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  • 11
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    Online Resource
    Investigation of effect of fullerenol on viscoelasticity properties of human hepatocellular carcinoma by AFM-based creep tests
    Springer Science and Business Media LLC ; 2017
    In:  Journal of Materials Research Vol. 32, No. 13 ( 2017-07), p. 2521-2531
    Details
    In: Journal of Materials Research, Springer Science and Business Media LLC, Vol. 32, No. 13 ( 2017-07), p. 2521-2531
    Abstract: Cellular elasticity is frequently measured to investigate the biomechanical effects of drug treatment, diseases, and aging. In light of the cellular viscosity property exhibited by filament actin networks, this study investigates the viscoelasticity alterations of the human hepatocellular carcinoma (SMMC-7721) cell subjected to fullerenol treatment by means of creep tests realized by atomic force microscopy indentation. An SMMC-7721 cell was first modeled as a sphere and then as a flattened layer with finite thickness. Both Sneddon’s solutions and the Dimitriadis model have been modified to adapt to the viscoelastic situation, which are used to fit the same indentation depth–time curves obtained by creep tests. We find that the SMMC-7721 cell’s creep behavior is well described by the two modified models and the divergence of parameters determined by the two models is justified. By fullerenol treatment, the SMMC-7721 cell exhibits a significant decrease of elastic modulus and viscosity, which is presumably due to the disruption of actin filaments. This work represents a new attempt to understand the alternation of the viscoelastic properties of cancerous cells under the treatment of fullerenol, which has the significance of comprehensively elucidating the biomechanical effects of anticancer agents (such as fullerenol) on cancer cells.
    Type of Medium: Online Resource
    ISSN: 0884-2914 , 2044-5326
    URL: Article
    DOI: 10.1557/jmr.2017.229
    RVK:
    VA 5350
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2017
    detail.hit.zdb_id: 54876-5
    detail.hit.zdb_id: 2015297-8
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  • 12
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    Table_1.docx
    Frontiers Media SA ; 2020
    In:  Frontiers in Microbiology Vol. 11 ( 2020-9-25)
    Details
    In: Frontiers in Microbiology, Frontiers Media SA, Vol. 11 ( 2020-9-25)
    Type of Medium: Online Resource
    ISSN: 1664-302X
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    DOI: 10.3389/fmicb.2020.588099
    DOI: 10.3389/fmicb.2020.588099.s001
    DOI: 10.3389/fmicb.2020.588099.s002
    DOI: 10.3389/fmicb.2020.588099.s003
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2020
    detail.hit.zdb_id: 2587354-4
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  • 13
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    Collective effects of common SNPs in foraging decisions in Caenorhabditis elegans and an integrative method of identification of candidate genes
    Springer Science and Business Media LLC ; 2015
    In:  Scientific Reports Vol. 5, No. 1 ( 2015-11-19)
    Details
    In: Scientific Reports, Springer Science and Business Media LLC, Vol. 5, No. 1 ( 2015-11-19)
    Abstract: Optimal foraging decision is a quantitative flexible behavior, which describes the time at which animals choose to abandon a depleting food supply. The total minor allele content (MAC) in an individual has been shown to correlate with quantitative variations in complex traits. We have studied the role of MAC in the decision to leave a food lawn in recombinant inbred advanced intercross lines (RIAILs) of Caenorhabditis elegans . We found a strong link between MAC and the food lawn leaving rates (Spearman r = 0.4, P  = 0.005). We identified 28 genes of unknown functions whose expression levels correlated with both MAC and leaving rates. When examined by RNAi experiments, 8 of 10 tested among the 28 affected leaving rates, whereas only 2 of 9 did among genes that were only associated with leaving rates but not MAC (8/10 vs 2/9, P   〈  0.05). The results establish a link between MAC and the foraging behavior and identify 8 genes that may play a role in linking MAC with the quantitative nature of the trait. The method of correlations with both MAC and traits may find broad applications in high efficiency identification of target genes for other complex traits in model organisms and humans.
    Type of Medium: Online Resource
    ISSN: 2045-2322
    URL: Article
    DOI: 10.1038/srep16904
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2015
    detail.hit.zdb_id: 2615211-3
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  • 14
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    Online Resource
    Compatibility between mitochondrial and nuclear genomes correlates with the quantitative trait of lifespan in Caenorhabditis elegans
    Springer Science and Business Media LLC ; 2015
    In:  Scientific Reports Vol. 5, No. 1 ( 2015-11-25)
    Details
    In: Scientific Reports, Springer Science and Business Media LLC, Vol. 5, No. 1 ( 2015-11-25)
    Abstract: Mutations in mitochondrial genome have epistatic effects on organisms depending on the nuclear background, but a role for the compatibility of mitochondrial-nuclear genomes (mit-n) in the quantitative nature of a complex trait remains unexplored. We studied a panel of recombinant inbred advanced intercrossed lines (RIAILs) of C. elegans that were established from a cross between the N2 and HW strains. We determined the HW nuclear genome content and the mitochondrial type (HW or N2) of each RIAIL strain. We found that the degree of mit-n compatibility was correlated with the lifespans but not the foraging behaviors of RIAILs. Several known aging-associated QTLs individually showed no relationship with mitotypes but collectively a weak trend consistent with a role in mit-n compatibility. By association mapping, we identified 293 SNPs that showed linkage with lifespan and a relationship with mitotypes consistent with a role in mit-n compatibility. We further found an association between mit-n compatibility and several functional characteristics of mitochondria as well as the expressions of genes involved in the respiratory oxidation pathway. The results provide the first evidence implicating mit-n compatibility in the quantitative nature of a complex trait and may be informative to certain evolutionary puzzles on hybrids.
    Type of Medium: Online Resource
    ISSN: 2045-2322
    URL: Article
    DOI: 10.1038/srep17303
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2015
    detail.hit.zdb_id: 2615211-3
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  • 15
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    Intestinal bacteria—a powerful weapon for fungal infections treatment
    Frontiers Media SA ; 2023
    In:  Frontiers in Cellular and Infection Microbiology Vol. 13 ( 2023-6-2)
    Details
    In: Frontiers in Cellular and Infection Microbiology, Frontiers Media SA, Vol. 13 ( 2023-6-2)
    Abstract: The morbidity and mortality of invasive fungal infections are rising gradually. In recent years, fungi have quietly evolved stronger defense capabilities and increased resistance to antibiotics, posing huge challenges to maintaining physical health. Therefore, developing new drugs and strategies to combat these invasive fungi is crucial. There are a large number of microorganisms in the intestinal tract of mammals, collectively referred to as intestinal microbiota. At the same time, these native microorganisms co-evolve with their hosts in symbiotic relationship. Recent researches have shown that some probiotics and intestinal symbiotic bacteria can inhibit the invasion and colonization of fungi. In this paper, we review the mechanism of some intestinal bacteria affecting the growth and invasion of fungi by targeting the virulence factors, quorum sensing system, secreting active metabolites or regulating the host anti-fungal immune response, so as to provide new strategies for resisting invasive fungal infection.
    Type of Medium: Online Resource
    ISSN: 2235-2988
    URL: Article
    DOI: 10.3389/fcimb.2023.1187831
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2023
    detail.hit.zdb_id: 2619676-1
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  • 16
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    A Non-enzymatic glucose sensor via uniform copper nanosphere fabricated by two-step method
    Elsevier BV ; 2021
    In:  Optics & Laser Technology Vol. 144 ( 2021-12), p. 107430-
    Details
    In: Optics & Laser Technology, Elsevier BV, Vol. 144 ( 2021-12), p. 107430-
    Type of Medium: Online Resource
    ISSN: 0030-3992
    URL: Article
    DOI: 10.1016/j.optlastec.2021.107430
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2021
    detail.hit.zdb_id: 2000654-8
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  • 17
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    Effect of trypsin concentration on living SMCC‐7721 cells studied by atomic force microscopy
    Wiley ; 2021
    In:  Journal of Microscopy Vol. 284, No. 3 ( 2021-12), p. 203-213
    Details
    In: Journal of Microscopy, Wiley, Vol. 284, No. 3 ( 2021-12), p. 203-213
    Abstract: Trypsin is playing an important role in the processes of cancer proliferation, invasion and metastasis which require the precise information of morphology and mechanical properties on the nano‐scale for the related research. In this work, living human hepatoma (SMCC‐7721) cells were treated with different concentrations of trypsin solution. The morphology and mechanical properties of the cells were measured via atomic force microscope (AFM). Statistical analyses of measurement data indicated that with the increase of trypsin concentration, the average cell height and the surface roughness were both increased, but the cell viability, the cell surface adhesion and the elasticity modulus were decreased significantly. The force required to puncture the cells was also gradually reduced. It indicates that trypsin not only hydrolyses the proteins between the cell and the substrate but also the membrane proteins. The results offer valuable clues for the cancerous process study, pathological analysis and trypsin inhibitor drug development. And this work provides an effective way for overcoming the cell membrane in drug injection for cell‐targeted therapy.
    Type of Medium: Online Resource
    ISSN: 0022-2720 , 1365-2818
    URL: Issue
    URL: Article
    DOI: 10.1111/jmi.v284.3
    DOI: 10.1111/jmi.13053
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 2007259-4
    SSG: 11
    SSG: 12
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  • 18
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    The effects of measurement parameters on the cancerous cell nucleus characterisation by atomic force microscopy in vitro
    Wiley ; 2022
    In:  Journal of Microscopy Vol. 287, No. 1 ( 2022-07), p. 3-18
    Details
    In: Journal of Microscopy, Wiley, Vol. 287, No. 1 ( 2022-07), p. 3-18
    Abstract: Cancer is now responsible for the majority leading cause of death worldwide and it is noteworthy that lung cancer has been recognised as the highest incidence (11.6%) and mortality (18.4%) for combined sexes among a variety of cancer diseases. Therefore, it is of great value to investigate the mechanical properties of lung cancerous cells for early diagnosis. This paper primarily investigated the morphological properties and the influence of measurement parameters on the measured local elastic moduli of single live A549 cell in vitro using the AFM‐based force spectroscopy mode. In practice, there are many factors for incorrect or inaccurate experimental results using AFM to measure the characteristics of live cells, such as non‐homogeneous nature of cells, probe geometry and size, mechanical analysis model, substrate stiffness and different measurement parameters. The various measurement parameters have become the huge impact factor to influence the measurement result. Hence, this research may have potential significance to provide reference for the standardised detection of a single cancerous cell in vitro using AFM methodologies.
    Type of Medium: Online Resource
    ISSN: 0022-2720 , 1365-2818
    URL: Issue
    URL: Article
    DOI: 10.1111/jmi.v287.1
    DOI: 10.1111/jmi.13104
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 2007259-4
    SSG: 11
    SSG: 12
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  • 19
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    Ion-modulated photoluminescence of MoS 2 nanoflakes for cell viability detection
    Royal Society of Chemistry (RSC) ; 2022
    In:  New Journal of Chemistry Vol. 46, No. 46 ( 2022), p. 22441-22450
    Details
    In: New Journal of Chemistry, Royal Society of Chemistry (RSC), Vol. 46, No. 46 ( 2022), p. 22441-22450
    Abstract: Cell viability detection plays a crucial role in apoptosis and anticancer drug research. Compared with conventional colorimetric assays and organic fluorescent probe-based imaging, it is greatly desired to develop a simple and facile fluorescent probe for in situ real-time detection of cell viability. Herein, MoS 2 nanoflakes are employed as fluorescent probes for detecting cancer cell viability by using their inherent tunable photoluminescence (PL) properties by ion intercalation. The appropriate amount of MoS 2 nanoflakes for A549 lung cancer cells was firstly determined by MTT assay. After MoS 2 nanoflakes successfully covered cells, the effects of the nanoflakes on cell morphology and mechanical properties were studied by atomic force microscopy (AFM). Then, the modulation of PL by potassium ions (K + ) from cells was investigated at different cell viabilities. The results showed that PL intensity increased as cell viability decreased. Finally, the nanoflakes were further used as fluorescent probes to assess the cell viability change induced by anticancer drugs. The results showed that with the increase of drug concentration, the fluorescence intensity increased, indicating a decrease in cell viability. The ion-modulated PL of MoS 2 nanoflakes makes MoS 2 a promising candidate for applications in anticancer drug screening.
    Type of Medium: Online Resource
    ISSN: 1144-0546 , 1369-9261
    URL: Article
    DOI: 10.1039/D2NJ04073E
    Language: English
    Publisher: Royal Society of Chemistry (RSC)
    Publication Date: 2022
    detail.hit.zdb_id: 1472933-7
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  • 20
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    AgPt/MoS 2 hybrid as electrochemical sensor for detecting H 2 O 2 release from living cells
    Royal Society of Chemistry (RSC) ; 2022
    In:  New Journal of Chemistry Vol. 46, No. 31 ( 2022), p. 15032-15041
    Details
    In: New Journal of Chemistry, Royal Society of Chemistry (RSC), Vol. 46, No. 31 ( 2022), p. 15032-15041
    Abstract: Hydrogen peroxide (H 2 O 2 ), as a biomarker in a series of physiological processes, plays a crucial role in investigating the pathogenesis of some diseases. The highly sensitive and selective detection of H 2 O 2 release from living cells has aroused tremendous research interests. Herein, a non-enzymatic electrochemical H 2 O 2 biosensor was constructed based on AgPt bimetallic nanoparticle-structured MoS 2 nanohybrid (AgPt/MoS 2 ) as an electrocatalyst. The AgPt/MoS 2 nanohybrid was successfully synthesized by a facile wet-chemical method. Compared with AgPt nanoparticles, the AgPt/MoS 2 nanohybrid exhibited significant enhancement in catalytic performance towards H 2 O 2 . The main factor is that MoS 2 nanosheets as a supporting material promote AgPt nanoparticles to expose more active sites. The electrochemical response of the AgPt/MoS 2 hybrid-based biosensor to H 2 O 2 was linear in the range of 20 μM–4 mM, and the detection limit was calculated as 1.0 μM. To prove practicality, the biosensor was further applied to detect H 2 O 2 release from living SMMC-7721 cells. These results indicate the potential applications of the developed biosensor in monitoring physiological processes.
    Type of Medium: Online Resource
    ISSN: 1144-0546 , 1369-9261
    URL: Article
    DOI: 10.1039/D2NJ02495K
    Language: English
    Publisher: Royal Society of Chemistry (RSC)
    Publication Date: 2022
    detail.hit.zdb_id: 1472933-7
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