In:
American Journal of Veterinary Research, American Veterinary Medical Association (AVMA), Vol. 64, No. 10 ( 2003-10-01), p. 1213-1220
Abstract:
Objective —To determine the anti-inflammatory
effects of glycyrrhizin (GL) in lactating cows with mastitis attributable to naturally occurring infection with
coagulase-negative staphylococci (CNS). Animals —12 lactating Holstein cows with mastitis
attributable to infection with CNS and 2 healthy cows without mastitis. Procedure —Clinical signs, number of bacteria in milk,
somatic cell count (SCC) in milk, concentrations of α-lactalbumin and lactoferrin in milk, and concentration
of histamine in milk were investigated before and after intramammary infusion of GL (6 cows) or antimicrobials
(6 cows). Glands of 2 healthy cows were infused with staphylococcal enterotoxin; milk leukocytes
were then harvested and incubated with various doses of GL. Results —In cows infected with CNS that had a low
bacterial concentration in milk, infusion of GL alone resulted in significant improvements in swelling, firmness
of glands, and number of clots in milk, and it decreased the SCC, but not significantly. Percentage
of neutrophils decreased significantly (to 〈 30%) by 2
days after infusion. Use of lactoferrin as a marker of inflammation in mammary glands revealed a
decrease in concentrations, whereas use of α-lactalbumin as a marker of recovery for mammary glands
revealed significant increases in concentrations in the GL-infused group. Accompanying these anti-inflammatory
effects, a decrease in the concentration of histamine in milk was observed in the GL-infused group.
Glycyrrhizin decreased histamine production by milk leukocytes in a concentration-dependent manner. Conclusions and Clinical Relevance —Infusion of
GL may regulate intramammary inflammation through modulation of inflammatory mediators such as histamine.
( Am J Vet Res 2003;64:1213–1220)
Type of Medium:
Online Resource
ISSN:
0002-9645
DOI:
10.2460/ajvr.2003.64.1213
Language:
Unknown
Publisher:
American Veterinary Medical Association (AVMA)
Publication Date:
2003
detail.hit.zdb_id:
2056942-7
SSG:
22
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