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  • 1
    In: Cell, Elsevier BV, Vol. 185, No. 10 ( 2022-05), p. 1777-1792.e21
    Type of Medium: Online Resource
    ISSN: 0092-8674
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    Language: English
    Publisher: Elsevier BV
    Publication Date: 2022
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    detail.hit.zdb_id: 2001951-8
    SSG: 12
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  • 2
    In: American Journal of Respiratory and Critical Care Medicine, American Thoracic Society, Vol. 206, No. 12 ( 2022-12-15), p. 1534-1545
    Type of Medium: Online Resource
    ISSN: 1073-449X , 1535-4970
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    Language: English
    Publisher: American Thoracic Society
    Publication Date: 2022
    detail.hit.zdb_id: 1468352-0
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  • 3
    In: Gut, BMJ, Vol. 70, No. 11 ( 2021-11), p. 2123-2137
    Abstract: Microbiota disorder promotes chronic inflammation and carcinogenesis. High glycolysis is associated with poor prognosis in patients with colorectal cancer (CRC). However, the potential correlation between the gut microbiota and glucose metabolism is unknown in CRC. Design 18 F-FDG ( 18 F-fluorodeoxyglucose) PET (positron emission tomography)/CT image scanning data and microbiota PCR analysis were performed to measure the correlation between metabolic alterations and microbiota disorder in 33 patients with CRC. Multiple colorectal cancer models, metabolic analysis and Seahorse assay were established to assess the role of long non-coding RNA (lncRNA) enolase1-intronic transcript 1 (ENO1-IT1) in Fusobacterium (F.) nucleatum -induced glucose metabolism and colorectal carcinogenesis. RNA immunoprecipitation and chromatin immunoprecipitation sequencing were conducted to identify potential targets of lncRNA ENO1-IT1. Results We have found F . nucleatum abundance correlated with high glucose metabolism in patients with CRC. Furthermore, F. nucleatum supported carcinogenesis via increasing CRC cell glucose metabolism. Mechanistically, F. nucleatum activated lncRNA ENO1-IT1 transcription via upregulating the binding efficiency of transcription factor SP1 to the promoter region of lncRNA ENO1-IT1. Elevated ENO1-IT behaved as a guider modular for KAT7 histone acetyltransferase, specifying the histone modification pattern on its target genes, including ENO1, and consequently altering CRC biological function. Conclusion F. nucleatum and glucose metabolism are mechanistically, biologically and clinically connected to CRC. Targeting ENO1 pathway may be meaningful in treating patients with CRC with elevated F. nucleatum .
    Type of Medium: Online Resource
    ISSN: 0017-5749 , 1468-3288
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    Language: English
    Publisher: BMJ
    Publication Date: 2021
    detail.hit.zdb_id: 1492637-4
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  • 4
    In: Anesthesiology, Ovid Technologies (Wolters Kluwer Health), ( 2023-08-31)
    Abstract: Various studies have demonstrated racial disparities in perioperative care and outcomes. We hypothesize that among lower extremity total joint arthroplasty (TJA) patients, Evidence Based Perioperative Practice (EBPOP) utilization increased over time amongst all racial groups, and that standardized EBPOP care protocols resulted in reduction of racial disparities and improved outcomes. Methods We analyzed 3,356,805 lower extremity TJA patients from the Premier Healthcare database. The exposure of interest was race (White, Black, Asian, other). Outcomes were EBPOP adherence (8 individual care components; & gt;80% of these implemented was defined as ‘high EBPOP’), any major complication (including acute renal failure, delirium, myocardial infarction, pulmonary embolism, respiratory failure, stroke, or in-hospital mortality), in-hospital mortality, and prolonged length of stay (LOS). Results EBPOP adherence rate has increased over time and was associated with reduced complications across all racial groups. However, utilization amongst Black patients was below that for White patients between 2006 to 2021, (OR 0.94, 95% CI: 0.93, 0.95) (45.50% vs 47.90% on average). Independent of whether EBPOP components were applied, Black patients exhibited higher odds of major complications (1.61 (95% CI 1.55, 1.67) with high EBPOP; 1.43 (95% CI 1.39, 1.48) without high EBPOP), mortality (1.70 (95% CI 1.29, 2.25) with high EBPOP; 1.29 (95% CI 1.10, 1.51) without high EBPOP), and prolonged LOS (1.45 (95% CI 1.42, 1.48) with high EBPOP; 1.38 (95% CI 1.37, 1.40) without high EBPOP) compared to White patients. Conclusions EBPOP utilization in lower extremity joint arthroplasty has been increasing over the last decade. However racial disparities still exist with Black patients consistently having lower odds of EBPOP adherence. Black patients (compared to the White patients) exhibited higher odds of composite major complications, mortality, and prolonged LOS, independent of EBPOP use, suggesting that EBPOP did not impact racial disparities regarding especially Black patients in this surgical cohort.
    Type of Medium: Online Resource
    ISSN: 0003-3022 , 1528-1175
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    Language: English
    Publisher: Ovid Technologies (Wolters Kluwer Health)
    Publication Date: 2023
    detail.hit.zdb_id: 2016092-6
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  • 5
    Online Resource
    Online Resource
    Ovid Technologies (Wolters Kluwer Health) ; 2020
    In:  Anesthesia & Analgesia Vol. 131, No. 2 ( 2020-08), p. e99-e102
    In: Anesthesia & Analgesia, Ovid Technologies (Wolters Kluwer Health), Vol. 131, No. 2 ( 2020-08), p. e99-e102
    Type of Medium: Online Resource
    ISSN: 0003-2999
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    Language: English
    Publisher: Ovid Technologies (Wolters Kluwer Health)
    Publication Date: 2020
    detail.hit.zdb_id: 2018275-2
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  • 6
    In: Journal of Medical Virology, Wiley, Vol. 95, No. 3 ( 2023-03)
    Abstract: Epstein‐Barr virus (EBV)‐encoded Bam HI A rightward transcript (BART) microRNAs (miRNAs) play important roles in viral infection and tumorigenesis. The association of sequence variations in the BART miRNA cluster 1 region with diseases remains unclear. Herein, 6 types and 11 subtypes of BART cluster 1 were identified in 354 tumors and healthy donors (HDs) from nasopharyngeal carcinoma (NPC)‐endemic and nonendemic China (genotyped data), and 905 EBV genomes retrieved from GenBank from diseased and normal people from around the world (archived data). The distributions of BART cluster 1 types/subtypes between NPC‐endemic and nonendemic China; between Asian regions and Africa/Europe & Australia & United States; and among Asian regions (NPC‐endemic China, NPC‐nonendemic East Asia and Southeast Asia) were significantly different ( p   〈  0.001). The subtype BART‐D2 was not found outside Asia and was only common in NPC‐endemic China. More importantly, BART‐D2 had a higher frequency in NPCs than in HDs in NPC‐endemic China (genotyped data, 78.0% vs. 44.1%, p   〈  0.001; achieved data, 89.3% vs. 43.7%, p   〈  0.001), and was also more frequent in NPCs than in HDs, gastric carcinomas, and lymphomas in NPC‐nonendemic China (genotyped data, 27.9% vs. 1.9%, 2.4%, and 0.0%, p   〈  0.001). BART‐D2 was preferentially linked with the high‐risk subtypes for NPC previously reported, 162476C or 163364T, in the BALF2 gene, and was associated with NPC risk ( p   〈  0.01). In vitro experiments showed that BART‐D2 affected the expression of some mature BART miRNAs. These findings demonstrate geographically restricted variations of BART cluster 1 and identify distinct subtype that is confined to NPC‐endemic China and is associated with NPC.
    Type of Medium: Online Resource
    ISSN: 0146-6615 , 1096-9071
    URL: Issue
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    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 752392-0
    detail.hit.zdb_id: 1475090-9
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  • 7
    In: Oncogene, Springer Science and Business Media LLC, Vol. 39, No. 6 ( 2020-02-06), p. 1347-1360
    Abstract: Genome-wide association studies (GWASs) implicate 16q22.1 locus in risk for colorectal cancer (CRC). However, the underlying oncogenic mechanisms remain unknown. Here, through comprehensive filtration, we prioritized rs7198799, a common SNP in the second intron of the CDH1, as the putative causal variant. In addition, we found an association of CRC-risk allele C of rs7198799 with elevated transcript level of biological plausible candidate gene ZFP90 via expression quantitative trait loci analysis. Mechanistically, causal variant rs7198799 resides in an enhancer element and remotely regulate ZFP90 expression by targeting the transcription factor NFATC2. Remarkably, CRISPR/Cas9-guided single-nucleotide editing demonstrated the direct effect of rs7198799 on ZFP90 expression and CRC cellular malignant phenotype. Furthermore, ZFP90 affects several oncogenic pathways, including BMP4, and promotes carcinogenesis in patients and in animal models with ZFP90 specific genetic manipulation. Taken together, these findings reveal a risk SNP-mediated long-range regulation on the NFATC2-ZFP90-BMP4 pathway underlying the initiation of CRC.
    Type of Medium: Online Resource
    ISSN: 0950-9232 , 1476-5594
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    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2020
    detail.hit.zdb_id: 2008404-3
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  • 8
    Online Resource
    Online Resource
    Ovid Technologies (Wolters Kluwer Health) ; 2022
    In:  Anesthesia & Analgesia Vol. 134, No. 3 ( 2022-03), p. 540-547
    In: Anesthesia & Analgesia, Ovid Technologies (Wolters Kluwer Health), Vol. 134, No. 3 ( 2022-03), p. 540-547
    Abstract: The use of large data sources such as registries and claims-based data sets to perform health services research in anesthesia has increased considerably, ultimately informing clinical decisions, supporting evaluation of policy or intervention changes, and guiding further research. These observational data sources come with limitations that must be addressed to effectively examine all aspects of health care services and generate new individual- and population-level knowledge. Several statistical methods are growing in popularity to address these limitations, with the goal of mitigating confounding and other biases. In this article, we provide a brief overview of common statistical methods used in health services research when using observational data sources, guidance on their interpretation, and examples of how they have been applied to anesthesia-related health services research. Methods described involve regression, propensity scoring, instrumental variables, difference-in-differences, interrupted time series, and machine learning.
    Type of Medium: Online Resource
    ISSN: 0003-2999
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    Language: English
    Publisher: Ovid Technologies (Wolters Kluwer Health)
    Publication Date: 2022
    detail.hit.zdb_id: 2018275-2
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  • 9
    Online Resource
    Online Resource
    Ovid Technologies (Wolters Kluwer Health) ; 2022
    In:  Anesthesia & Analgesia Vol. 134, No. 3 ( 2022-03), p. 548-558
    In: Anesthesia & Analgesia, Ovid Technologies (Wolters Kluwer Health), Vol. 134, No. 3 ( 2022-03), p. 548-558
    Abstract: Anesthesia research using existing databases has drastically expanded over the last decade. The most commonly used data sources in multi-institutional observational research are administrative databases and clinical registries. These databases are powerful tools to address research questions that are difficult to answer with smaller samples or single-institution information. Given that observational database research has established itself as valuable field in anesthesiology, we systematically reviewed publications in 3 high-impact North American anesthesia journals in the past 5 years with the goal to characterize its scope. We identified a wide range of data sources used for anesthesia-related research. Research topics ranged widely spanning questions regarding optimal anesthesia type and analgesic protocols to outcomes and cost of care both on a national and a local level. Researchers should choose their data sources based on various factors such as the population encompassed by the database, ability of the data to adequately address the research question, budget, acceptable limitations, available data analytics resources, and pipeline of follow-up studies.
    Type of Medium: Online Resource
    ISSN: 0003-2999
    RVK:
    Language: English
    Publisher: Ovid Technologies (Wolters Kluwer Health)
    Publication Date: 2022
    detail.hit.zdb_id: 2018275-2
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  • 10
    Online Resource
    Online Resource
    Ovid Technologies (Wolters Kluwer Health) ; 2022
    In:  Circulation Research Vol. 131, No. Suppl_1 ( 2022-08-05)
    In: Circulation Research, Ovid Technologies (Wolters Kluwer Health), Vol. 131, No. Suppl_1 ( 2022-08-05)
    Abstract: Aims: Intravenous mesenchymal stem cells (MSCs) confer considerable cardiovascular benefits to patients. The lung, but not the heart, is the main trapped site for intravenous MSCs. It remains unclear how intravenous MSCs bridge this spatial gap between the lung and the heart to achieve cardiac repair. To investigate how intravenous MSCs, like an endocrine reservoir, secrete specific miRNA-enriched extracellular vesicles (EVs) gradually and continuously to alleviate doxorubicin (Dox)-induced cardiotoxicity. Methods and results: MSCs were isolated from human placenta tissue, and multiple molecular imaging was generated by modifying MSCs-DF or MSCs-Gluc transduced with firefly luciferase ( Fluc ) / enhanced green fluorescence protein (eGFP) or Gaussia luciferase (Gluc) lactadherin fusion protein. The Dox-induced cardiotoxicity murine model was constructed and an equal sum of MSCs was infused into mice by single high dose (5 х 10 6 MSCs) or repeated low dose (1 х 10 6 MSCs, 5 times) intravenously. Dox-induced cardiotoxicity, characterized by cardiac atrophy, left ventricular dysfunction, and injured myocardium, was alleviated by low doses of repeated MSCs. These cardioprotective effects were attributed to the suppression of GRP78 triggering stress-induced apoptosis of the endoplasmic reticulum (ER) in cardiomyocytes. Our results confirmed that miR-181a-5p in EVs derived from MSCs (MSC-EVs) inhibited GRP78. Intravenous MSCs-DF were trapped in the lung vasculature, secreted EVs into serum, which was confirmed by the detection of eGFP + EVs. Gluc activity increased in serum EVs from mice administrated with MSCs-Gluc. MiR-181a-5p, which inhibits GRP78 with high efficacy, expressed an increase in serum EVs and myocardium after injecting repeated low-dose MSCs. Up-regulation or down-regulation of miR-181a-5p levels in MSC-EVs had enhanced or weakened therapeutic effects on Dox-induced cardiotoxicity by modulating ER stress-induced apoptosis. Conclusion: This study identifies intravenous MSCs, recognized as an endocrine reservoir, secrete EVs enriched with miR-181a-5p in the blood to confer indirect cardioprotection against Dox-induced cardiotoxicity.
    Type of Medium: Online Resource
    ISSN: 0009-7330 , 1524-4571
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    Language: English
    Publisher: Ovid Technologies (Wolters Kluwer Health)
    Publication Date: 2022
    detail.hit.zdb_id: 1467838-X
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