In:
The Journal of Immunology, The American Association of Immunologists, Vol. 186, No. 1_Supplement ( 2011-04-01), p. 105.28-105.28
Abstract:
Cytomegalovirus is a clinically important pathogen. Previously we could show using murine cytomegalovirus (MCMV) that the adoptive transfer of memory B lymphocytes from immune donors into B- and T-cell immunodeficient RAG-/- mice completely protected from MCMV-induced morbidity and mortality. The protection is correlated with production of protective antibodies. Antibodies can act by neutralization of extracellular virus and/or antibody-dependent cellular cytotoxicity (ADCC) via Fc receptor-mediated effector functions. To investigate the role of Fc receptors for protection we adoptively transferred immune serum from infected donor mice into recipients that were deficient for the expression of Fcγ-receptors. First we used mice lacking the γ-chain (FcRγxRAG-/-), which is the accessory chain of the activating murine IgG receptors FcγRI, FcγRIII, and FcγRIV. FcRγxRAG-/- mice developed severe disease with high viral titers and had a significantly shorter survival compared to the RAG-/- control animals. Essentially no difference was seen between mice lacking the FcγRI and RAG-/- animals. Mice lacking the FcγRIII receptor were significantly less susceptible to MCMV infection than FcRγxRAG-/-, indicating that NK cells were not the major effector cell type involved, since FcγRIII is the only activating Fc-receptor on NK cells. Thus, although Fcγ-receptors are essential for antibody mediated protection from MCMV, NK cells seem not to be the crucial cell type involved.
Type of Medium:
Online Resource
ISSN:
0022-1767
,
1550-6606
DOI:
10.4049/jimmunol.186.Supp.105.28
Language:
English
Publisher:
The American Association of Immunologists
Publication Date:
2011
detail.hit.zdb_id:
1475085-5
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