In:
Journal of the Chinese Chemical Society, Wiley, Vol. 53, No. 6 ( 2006-12), p. 1303-1308
Abstract:
A strong fluorescence probe has been designed to investigate cytochrome c folding kinetics. The cysteine specific modifier, 2‐[(‐Iodo‐acetyl)‐methyl‐amino]‐6‐methylaminobenzoic acidmethyl ester ( 1 ), has been prepared, and the reaction with cysteine can be performed in aqueous solution to ensure its application to protein modification. The model compound, 2‐{[2‐(2‐Acetylamino‐2‐methyoxycarbonyl‐ethylsulfanyl)‐acetyl]‐methyl‐amino}‐6‐methyl amino‐benzoic acid methyl ester ( 2 ), resulting from the reaction of 1 with N‐acetyl cysteine has emission quantum yields of 0.627 and 0.185 in nonaqueous and aqueous buffer solutions, respectively. The lifetime of 4.6 ns for compound 2 manifests a singlet excited‐state. The Förster energy transfer distance of compound 2 and cytochrome c is calculated to be 45 Å.
Type of Medium:
Online Resource
ISSN:
0009-4536
,
2192-6549
DOI:
10.1002/jccs.200600174
Language:
English
Publisher:
Wiley
Publication Date:
2006
detail.hit.zdb_id:
2029538-8
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