In:
PLOS Genetics, Public Library of Science (PLoS), Vol. 18, No. 11 ( 2022-11-21), p. e1010289-
Abstract:
The Serotonin Transporter (SERT) regulates extracellular serotonin levels and is the target of most current drugs used to treat depression. The mechanisms by which inhibition of SERT activity influences behavior are poorly understood. To address this question in the model organism Drosophila melanogaster , we developed new loss of function mutations in Drosophila SERT ( dSERT) . Previous studies in both flies and mammals have implicated serotonin as an important neuromodulator of sleep, and our newly generated dSERT mutants show an increase in total sleep and altered sleep architecture that is mimicked by feeding the SSRI citalopram. Differences in daytime versus nighttime sleep architecture as well as genetic rescue experiments unexpectedly suggest that distinct serotonergic circuits may modulate daytime versus nighttime sleep. dSERT mutants also show defects in copulation and food intake, akin to the clinical side effects of SSRIs and consistent with the pleomorphic influence of serotonin on the behavior of D . melanogaster . Starvation did not overcome the sleep drive in the mutants and in male dSERT mutants, the drive to mate also failed to overcome sleep drive. dSERT may be used to further explore the mechanisms by which serotonin regulates sleep and its interplay with other complex behaviors.
Type of Medium:
Online Resource
ISSN:
1553-7404
DOI:
10.1371/journal.pgen.1010289
DOI:
10.1371/journal.pgen.1010289.g001
DOI:
10.1371/journal.pgen.1010289.g002
DOI:
10.1371/journal.pgen.1010289.g003
DOI:
10.1371/journal.pgen.1010289.g004
DOI:
10.1371/journal.pgen.1010289.g005
DOI:
10.1371/journal.pgen.1010289.g006
DOI:
10.1371/journal.pgen.1010289.g007
DOI:
10.1371/journal.pgen.1010289.s001
DOI:
10.1371/journal.pgen.1010289.s002
DOI:
10.1371/journal.pgen.1010289.s003
DOI:
10.1371/journal.pgen.1010289.s004
DOI:
10.1371/journal.pgen.1010289.s005
DOI:
10.1371/journal.pgen.1010289.s006
DOI:
10.1371/journal.pgen.1010289.s007
DOI:
10.1371/journal.pgen.1010289.r001
DOI:
10.1371/journal.pgen.1010289.r002
DOI:
10.1371/journal.pgen.1010289.r003
DOI:
10.1371/journal.pgen.1010289.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2186725-2
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