In:
Pure and Applied Chemistry, Walter de Gruyter GmbH, Vol. 84, No. 2 ( 2012-01-10), p. 259-268
Abstract:
For the study, cabbage ( Brassica oleracea var. capitata L.) and red cabbage ( Brassica oleracea var. capitata L. f. rubra) were treated with Na selenate. Cabbage was foliarly sprayed twice with 20 mg Se(VI) L –1 , while red cabbage was fertilized twice with 0.5 mg Se(VI) L –1 . Despite the high dose of Se, no toxic effects were observed on cabbage plants. The total Se concentration in cabbage leaves was 4.80 ± 0.25 μg Se g –1 (DM) and in red cabbage 0.96 ± 0.04 μg Se g –1 (DM). Soluble Se compounds were extracted from parts of cabbage with protease XIV, resulting in 49 % of soluble Se from roots, 59 % from leaves, and 65 % from stems. In red cabbage, the corresponding figures were 28 % of soluble Se in roots, 31 % in stems, and 43 % in leaves. Se species were determined in the enzymatic extracts using ion exchange high-performance liquid chromatography-inductively coupled plasma-mass spectrometry (HPLC-ICP-MS). The main Se species found in all parts of cabbage and red cabbage was selenomethionine (SeMet), which in roots represented 94 and 55 % of the soluble Se content in cabbage and red cabbage, respectively. In stems and leaves of cabbage, SeMet represented only 23 % of the soluble Se content. In stems of red cabbage SeMet represented 80 % and in leaves 41 % of the soluble Se content. We observed that traces of Se(VI) were present in upper parts of both plants.
Type of Medium:
Online Resource
ISSN:
1365-3075
,
0033-4545
DOI:
10.1351/PAC-CON-11-07-19
Language:
Unknown
Publisher:
Walter de Gruyter GmbH
Publication Date:
2012
detail.hit.zdb_id:
2022101-0
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