In:
The Plant Pathology Journal, Korean Society of Plant Pathology, Vol. 38, No. 5 ( 2022-10-01), p. 522-532
Abstract:
Gummy stem blight (GSB), a common and serious disease in cucurbits worldwide, is caused by three genetically distinct species: 〈 i 〉 Stagonosporopsis cucurbitacearum 〈 /i 〉 (syn. 〈 i 〉 Didymella bryoniae 〈 /i 〉 ), 〈 i 〉 S. citrulli 〈 /i 〉 , and 〈 i 〉 S. caricae 〈 /i 〉 . In Korea, however, the three species of 〈 i 〉 Stagonosporopsis 〈 /i 〉 have been barely characterized. In this study, 21 〈 i 〉 Stagonosporopsis 〈 /i 〉 isolates were recovered from watermelon ( 〈 i 〉 Citrullus lanatus 〈 /i 〉 ) and muskmelon ( 〈 i 〉 Cucumis melo 〈 /i 〉 ) leaves and stem showing blight symptoms collected from 43 fields in Korea. Sequence analysis performed with an internal transcribed spacer region was not competent to differentiate the 〈 i 〉 Stagonosporopsis 〈 /i 〉 isolates. On the contrary, analysis of β-tubulin ( 〈 i 〉 TUB 〈 /i 〉 ) genes and three microsatellite markers, Db01, Db05, and Db06, successfully differentiated 〈 i 〉 Stagonosporopsis 〈 /i 〉 isolates. Further sequence analysis identified two 〈 i 〉 Stagonosporopsis 〈 /i 〉 species, 〈 i 〉 S. citrulli 〈 /i 〉 and 〈 i 〉 S. caricae 〈 /i 〉 , and one previously unknown species of 〈 i 〉 Stagonosporopsis 〈 /i 〉 . Representative isolates from three species caused dark water-soaked lesions on the detached watermelon and muskmelon leaves with no significant differences in the aggressiveness. Our results indicate that the 〈 i 〉 S. citrulli 〈 /i 〉 , 〈 i 〉 S. caricae 〈 /i 〉 , and unknown 〈 i 〉 Stagonosporopsis 〈 /i 〉 sp. are all causal agents of GSB for both watermelon and muskmelon. This is the first report of a new species and the population structure of 〈 i 〉 Stagonosporopsis 〈 /i 〉 species causing GSB in Korea.
Type of Medium:
Online Resource
ISSN:
1598-2254
,
2093-9280
DOI:
10.5423/PPJ.OA.07.2022.0102
Language:
English
Publisher:
Korean Society of Plant Pathology
Publication Date:
2022
detail.hit.zdb_id:
2529195-6
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