In:
The Journal of Immunology, The American Association of Immunologists, Vol. 196, No. 1_Supplement ( 2016-05-01), p. 201.3-201.3
Abstract:
Respiratory Syncytia Virus (RSV) virus-like particles (VLPs) composed of of the two surface glycoproteins G, and F, and M protein self-assemble into VLPs. Both morphologically and immunologically, and are seen by the host cell as the real virus. RSV VLPs with adjuvant showed protection of the the lung and nose while RSV without adjuvant no protection in either site, while RSV without adjuvant provided to no protection in either site. To unravel the mystery behind the poor protection, we evaluated RSV VLPs-induced innate immunity and characterized RSV VLP-mediated cytokine response using human monocytic THP-1 cell line. Using PCR array we tested innate immune gene expression. We show that both the virus and the VLPs induced IFN-1β, pSTAT2 and interferon-stimulated genes (ISGs), namely ISG-15, ISG-56, IP-10 OAS-1 and MxA, suggesting the antiviral state in both cases. RSV-mediated induction of IFN-1β and ISG-15 seemed partially dependent on TLR-4, whereas RSV VLP-mediated induction of IFN-1β and the same ISGs was entirely dependent on TLR-4. Array data demonstrated that RSV signaled through MYD88 while RSV VLPs signaled through IRAK2, inducing NFKB activation. Furthermore, both RSV VLPs and RSV induced a Th1-biased cytokine response; however the RSV-induced responses were much weaker. Taken together, these findings suggest that RSV VLPs have several intrinsic properties that would make an effective and safe vaccine candidate when formulated with adjuvants.
Type of Medium:
Online Resource
ISSN:
0022-1767
,
1550-6606
DOI:
10.4049/jimmunol.196.Supp.201.3
Language:
English
Publisher:
The American Association of Immunologists
Publication Date:
2016
detail.hit.zdb_id:
1475085-5
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