In:
PLOS Biology, Public Library of Science (PLoS), Vol. 19, No. 12 ( 2021-12-20), p. e3001426-
Abstract:
This work addresses the need for new chemical matter in product development for control of pest insects and vector-borne diseases. We present a barcoding strategy that enables phenotypic screens of blood-feeding insects against small molecules in microtiter plate-based arrays and apply this to discovery of novel systemic insecticides and compounds that block malaria parasite development in the mosquito vector. Encoding of the blood meals was achieved through recombinant DNA-tagged Asaia bacteria that successfully colonised Aedes and Anopheles mosquitoes. An arrayed screen of a collection of pesticides showed that chemical classes of avermectins, phenylpyrazoles, and neonicotinoids were enriched for compounds with systemic adulticide activity against Anopheles . Using a luminescent Plasmodium falciparum reporter strain, barcoded screens identified 48 drug-like transmission-blocking compounds from a 400-compound antimicrobial library. The approach significantly increases the throughput in phenotypic screening campaigns using adult insects and identifies novel candidate small molecules for disease control.
Type of Medium:
Online Resource
ISSN:
1545-7885
DOI:
10.1371/journal.pbio.3001426
DOI:
10.1371/journal.pbio.3001426.g001
DOI:
10.1371/journal.pbio.3001426.g002
DOI:
10.1371/journal.pbio.3001426.g003
DOI:
10.1371/journal.pbio.3001426.g004
DOI:
10.1371/journal.pbio.3001426.s001
DOI:
10.1371/journal.pbio.3001426.s002
DOI:
10.1371/journal.pbio.3001426.s003
DOI:
10.1371/journal.pbio.3001426.s004
DOI:
10.1371/journal.pbio.3001426.s005
DOI:
10.1371/journal.pbio.3001426.s006
DOI:
10.1371/journal.pbio.3001426.s007
DOI:
10.1371/journal.pbio.3001426.s008
DOI:
10.1371/journal.pbio.3001426.s009
DOI:
10.1371/journal.pbio.3001426.s010
DOI:
10.1371/journal.pbio.3001426.s011
DOI:
10.1371/journal.pbio.3001426.s012
DOI:
10.1371/journal.pbio.3001426.s013
DOI:
10.1371/journal.pbio.3001426.s014
DOI:
10.1371/journal.pbio.3001426.s015
DOI:
10.1371/journal.pbio.3001426.r001
DOI:
10.1371/journal.pbio.3001426.r002
DOI:
10.1371/journal.pbio.3001426.r003
DOI:
10.1371/journal.pbio.3001426.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2126773-X
Permalink