In:
Macromolecular Bioscience, Wiley, Vol. 21, No. 11 ( 2021-11)
Abstract:
Cryogels are gel networks or scaffolds with a large porous structure; they can be tailored for injectability and for possessing a shape‐memory ability. Herein, a growth factor‐releasing cryogel microparticle (CMP) system is fabricated, and the therapeutic efficacy of recombinant human vascular endothelial growth factor (rhVEGF)‐loaded CMP (V‐CMP) for neovascularization is investigated. To prepare the cryogels, both methacrylated chitosan (Chi‐MA) and methacrylated chondroitin sulfate (CS‐MA) are used, and crosslinking using a radical crosslinking reaction is established. The physical, mechanical, and biological properties of the cryogels are analyzed by varying the amount of CS‐MA used. The cryogels are then pulverized, and microsized CMPs are fabricated. CMPs dispersed in saline demonstrate a shear‐thinning property, and can thus be extruded through a 23G needle. Additionally, V‐CMP exhibit a sustained release profile of rhVEGF and enhance the in vitro proliferation of endothelial cells. Finally, neovascularization and effective tissue necrosis prevention are observed when V‐CMPs are injected into a hindlimb ischemia mouse model. Thus, the injectable V‐CMP system developed herein demonstrates a high potential utility in various tissue regeneration applications based on cell or growth factor delivery.
Type of Medium:
Online Resource
ISSN:
1616-5187
,
1616-5195
DOI:
10.1002/mabi.202100234
Language:
English
Publisher:
Wiley
Publication Date:
2021
detail.hit.zdb_id:
2039130-4
SSG:
12
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