In:
ChemBioChem, Wiley, Vol. 21, No. 4 ( 2020-02-17), p. 517-522
Abstract:
Spectroscopic characterization of Ag I ‐ion‐mediated C‐Ag I ‐A and C‐Ag I ‐T base pairs found in primer extension reactions catalyzed by DNA polymerases was conducted. UV melting experiments revealed that C‐A and C‐T mismatched base pairs in oligodeoxynucleotide duplexes are specifically stabilized by Ag I ions in 1:1 stoichiometry in the same manner as a C‐C mismatched base pair. Although the stability of the mismatched base pairs in the absence of Ag I ions is in the order C‐A≈C‐T 〉 C‐C, the stabilizing effect of Ag I ions follows the order C‐C 〉 C‐A≈C‐T. However, the comparative susceptibility of dNTPs to Ag I ‐mediated enzymatic incorporation into the site opposite templating C is dATP 〉 dTTP≫dCTP, as reported. The net charge, as well as the size and/or shape complementarity of the metal‐mediated base pairs, or the stabilities of mismatched base pairs in the absence of metal ions, would be more important than the stability of the metallo‐base pairs in the replicating reaction catalyzed by DNA polymerases.
Type of Medium:
Online Resource
ISSN:
1439-4227
,
1439-7633
DOI:
10.1002/cbic.201900450
Language:
English
Publisher:
Wiley
Publication Date:
2020
detail.hit.zdb_id:
2020469-3
SSG:
12
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