In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 63, No. Pt_11 ( 2013-11-01), p. 4259-4265
Abstract:
Gram-negative, rod-shaped, oxidase-negative, facultatively anaerobic, yellow–orange-pigmented and motile bacterial strains, designated 8N4 T , 9N2 and 10N3, were isolated from flower nectar of Amygdalus communis (almond) and Citrus paradisi (grapefruit). The 16S rRNA gene sequences of the strains shared highest sequence similarity of 97.0 % with that of Phaseolibacter flectens ATCC 12775 T and lower similarity with sequences from other type strains of genera of the Enterobacteriaceae . A polyphasic approach that included determination of phenotypic properties and phylogenetic analysis based on 16S rRNA, gyrB , rpoB and atpD gene sequences supported the classification of strains 8N4 T , 9N2 and 10N3 within a novel species in a novel genus in the family Enterobacteriaceae . Strain 8N4 T , and the reference strains of the novel species, grew at 4–35 °C (optimum, 28–30 °C), with 0–5.0 % NaCl (optimum, 3 % NaCl) and with 0–60 % sucrose (optimum, 10–25 % sucrose). Their major cellular fatty acids were C 16 : 0 , C 17 : 0 cyclo, C 18 : 1 ω7 c and summed feature 3 (C 16 : 1 ω7 c and/or iso-C 15 : 0 2-OH). The DNA G+C content of strain 8N4 T was 46.8 mol%. On the basis of phenotypic properties and phylogenetic distinctiveness, the floral nectar isolates are classified within a novel species in a new genus in the family Enterobacteriaceae , for which the name Rosenbergiella nectarea gen. nov., sp. nov. is proposed. The type strain of Rosenbergiella nectarea is 8N4 T ( = LMG 26121 T = DSM 24150 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.052217-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2013
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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