In:
Journal of Leukocyte Biology, Oxford University Press (OUP), Vol. 55, No. 1 ( 1994-01-01), p. 127-132
Abstract:
Previously we have reported the differentiation-dependent expression of a soybean agglutinin (SBA)-binding structure on rat bone marrow cells (BMCs) during their differentiation into macrophages (møs). In the present study we tried to analyze the functional role of the SBA-binding structure in BMC proliferation and differentiation. Addition of SBA to BMC cultures driven into mø differentiation by recombinant murine granulocyte-macrophage colony-stimulating factor (rmGM-CSF), resulted in a two- to threefold increased proliferation rate compared with rmGM-CSF alone. However, the number of colonies in methyl cellulose was not increased by SBA. The effect of SBA was dose dependent (from 4 to 83 pM SBA), with a maximum effect at 83 pM. Experiments to detect a possible synergistic effect of additional cytokines produced by BMC after SBA treatment were inconclusive. The enhancing effect of SBA was also seen when high-density cells, which did not proliferate in response to rmGM-CSF (mainly granulocytes), were removed. Therefore, SBA may increase the CSF reactivity of re-sponsive mø progenitor cells directly by binding to N-acetylgalactosamine residues on their surface. J. Leukoc. Biol. 55: 127–132; 1994.
Type of Medium:
Online Resource
ISSN:
0741-5400
,
1938-3673
DOI:
10.1002/jlb.55.1.127
Language:
English
Publisher:
Oxford University Press (OUP)
Publication Date:
1994
detail.hit.zdb_id:
2026833-6
SSG:
12
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