In:
The Journal of Immunology, The American Association of Immunologists, Vol. 202, No. 1_Supplement ( 2019-05-01), p. 120.17-120.17
Abstract:
The bacterial microbiota correlated with the inflammatory response, and inflammations are developed with high frequency when infections happened in the upper respiratory tract (URT). However, it has rarely been studied that the mechanisms in the interaction between the bacterial microbiota and the infections appeared in URT. In our subjects, we researched the interaction between H1N1 infection and URT bacterial microbiota by IL-1 pathway in C57BL/6. We extracted the DNA in URT and performed 16S rRNA amplicon sequencing. When IL1 gene was knocked out, Streptococcus, Bergeyella and Enterococcus increased as major parts in the bacterial microbiota, and Staphylococcus, Rothia and Atopostipes decreased noticely. And then, the wild type mice and IL1−/− mice were infected by H1N1 in 1500TCID50, and the samples on Day1, Day3 and Day7 were collected to detect the URT bacterial microbiota. We found that these bacterias correlated with IL1 maybe play important roles in H1N1 infection. We validated the increase of streptococcus pneumoniae, Bergeyella zoohelcum and Enterococcus faecalis, and the decrease of Staphylococcus lentus and staphylococcus aureusby real time PCR. The RDA analysis and regression analysis showed that the virus load of H1N1 in URT correlated with the bacterial microbiota composition noticeably. The detection of the IgG, IgA and memory B cells showed that there were simmilar trends in wild type mice and IL1−/− mice, and the level of memory B cells in wild type mice were more. We analysized comprehensively the results above, and indicated that the clear correlation between the bacterias in URT and the H1N1 infection, maybe the URT bacterial microbiota could play roles in the immune system to response to the H1N1 infection.
Type of Medium:
Online Resource
ISSN:
0022-1767
,
1550-6606
DOI:
10.4049/jimmunol.202.Supp.120.17
Language:
English
Publisher:
The American Association of Immunologists
Publication Date:
2019
detail.hit.zdb_id:
1475085-5
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