In:
The Journal of the Acoustical Society of America, Acoustical Society of America (ASA), Vol. 105, No. 2_Supplement ( 1999-02-01), p. 1076-1076
Abstract:
There has been interest for a number of years in the possibility of separating, for transfusion proposes, blood cells from the suspending plasma in ultrasonic standing waves. On a much smaller scale, there has also been interest in separating cells and plasma so that the latter can be analyzed in single or multiple sample systems. Cells in whole blood occupy about 45% of the suspension volume. It has been shown with a number of different cell types, e.g., yeast and bacteria, that concentrations of this order are not as efficiently harvested by ultrasound as lower concentrations. Removal of cells from 2–5 ml whole blood volumes has been examined here in standing wave fields from plane or tubular transducers. The tubular transducers were driven at frequencies from 0.66 to 3.4 MHz. A range of frequencies, sonication pulsing regimes, and power levels have been established that result in cell separation efficiencies in excess of 99.5% in 5 min under conditions where cavitation has been avoided. The technique is now being applied to the blood of increasing numbers of volunteer donors to establish confidence in the ability to clarify plasma without cell damage. [Work supported by BBSRC and EU.]
Type of Medium:
Online Resource
ISSN:
0001-4966
,
1520-8524
Language:
English
Publisher:
Acoustical Society of America (ASA)
Publication Date:
1999
detail.hit.zdb_id:
1461063-2
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