In:
PLOS ONE, Public Library of Science (PLoS), Vol. 18, No. 6 ( 2023-6-27), p. e0287534-
Abstract:
Currently, comorbidities of obesity are becoming increasingly frequent. For example, obese women are more susceptible to reproductive diseases; however, the underlying mechanism remains poorly understood. The present study aimed to explore the effect of obesity on female reproduction and discuss changes of the lipid profile in ovarian granulosa cells. Fifty female mice were randomly divided into two groups, one group was fed high-fat diet, the other group was fed standard control diet, food and water freely. After 12 weeks of feeding, the average body weight of the high-fat diet mice (19.027g) was significantly higher than that of the standard control diet mice (36.877g) (P 〈 0.05). The tissue sections were stained with oil red O, and the online software mage Pro plus 6 . 0 analyzed the staining results, the lipids in the ovaries and endometria were found to be different between the two groups. Liquid chromatography-electrospray ionization with tandem mass spectrometry (LC-ESI-MS/MS) analysis of ovarian granulosa cells (GCs) was performed, with a total of 228 different lipids being identified, the abundant of 147 were increased and 81 were decreased in the high-fat diet group. Among them, PI (18:1/20:1) was the most different lipid, and high-fat feeding was 85 times higher than standard control group. Among these different lipids, 44% in phospholipid metabolism, 30% in glycerolipid metabolism, and 30% in fat digestion and absorption. The results of this study laid a theoretical foundation of the effects of diet-induced obesity on female reproduction.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0287534
DOI:
10.1371/journal.pone.0287534.g001
DOI:
10.1371/journal.pone.0287534.g002
DOI:
10.1371/journal.pone.0287534.g003
DOI:
10.1371/journal.pone.0287534.g004
DOI:
10.1371/journal.pone.0287534.g005
DOI:
10.1371/journal.pone.0287534.g006
DOI:
10.1371/journal.pone.0287534.t001
DOI:
10.1371/journal.pone.0287534.s001
DOI:
10.1371/journal.pone.0287534.s002
DOI:
10.1371/journal.pone.0287534.s003
DOI:
10.1371/journal.pone.0287534.s004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2267670-3
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