . Furthermore, the probe exhibited a remarkable signal in the treponemal lesions on the back of a rabbit model. Taken together, our synthesized PerqdOH holds great potential for application in clarifying the infectious process caused by T. pallidum in real time.
Type of Medium:
Online Resource
ISSN:
2050-750X
,
2050-7518
Language:
English
Publisher:
Royal Society of Chemistry (RSC)
Publication Date:
2023
detail.hit.zdb_id:
2702241-9
detail.hit.zdb_id:
2705149-3
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