In:
Acta Parasitologica, Springer Science and Business Media LLC, Vol. 63, No. 2 ( 2018-06-26), p. 397-411
Abstract:
Toxoplasma gondii is an obligate intracellular parasite with worldwide distribution. Virulence of T . gondii is a multigenic trait. Genetic and virulence data for T . gondii isolates from humans and animals in China have been reported. However, almost all biological materials used for genotyping of T . gondii from humans and pigs were DNA samples prepared from tissues, and T. gondii strains used for virulence analysis were isolated mainly from cats. In this study, one isolate from a dead human fetus was identified as type I (ToxoDB #10) while the two isolates from dead pigs were type Chinese I (ToxoDB #9) with PCR-restriction fragment length polymorphism using 10 markers ( SAG1 , SAG2 , SAG3 , BTUB , GRA6 , c22 – 8 , c29 – 2 , L358 , PK1 and Apico). Three isolates were comfirmed as virulent strains in mice. By cloning and sequences analysis, all isolates contained a Pvu II restriction site (572–577 bp) in the KHB fragment and five tandem repeats in the 5′ UTR region of SAG1 , which were associated with T . gondii virulence. The type Chinese I isolates contained two deletions of 15 and 3 bp at positions 635 to 649 and 658 to 660 in the GRA6 , which were correlated with genotype, but not with virulence. To our knowledge, this is the first report on the systematic analysis of murine virulence of type Chinese I strain from pigs, and the associations of sequences of the KHB fragment and SAG1 with virulence of type Chinese I strain. The Chinese I genotype was more closely related to type II strains.
Type of Medium:
Online Resource
ISSN:
1896-1851
,
1230-2821
DOI:
10.1515/ap-2018-0046
Language:
Unknown
Publisher:
Springer Science and Business Media LLC
Publication Date:
2018
detail.hit.zdb_id:
2235366-5
SSG:
12
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