In:
Virology Journal, Springer Science and Business Media LLC, Vol. 6, No. 1 ( 2009-12)
Abstract:
We conducted molecular analyses to confirm four clustering HIV-1 infections (Patient A, B, C & D) in Guangzhou, China. These cases were identified by epidemiological investigation and suspected to acquire the infection through a common heterosexual transmission chain. Methods Env C2V3V4 region, gag p17/p24 junction and partial pol gene of HIV-1 genome from serum specimens of these infected cases were amplified by reverse transcription polymerase chain reaction (RT-PCR) and nucleotide sequenced. Results Phylogenetic analyses indicated that their viral nucleotide sequences were significantly clustered together (bootstrap value is 99%, 98% and 100% in env , gag and pol tree respectively). Evolutionary distance analysis indicated that their genetic diversities of env , gag and pol genes were significantly lower than non-clustered controls, as measured by unpaired t -test ( env gene comparison: p 〈 0.005; gag gene comparison: p 〈 0.005; pol gene comparison: p 〈 0.005). Conclusion Epidemiological results and molecular analyses consistently illustrated these four cases represented a transmission chain which dispersed in the locality through heterosexual contact involving commercial sex worker.
Type of Medium:
Online Resource
ISSN:
1743-422X
DOI:
10.1186/1743-422X-6-148
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2009
detail.hit.zdb_id:
2160640-7
Permalink