In:
FEBS Letters, Wiley, Vol. 508, No. 2 ( 2001-11-16), p. 191-195
Abstract:
Soybean cell suspension cultures have been used to investigate the role of the elevation of the cytosolic Ca 2+ concentration in β‐glucan elicitors‐induced defence responses, such as H 2 O 2 and phytoalexin production. The intracellular Ca 2+ concentration was monitored in transgenic cells expressing the Ca 2+ ‐sensing aequorin. Two lines of evidence showed that a transient increase of the cytosolic Ca 2+ concentration is not necessarily involved in the induction of H 2 O 2 generation: (i) a Bradyrhizobium japonicum cyclic β‐glucan induced the H 2 O 2 burst without increasing the cytosolic Ca 2+ concentration; (ii) two ion channel blockers (anthracene‐9‐carboxylate, A9C; 5‐nitro‐2‐(3‐phenylpropylamino)‐benzoate, NPPB) could not prevent a Phytophthora soja β‐glucan elicitor‐induced H 2 O 2 synthesis but did prevent a cytosolic Ca 2+ concentration increase. Moreover, A9C and NPPB inhibited P. sojae β‐glucan‐elicited defence‐related gene inductions as well as the inducible accumulation of phytoalexins, suggesting that the P. sojae β‐glucan‐induced transient cytosolic Ca 2+ increase is not necessary for the elicitation of H 2 O 2 production but is very likely required for phytoalexin synthesis
Type of Medium:
Online Resource
ISSN:
0014-5793
,
1873-3468
DOI:
10.1016/S0014-5793(01)03054-X
Language:
English
Publisher:
Wiley
Publication Date:
2001
detail.hit.zdb_id:
1460391-3
SSG:
12
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