In:
Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences, Vol. 94, No. 22 ( 1997-10-28), p. 11753-11757
Abstract:
The robust glow of molecular fluorescence renders even sparse
molecules detectable and susceptible to analysis for concentration, mobility, chemistry, and photophysics. Correlation spectroscopy, a
statistical-physics-based tool, gleans quantitative information from the spontaneously fluctuating fluorescence signals obtained from small
molecular ensembles. This analytical power is available for studying molecules present at minuscule concentrations in liquid solutions (less
than one nanomolar), or even on the surfaces of living cells at less than one macromolecule per square micrometer. Indeed, routines are
becoming common to detect, locate, and examine individual molecules under favorable conditions.
Type of Medium:
Online Resource
ISSN:
0027-8424
,
1091-6490
DOI:
10.1073/pnas.94.22.11753
Language:
English
Publisher:
Proceedings of the National Academy of Sciences
Publication Date:
1997
detail.hit.zdb_id:
209104-5
detail.hit.zdb_id:
1461794-8
SSG:
11
SSG:
12
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