In:
Cancer Research, American Association for Cancer Research (AACR), Vol. 70, No. 8_Supplement ( 2010-04-15), p. 2969-2969
Abstract:
ST7 has been proposed as a tumor suppressor gene at the chromosome region 7q31.1-q31.2. In order to gain some insight into its role in cancer, localization and verification of ST7 expression levels were performed. Various types of ST7 expression vectors tagged with sequences of GFP, YFP or V5 were created using gateway cloning system and full length ST7 cDNA isolated from a human adult brain cDNA library. Cytosolic ST7 expression in HCT-116, MCF-7 and PC-3 cell lines was detected via the fluorescence signal of the fusion proteins. ST7 translocation from cytoplasm to nucleus has not been observed in any of the conditions assayed. A cell cycle synchronization study demonstrated that both ST7 and SERPINE1 were over expressed when cells were arrested. Expression of these genes was found to be substantially diminished when the cells re-entered cell division status. In addition, we also found that Survivin, MMP13, and CyclinD1 were differentially expressed during cell cycle. Our findings suggest that ST7 mediates tumor suppression through regulation of the genes involved in maintaining cellular structure of the cell and involved in oncogenic pathways. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 2969.
Type of Medium:
Online Resource
ISSN:
0008-5472
,
1538-7445
DOI:
10.1158/1538-7445.AM10-2969
Language:
English
Publisher:
American Association for Cancer Research (AACR)
Publication Date:
2010
detail.hit.zdb_id:
2036785-5
detail.hit.zdb_id:
1432-1
detail.hit.zdb_id:
410466-3
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