In:
PROTEOMICS – Clinical Applications, Wiley, Vol. 1, No. 2 ( 2007-02), p. 215-223
Abstract:
To identify proteins associated with esophageal carcinogenesis, we performed protein profiling of 16 esophageal squamous cell carcinomas (ESCCs) and paired noncancerous tissues by 2‐DE and MS/MS. In cancerous tissues, three spots showed significant up‐regulation in the amount of protein, while eight spots were significantly down‐regulated. The identities of the spots were determined by PMF with LC‐MS/MS and were confirmed by immunoblotting. The up‐regulated proteins were tropomyosin alpha 4 chain, transgelin, and pyruvate kinase. The down‐regulated proteins were serum albumin precursor, isoforms of annexin A1, tropomyosin beta chain, 14‐3‐3 protein sigma, and isoforms of serotransferrin precursor. In all 16 cases, up‐regulation of the tropomyosin alpha 4 chain was confirmed by immunoblotting. Localization of the tropomyosin alpha 4 chain in ESCC cells and adjacent fibroblasts was confirmed by immunohistochemistry.
Type of Medium:
Online Resource
ISSN:
1862-8346
,
1862-8354
DOI:
10.1002/prca.200600609
Language:
English
Publisher:
Wiley
Publication Date:
2007
detail.hit.zdb_id:
2261788-7
detail.hit.zdb_id:
2317130-3
Permalink