In:
Evidence-Based Complementary and Alternative Medicine, Hindawi Limited, Vol. 2019 ( 2019-08-25), p. 1-9
Abstract:
Object . To investigate the effect of Kuijieling (KJL) on the balance between T helper 17 (Th17) and regulatory T (Treg) cells in peripheral blood mononuclear cells (PBMC) in vitro and explore the underlying mechanism. Materials and Methods . PBMCs isolated from rats were stimulated with transforming growth factor- β , interleukin (IL)-6, and IL-23 to induce the imbalance of Th17 and Treg cells and were treated with 10, 5, or 2.5% KJL-containing serum. The proportion of Th17 or Treg cells in CD4 + T cells was analyzed by flow cytometry, the concentrations of IL-17, IL-21, and IL-10 were assayed by ELISA, mRNA expressions of retinoic acid-related orphan receptor γ t (ROR γ t), forkhead box protein 3 (Foxp3), and signal transducer and activator of transcription 3 (STAT3) were quantified by PCR, and phosphorylated STAT3 (p-STAT3) was analyzed by flow cytometry. Results . KJL-containing serum decreased the proportion of Th17 cells and increased the proportion of Treg cells in CD4 + T cells, decreased the concentration of IL-17 and IL-21, enhanced the level of IL-10 in the cell culture supernatant, promoted the expression of Foxp3, and inhibited the levels of ROR γ t, STAT3, and p-STAT3. Conclusion . KJL suppresses the STAT3 pathway to remedy the imbalance between Th17 and Treg cells.
Type of Medium:
Online Resource
ISSN:
1741-427X
,
1741-4288
DOI:
10.1155/2019/7837989
Language:
English
Publisher:
Hindawi Limited
Publication Date:
2019
detail.hit.zdb_id:
2148302-4
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