In:
Magnetic Resonance Materials in Physics, Biology and Medicine, Springer Science and Business Media LLC, Vol. 34, No. 1 ( 2021-02), p. 49-56
Abstract:
To enhance detection of the products of hyperpolarized [2- 13 C] dihydroxyacetone metabolism for assessment of three metabolic pathways in the liver in vivo. Hyperpolarized [2- 13 C]DHAc emerged as a promising substrate to follow gluconeogenesis, glycolysis and the glycerol pathways. However, the use of [2- 13 C]DHAc in vivo has not taken off because (i) the chemical shift range of [2- 13 C]DHAc and its metabolic products span over 144 ppm, and (ii) 1 H decoupling is required to increase spectral resolution and sensitivity. While these issues are trivial for high-field vertical-bore NMR spectrometers, horizontal-bore small-animal MR scanners are seldom equipped for such experiments. Methods Real-time hepatic metabolism of three fed mice was probed by 1 H-decoupled 13 C-MR following injection of hyperpolarized [2- 13 C]DHAc. The spectra of [2- 13 C]DHAc and its metabolic products were acquired in a 7 T small-animal MR scanner using three purpose-designed spectral-spatial radiofrequency pulses that excited a spatial bandwidth of 8 mm with varying spectral bandwidths and central frequencies (chemical shifts). Results The metabolic products detected in vivo include glycerol 3-phosphate, glycerol, phosphoenolpyruvate, lactate, alanine, glyceraldehyde 3-phosphate and glucose 6-phosphate. The metabolite-to-substrate ratios were comparable to those reported previously in perfused liver. Discussion Three metabolic pathways can be probed simultaneously in the mouse liver in vivo, in real time, using hyperpolarized DHAc.
Type of Medium:
Online Resource
ISSN:
0968-5243
,
1352-8661
DOI:
10.1007/s10334-020-00884-y
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2021
detail.hit.zdb_id:
1502491-X
SSG:
11
Permalink