In:
APMIS, Wiley, Vol. 124, No. 3 ( 2016-03), p. 175-180
Kurzfassung:
Anal intraepithelial neoplasia ( AIN ) is a precursor to invasive anal squamous cell carcinoma. Histologic evaluation is hampered by intra‐ and interobserver variability. Various biomarkers have been investigated to improve the accuracy and reproducibility of diagnosis and grading, but interpretation can be challenging. ProEx ™ C is an antibody cocktail for proteins upregulated in cervical intraepithelial neoplasia. This study investigated ProEx ™ C's role alone and with p16 and Ki‐67 in the diagnosis and grading of AIN . Sixty‐seven anal tissue samples (22 AIN I, 25 AIN II / III , and 20 non‐dysplastic) were stained for ProEx ™ C, Ki‐67, and p16. Staining patterns were recorded and correlated with morphologic diagnoses. Considering AIN II / III vs I, full‐thickness ProEx ™ C staining was more frequent in AIN II / III (p = 0.0373), and showed the highest sensitivity of the biomarkers. In combination with Ki‐67, sensitivity was lower, but specificity for AIN II / III rose to 83%. For differentiating non‐dysplasia from AIN I, negative ProEx ™ C staining correlated with non‐dysplasia (p 〈 0.0001) and had the highest sensitivity (90%). In combination with Ki‐67, sensitivity dropped to 80%, but specificity was high (96%). ProEx ™ C is useful for diagnosing and grading AIN , performing as well or better than other markers at identifying AIN II / III and non‐dysplastic epithelium.
Materialart:
Online-Ressource
ISSN:
0903-4641
,
1600-0463
DOI:
10.1111/apm.2016.124.issue-3
Sprache:
Englisch
Verlag:
Wiley
Publikationsdatum:
2016
ZDB Id:
2098213-6
SSG:
12
Permalink