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  • 11
    Online Resource
    Online Resource
    Heat stress and sexual reproduction in maize: unveiling the most pivotal factors and the biggest opportunities
    Oxford University Press (OUP) ; 2024
    In:  Journal of Experimental Botany ( 2024-01-06)
    Details
    In: Journal of Experimental Botany, Oxford University Press (OUP), ( 2024-01-06)
    Abstract: The escalation in the intensity, frequency, and duration of high temperature (HT) stress is currently unparalleled, which aggravates the challenges for crop production. Yet, the stage-dependent responses of sexual reproductive organs to HT stress at the morphological, physiological, and molecular levels, remain inadequately explored, particularly in pivotal staple crops. This review synthesized current knowledge regarding the mechanisms by which HT stress induces abnormalities and aberrations in reproductive growth and development, as well as alters the morphology and function of florets and their constituents, flowering patterns, and the processes of pollination and fertilization in maize (Zea mays L.). We identified the stage-specific sensitivities to HT stress through compiling and analyzing hundreds of lines of evidence, and accurately defined the sensitive period from days to hours timescale. The microspore tetrad phase of pollen development and anthesis (especially shortly after pollination) are most sensitive to HT stress, and even brief temperature spikes during these stages can lead to significant kernel loss. Unfortunately, these weak links are hidden and often neglected in practice. The impetuses behind the heat-induced impairments in seed set are closely related to carbon, reactive oxygen species, phytohormone signals, ion (e.g., Ca2+) homeostasis, plasma membrane structure and function, and others. At last, the recent advancements in understanding the genetic mechanisms underlying HT stress responses during maize sexual reproduction have been systematically summarized. This knowledge holds significant implications for the development of improved maize genotypes and effective crop management strategies to mitigate heat stress.
    Type of Medium: Online Resource
    ISSN: 0022-0957 , 1460-2431
    URL: Article
    DOI: 10.1093/jxb/erad506
    RVK:
    WA 15000
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2024
    detail.hit.zdb_id: 1466717-4
    SSG: 12
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  • 12
    Online Resource
    Online Resource
    B7-H4 Inhibits the Development of Primary Sjögren’s Syndrome by Regulating Treg Differentiation in NOD/Ltj Mice
    Hindawi Limited ; 2020
    In:  Journal of Immunology Research Vol. 2020 ( 2020-09-27), p. 1-13
    Details
    In: Journal of Immunology Research, Hindawi Limited, Vol. 2020 ( 2020-09-27), p. 1-13
    Abstract: Background . This study is aimed at exploring the role of B7-H4 in the pathogenesis of primary Sjögren’s syndrome (pSS) in NOD/Ltj mouse. Methods . B7-H4 expression in salivary glands was examined by IHC, and lymphocyte infiltration was showed by H & E. Next, anti-B7-H4 mAb or immunoglobulin isotype was injected into NOD/Ltj mice. Cytokine levels were measured by quantitative RT-PCR, and immunoglobulins were measured by ELISA. T cell subsets were analyzed by flow cytometry. Last, we treated NOD/Ltj mice with B7-H4Ig and control Ig. CD4+Foxp3+ T cells were assessed by immunohistochemistry. Two-tailed Student’s t -tests were used to detect the statistical difference in various measures between the two groups. Results . B7-H4 expression was remarkably reduced in salivary glands of NOD/Ltj mice at 15 weeks compared with the NOD/Ltj mice at 8 weeks. Anti-B7-H4 mAb treatment increased lymphocyte infiltration in salivary glands. Inflammatory cytokines including IL-12, IL-18, IL-1 α , TNF- α , IFN- α , and BAFF were upregulated markedly in anti-B7-H4 mAb-treated mice compared to IgG isotype-treated mice. Flow cytometry analysis showed that anti-B7-H4 mAb-treated mice had lower levels of CD4+Foxp3+/CD4+ T cells in spleen. Moreover, Foxp3 mRNA levels of salivary glands were diminished in anti-B7-H4 mAb-treated mice. Flow cytometry analysis showed that anti-B7-H4 mAb inhibited CD4+Foxp3+/CD4+ T cell production, while B7-H4Ig would promote naïve CD4+ T into Treg differentiation. Administration with B7-H4Ig displayed significantly decreased lymphocyte infiltration in salivary glands and low levels of total IgM and IgG in serum. Analysis of inflammatory cytokines in salivary glands after B7-H4Ig treatment revealed that the mRNA levels of IL-12, IL-6, IL-18, IL-1 α , TNF- α , and IFN- α were significantly downregulated in B7-H4Ig-treated mice compared to control Ig treatment. B7-H4Ig-treated mice had significantly higher levels of CD4+Foxp3+/CD4+ T cells in spleen. IHC in salivary gland revealed that CD4+Foxp3+ T cells of B7-H4Ig treatment mouse were more than control Ig treatment. Conclusions . Our findings implicate that B7-H4 has a protective role for salivary gland epithelial cells (SGECs) and therapeutic potential in the treatment of pSS.
    Type of Medium: Online Resource
    ISSN: 2314-8861 , 2314-7156
    URL: Article
    DOI: 10.1155/2020/4896727
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2020
    detail.hit.zdb_id: 2817541-4
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  • 13
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    Effects of chronic sleep restriction on the neuro‐phenotypes of Ctnnd2 knockout mice
    Wiley ; 2023
    In:  Brain and Behavior Vol. 13, No. 7 ( 2023-07)
    Details
    In: Brain and Behavior, Wiley, Vol. 13, No. 7 ( 2023-07)
    Abstract: Sleep abnormalities are highly correlated with neurodevelopmental disorders, such as intellectual disability, attention deficit hyperactivity disorder, and autism spectrum disorders (ASD). The severity of behavioral abnormalities is correlated with the presence of sleep abnormalities. Based on previous research, we investigated that Ctnnd2 gene deletion in mice lead to ASD‐like behaviors and cognitive defects. Given the importance of sleep in individuals with ASD, this study aimed to determine the effects of chronic sleep restriction (SR) on wild‐type (WT) mice and on Ctnnd2 deletion‐induced, neurologically related phenotypes in mice. Method WT and Ctnnd2 knockout (KO) mice were both subjected to manual SR (5 h per day) for 21 consecutively days separately, then we compared neurologically related phenotypes of WT mice, WT mice subjected to SR, KO mice, and KO mice subjected to SR using a three‐chamber assay, direct social interaction test, open‐field test, Morris water maze, Golgi staining, and Western blotting. Results The effects of SR on WT and KO mice were different. After SR, social ability and cognition were impaired in both WT and KO mice. Repetitive behaviors were increased, and exploration abilities were decreased in KO mice but not in WT mice. Moreover, SR reduced the density and area of mushroom‐type dendritic spines in WT rather than KO mice. Finally, the PI3K/Akt‐mTOR pathway was found to be involved in the effects induced by SR‐impaired phenotypes in WT and KO mice. Conclusion Overall, results of the present study may have implications for the role of disrupted sleep in patients with CTNND2 gene‐related autism and the evolution of neurodevelopmental disorders.
    Type of Medium: Online Resource
    ISSN: 2162-3279 , 2162-3279
    URL: Issue
    URL: Article
    DOI: 10.1002/brb3.v13.7
    DOI: 10.1002/brb3.3075
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2623587-0
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  • 14
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    E3 ubiquitin ligase gene CMPG 1–V from Haynaldia villosa L. contributes to powdery mildew resistance in common wheat ( Triticum aestivum L.)
    Wiley ; 2015
    In:  The Plant Journal Vol. 84, No. 1 ( 2015-10), p. 154-168
    Details
    In: The Plant Journal, Wiley, Vol. 84, No. 1 ( 2015-10), p. 154-168
    Abstract: Powdery mildew causes significant yield decreases in wheat and the need for additional sources of resistance is ongoing. Here we show that a gene from a diploid wheat relative improved broad spectrum resistance to powdery mildew in cultivated wheat and thus is a resource for wheat breeding.
    Type of Medium: Online Resource
    ISSN: 0960-7412 , 1365-313X
    URL: Issue
    URL: Article
    DOI: 10.1111/tpj.2015.84.issue-1
    DOI: 10.1111/tpj.12966
    Language: English
    Publisher: Wiley
    Publication Date: 2015
    detail.hit.zdb_id: 2020961-7
    SSG: 12
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  • 15
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    Anaerobic fermentation of organic solid waste: Recent updates in substrates, products, and the process with multiple products co-production
    Elsevier BV ; 2023
    In:  Environmental Research Vol. 233 ( 2023-09), p. 116444-
    Details
    In: Environmental Research, Elsevier BV, Vol. 233 ( 2023-09), p. 116444-
    Type of Medium: Online Resource
    ISSN: 0013-9351
    URL: Article
    DOI: 10.1016/j.envres.2023.116444
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2023
    detail.hit.zdb_id: 205699-9
    detail.hit.zdb_id: 1467489-0
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  • 16
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    Bilayer Zwitterionic Metal‐Organic Framework for Selective All‐Solid‐State Superionic Conduction in Lithium Metal Batteries
    Wiley ; 2023
    In:  Advanced Materials Vol. 35, No. 39 ( 2023-09)
    Details
    In: Advanced Materials, Wiley, Vol. 35, No. 39 ( 2023-09)
    Abstract: Solid‐state batteries (SSBs) hold immense potential for improved energy density and safety compared to traditional batteries. However, existing solid‐state electrolytes (SSEs) face challenges in meeting the complex operational requirements of SSBs. This study introduces a novel approach to address this issue by developing a metal‐organic framework (MOF) with customized bilayer zwitterionic nanochannels (MOF‐BZN) as high‐performance SSEs. The BZN consist of a rigid anionic MOF channel with chemically grafted soft multicationic oligomers (MCOs) on the pore wall. This design enables selective superionic conduction, with MCOs restricting the movement of anions while coulombic interaction between MCOs and anionic framework promoting the dissociation of Li + . MOF‐BZN exhibits remarkable Li + conductivity (8.76 × 10 −4 S cm −1 ), high Li + transference number (0.75), and a wide electrochemical window of up to 4.9 V at 30 °C. Ultimately, the SSB utilizing flame retarded MOF‐BZN achieves an impressive specific energy of 419.6 Wh kg anode+cathode+electrolyte −1 under constrained conditions of high cathode loading (20.1 mg cm −2 ) and limited lithium metal source. The constructed bilayer zwitterionic MOFs present a pioneering strategy for developing advanced SSEs for highly efficient SSBs.
    Type of Medium: Online Resource
    ISSN: 0935-9648 , 1521-4095
    URL: Issue
    URL: Article
    DOI: 10.1002/adma.v35.39
    DOI: 10.1002/adma.202304685
    RVK:
    UA 1538
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 1474949-X
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  • 17
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    Global N6-Methyladenosine Profiling Revealed the Tissue-Specific Epitranscriptomic Regulation of Rice Responses to Salt Stress
    MDPI AG ; 2022
    In:  International Journal of Molecular Sciences Vol. 23, No. 4 ( 2022-02-14), p. 2091-
    Details
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 23, No. 4 ( 2022-02-14), p. 2091-
    Abstract: N6-methyladenosine (m6A) methylation represents a new layer of the epitranscriptomic regulation of plant development and growth. However, the effects of m6A on rice responses to environmental stimuli remain unclear. In this study, we performed a methylated-RNA immunoprecipitation sequencing analysis and compared the changes in m6A methylation and gene expression in rice under salt stress conditions. Salt stress significantly increased the m6A methylation in the shoots (p value 〈 0.05). Additionally, 2537 and 2304 differential m6A sites within 2134 and 1997 genes were identified in the shoots and roots, respectively, under salt stress and control conditions. These differential m6A sites were largely regulated in a tissue-specific manner. A unique set of genes encoding transcription factors, antioxidants, and auxin-responsive proteins had increased or decreased m6A methylation levels only in the shoots or roots under salt stress, implying m6A may mediate salt tolerance by regulating transcription, ROS homeostasis, and auxin signaling in a tissue-specific manner. Integrating analyses of m6A modifications and gene expression changes revealed that m6A changes regulate the expression of genes controlling plant growth, stress responses, and ion transport under saline conditions. These findings may help clarify the regulatory effects of m6A modifications on rice salt tolerance.
    Type of Medium: Online Resource
    ISSN: 1422-0067
    URL: Article
    DOI: 10.3390/ijms23042091
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2019364-6
    SSG: 12
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  • 18
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    OPTIMUM DESIGN FOR BREAKING DEVICE WITH DOUBLE ROLLER BASED EDEM
    INMA Bucharest-Romania ; 2023
    In:  INMATEH Agricultural Engineering
    Details
    In: INMATEH Agricultural Engineering, INMA Bucharest-Romania
    Abstract: Straw found in the field can be conveniently stored and transported through mechanical packaging, and it undergoes crushing during its utilization. The effectiveness of the crushing process directly impacts the efficiency of material utilization. However, current crushing devices available in the market lack specialized mechanisms suitable for the unique characteristics of round corn stalks. To address this issue, the necessary simulation parameters were determined, and an experiment was conducted using the ternary quadratic combination test method. The test factors considered were the center height difference of the knife roller, lower roller speed, and cutter thickness. The response value of the experiment was the material crushing rate. By analyzing the results, the optimal structural parameters were identified. These included a center height difference of 390 mm for the knife roller, a lower roller speed of 14 rpm, and a cutter thickness of 110 mm. With these parameters, the crushing rate of the round straw crushing device reached 93.77%, and the particle passing ratio was 98.52%. To validate the research findings, a test device was constructed and used to confirm the reliability of the obtained results.
    Type of Medium: Online Resource
    ISSN: 2068-2239 , 2068-4215
    Uniform Title: 基于 EDEM 的双滚轮破碎装置优化设计
    URL: Journal
    URL: Article
    DOI: 10.35633/INMATEH
    DOI: 10.35633/inmateh-70-55
    Language: English
    Publisher: INMA Bucharest-Romania
    Publication Date: 2023
    detail.hit.zdb_id: 2780406-9
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  • 19
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    Integrative analysis of pooled CRISPR genetic screens using MAGeCKFlute
    Springer Science and Business Media LLC ; 2019
    In:  Nature Protocols Vol. 14, No. 3 ( 2019-3), p. 756-780
    Details
    In: Nature Protocols, Springer Science and Business Media LLC, Vol. 14, No. 3 ( 2019-3), p. 756-780
    Type of Medium: Online Resource
    ISSN: 1754-2189 , 1750-2799
    URL: Article
    DOI: 10.1038/s41596-018-0113-7
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2019
    detail.hit.zdb_id: 2244966-8
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  • 20
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    Elevated atmospheric CO 2 concentration triggers redistribution of nitrogen to promote tillering in rice
    Wiley ; 2021
    In:  Plant-Environment Interactions Vol. 2, No. 3 ( 2021-06), p. 125-136
    Details
    In: Plant-Environment Interactions, Wiley, Vol. 2, No. 3 ( 2021-06), p. 125-136
    Abstract: Elevated atmospheric CO 2 concentration (eCO 2 ) often reduces nitrogen (N) content in rice plants and stimulates tillering. However, there is a general consensus that reduced N would constrain rice tillering. To resolve this contradiction, we investigated N distribution and transcriptomic changes in different rice plant organs after subjecting them to eCO 2 and different N application rates. Our results showed that eCO 2 significantly promoted rice tillers (by 0.6, 1.1, 1.7, and 2.1 tillers/plant at 0, 75, 150, and 225 kg N ha −1  N application rates, respectively) and more tillers were produced under higher N application rates, confirming that N availability constrained tillering in the early stages of growth. Although N content declined in the leaves (−11.0 to −20.7 mg g −1 ) and sheaths (−9.8 to −28.8 mg g −1 ) of rice plants exposed to eCO 2 , the N content of newly emerged tillers on plants exposed to eCO 2 equaled or exceeded the N content of tillers produced under ambient CO 2 conditions. Apparently, the redistribution of N within the plant per se was a critical adaptation strategy to the eCO 2 condition. Transcriptomic analysis revealed that eCO 2 induced less extensive alteration of gene expression than did N application. Most importantly, the expression levels of multiple N‐related transporters and receptors such as nitrate transporter NRT2.3a/b and NRT1.1a/b were differentially regulated in leaf and shoot apical meristem, suggesting that multiple genes were involved in sensing the N signal and transporting N metabolites to adapt to eCO 2 . The redistribution of N in different organs could be a universal adaptation strategy of terrestrial plants to eCO 2 .
    Type of Medium: Online Resource
    ISSN: 2575-6265 , 2575-6265
    URL: Issue
    URL: Article
    DOI: 10.1002/pei3.v2.3
    DOI: 10.1002/pei3.10046
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 3029697-3
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