In:
Scientific Reports, Springer Science and Business Media LLC, Vol. 7, No. 1 ( 2017-06-28)
Abstract:
Asymmetric cell divisions are required for cellular diversity and defects can lead to altered daughter cell fates and numbers. In a genetic screen for C . elegans mutants with defects in dopaminergic head neuron specification or differentiation, we isolated a new allele of the transcription factor HAM-1 [HSN (Hermaphrodite-Specific Neurons) Abnormal Migration]. Loss of both HAM-1 and its target, the kinase PIG-1 [PAR-1(I)-like Gene] , leads to abnormal dopaminergic head neuron numbers. We identified discrete genetic relationships between ham - 1 , pig - 1 and apoptosis pathway genes in dopaminergic head neurons. We used an unbiased, quantitative mass spectrometry-based proteomics approach to characterise direct and indirect protein targets and pathways that mediate the effects of PIG-1 kinase loss in C . elegans embryos. Proteins showing changes in either abundance, or phosphorylation levels, between wild-type and pig - 1 mutant embryos are predominantly connected with processes including cell cycle, asymmetric cell division, apoptosis and actomyosin-regulation. Several of these proteins play important roles in C . elegans development. Our data provide an in-depth characterisation of the C . elegans wild-type embryo proteome and phosphoproteome and can be explored via the Encyclopedia of Proteome Dynamics (EPD) – an open access, searchable online database.
Type of Medium:
Online Resource
ISSN:
2045-2322
DOI:
10.1038/s41598-017-04375-4
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2017
detail.hit.zdb_id:
2615211-3
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