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  • Chemistry/Pharmacy  (6)
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  • Chemistry/Pharmacy  (6)
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  • 1
    Online Resource
    Online Resource
    Four‐Dimensional Deoxyribonucleic Acid–Gold Nanoparticle Assemblies
    Wiley ; 2020
    In:  Angewandte Chemie International Edition Vol. 59, No. 39 ( 2020-09-21), p. 17250-17255
    Details
    In: Angewandte Chemie International Edition, Wiley, Vol. 59, No. 39 ( 2020-09-21), p. 17250-17255
    Abstract: Organization of gold nanoobjects by oligonucleotides has resulted in many three‐dimensional colloidal assemblies with diverse size, shape, and complexity; nonetheless, autonomous and temporal control during formation remains challenging. In contrast, living systems temporally and spatially self‐regulate formation of functional structures by internally orchestrating assembly and disassembly kinetics of dissipative biomacromolecular networks. We present a novel approach for fabricating four‐dimensional gold nanostructures by adding an additional dimension: time. The dissipative character of our system is achieved using exonuclease III digestion of deoxyribonucleic acid (DNA) fuel as an energy‐dissipating pathway. Temporal control over amorphous clusters composed of spherical gold nanoparticles (AuNPs) and well‐defined core–satellite structures from gold nanorods (AuNRs) and AuNPs is demonstrated. Furthermore, the high specificity of DNA hybridization allowed us to demonstrate selective activation of the evolution of multiple architectures of higher complexity in a single mixture containing small and larger spherical AuNPs and AuNRs.
    Type of Medium: Online Resource
    ISSN: 1433-7851 , 1521-3773
    URL: Issue
    URL: Article
    DOI: 10.1002/anie.v59.39
    DOI: 10.1002/anie.202007616
    RVK:
    VA 2100
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 2011836-3
    detail.hit.zdb_id: 123227-7
    Location Call Number Limitation Availability
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    Online Resource
  • 2
    Online Resource
    Online Resource
    Aktivierung der katalytischen Aktivität von Thrombin für die Bildung von Fibrin durch Ultraschall
    Wiley ; 2021
    In:  Angewandte Chemie Vol. 133, No. 26 ( 2021-06-21), p. 14829-14836
    Details
    In: Angewandte Chemie, Wiley, Vol. 133, No. 26 ( 2021-06-21), p. 14829-14836
    Abstract: Die Regulierung der Aktivität von Enzymen ist eine Methode zur Steuerung biologischer Funktionen. Wir berichten über zwei Systeme, die die ultraschallinduzierte Aktivierung von Thrombin ermöglichen, das für die sekundäre Hämostase wichtig ist. Erstens entwickelten wir Polyaptamere, die spezifisch an Thrombin binden können und dessen katalytische Aktivität hemmen. Mit Hilfe von Ultraschall, der Trägheitskavitation erzeugt, und therapeutisch‐medizinischem fokussiertem Ultraschall lösen sich die Wechselwirkungen zwischen Polyaptamer und Enzym, wodurch die Aktivität zur Katalyse der Umwandlung von Fibrinogen in Fibrin wiederhergestellt wird. Zweitens verwendeten wir gespaltene Aptamere, die an die Oberfläche von Gold‐Nanopartikeln (AuNPs) konjugiert sind. In Gegenwart von Thrombin assemblieren diese zu einer Aptamer‐Tertiärstruktur, induzieren die Aggregation der AuNPs und deaktivieren das Enzym. Durch Behandlung mit Ultraschall lösen sich die AuNP‐Aggregate reversibel, setzen das Enzym frei und aktivieren es. Wir glauben, dass dieser Ansatz eine Blaupause für die Steuerung der Funktion anderer Proteine durch mechanische Stimuli im Bereich der Sonogenetik sein wird.
    Type of Medium: Online Resource
    ISSN: 0044-8249 , 1521-3757
    URL: Issue
    URL: Article
    DOI: 10.1002/ange.v133.26
    DOI: 10.1002/ange.202105404
    RVK:
    VA 2100
    RVK:
    VN 5020
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 505868-5
    detail.hit.zdb_id: 506609-8
    detail.hit.zdb_id: 514305-6
    detail.hit.zdb_id: 505872-7
    detail.hit.zdb_id: 1479266-7
    detail.hit.zdb_id: 505867-3
    detail.hit.zdb_id: 506259-7
    Location Call Number Limitation Availability
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    Online Resource
  • 3
    Online Resource
    Online Resource
    Activation of the Catalytic Activity of Thrombin for Fibrin Formation by Ultrasound
    Wiley ; 2021
    In:  Angewandte Chemie International Edition Vol. 60, No. 26 ( 2021-06-21), p. 14707-14714
    Details
    In: Angewandte Chemie International Edition, Wiley, Vol. 60, No. 26 ( 2021-06-21), p. 14707-14714
    Abstract: The regulation of enzyme activity is a method to control biological function. We report two systems enabling the ultrasound‐induced activation of thrombin, which is vital for secondary hemostasis. First, we designed polyaptamers, which can specifically bind to thrombin, inhibiting its catalytic activity. With ultrasound generating inertial cavitation and therapeutic medical focused ultrasound, the interactions between polyaptamer and enzyme are cleaved, restoring the activity to catalyze the conversion of fibrinogen into fibrin. Second, we used split aptamers conjugated to the surface of gold nanoparticles (AuNPs). In the presence of thrombin, these assemble into an aptamer tertiary structure, induce AuNP aggregation, and deactivate the enzyme. By ultrasonication, the AuNP aggregates reversibly disassemble releasing and activating the enzyme. We envision that this approach will be a blueprint to control the function of other proteins by mechanical stimuli in the sonogenetics field.
    Type of Medium: Online Resource
    ISSN: 1433-7851 , 1521-3773
    URL: Issue
    URL: Article
    DOI: 10.1002/anie.v60.26
    DOI: 10.1002/anie.202105404
    RVK:
    VA 2100
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 2011836-3
    detail.hit.zdb_id: 123227-7
    Location Call Number Limitation Availability
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    Online Resource
  • 4
    Online Resource
    Online Resource
    Four‐Dimensional Deoxyribonucleic Acid–Gold Nanoparticle Assemblies
    Wiley ; 2020
    In:  Angewandte Chemie Vol. 132, No. 39 ( 2020-09-21), p. 17403-17408
    Details
    In: Angewandte Chemie, Wiley, Vol. 132, No. 39 ( 2020-09-21), p. 17403-17408
    Abstract: Organization of gold nanoobjects by oligonucleotides has resulted in many three‐dimensional colloidal assemblies with diverse size, shape, and complexity; nonetheless, autonomous and temporal control during formation remains challenging. In contrast, living systems temporally and spatially self‐regulate formation of functional structures by internally orchestrating assembly and disassembly kinetics of dissipative biomacromolecular networks. We present a novel approach for fabricating four‐dimensional gold nanostructures by adding an additional dimension: time. The dissipative character of our system is achieved using exonuclease III digestion of deoxyribonucleic acid (DNA) fuel as an energy‐dissipating pathway. Temporal control over amorphous clusters composed of spherical gold nanoparticles (AuNPs) and well‐defined core–satellite structures from gold nanorods (AuNRs) and AuNPs is demonstrated. Furthermore, the high specificity of DNA hybridization allowed us to demonstrate selective activation of the evolution of multiple architectures of higher complexity in a single mixture containing small and larger spherical AuNPs and AuNRs.
    Type of Medium: Online Resource
    ISSN: 0044-8249 , 1521-3757
    URL: Issue
    URL: Article
    DOI: 10.1002/ange.v132.39
    DOI: 10.1002/ange.202007616
    RVK:
    VA 2100
    RVK:
    VN 5020
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 505868-5
    detail.hit.zdb_id: 506609-8
    detail.hit.zdb_id: 514305-6
    detail.hit.zdb_id: 505872-7
    detail.hit.zdb_id: 1479266-7
    detail.hit.zdb_id: 505867-3
    detail.hit.zdb_id: 506259-7
    Location Call Number Limitation Availability
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    Online Resource
  • 5
    Online Resource
    Online Resource
    Kontrolle über die optische und katalytische Aktivität gentechnisch hergestellter Proteine mit Ultraschall
    Wiley ; 2021
    In:  Angewandte Chemie Vol. 133, No. 3 ( 2021-01-18), p. 1515-1519
    Details
    In: Angewandte Chemie, Wiley, Vol. 133, No. 3 ( 2021-01-18), p. 1515-1519
    Abstract: Ultraschall (US) erzeugt mittels Kavitation mechanische Kräfte, die Polymerketten in Lösung dehnen und brechen. Diese Art der Polymer‐Mechanochemie wird für synthetische Polymere, nicht aber für Biomakromoleküle verwendet, obwohl US biokompatibel ist und gemeinhin für die medizinische Therapie sowie für die Bildgebung in vivo eingesetzt wird. Die Möglichkeit, die Aktivität von Proteinen durch US zu kontrollieren, wäre daher ein wichtiger Meilenstein für diese Disziplinen. Hier zeigen wir die ersten Beispiele für die selektive Aktivierung und Deaktivierung von Proteinen durch US. Mit der Verwendung von GFP als Modellsystem zeigen wir modellhaft, wie Proteine mit US‐Empfindlichkeit ausgestattet werden können. Der Einbau von langen und hochgeladenen Domänen ermöglicht eine effiziente Kraftübertragung auf die Proteinstruktur. Dieses Prinzip nutzen wir dann, um die katalytische Aktivität von Trypsin zu aktivieren, indem wir die Freisetzung seines Inhibitors auslösen. Wir erwarten, dass das Konzept des Ein‐ und Ausschaltens der Proteinaktivität durch US als Bauplan für die Fernsteuerung anderer bioaktiver Moleküle dienen wird.
    Type of Medium: Online Resource
    ISSN: 0044-8249 , 1521-3757
    URL: Issue
    URL: Article
    DOI: 10.1002/ange.v133.3
    DOI: 10.1002/ange.202010324
    RVK:
    VA 2100
    RVK:
    VN 5020
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 505868-5
    detail.hit.zdb_id: 506609-8
    detail.hit.zdb_id: 514305-6
    detail.hit.zdb_id: 505872-7
    detail.hit.zdb_id: 1479266-7
    detail.hit.zdb_id: 505867-3
    detail.hit.zdb_id: 506259-7
    Location Call Number Limitation Availability
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    Online Resource
  • 6
    Online Resource
    Online Resource
    Controlling Optical and Catalytic Activity of Genetically Engineered Proteins by Ultrasound
    Wiley ; 2021
    In:  Angewandte Chemie International Edition Vol. 60, No. 3 ( 2021-01-18), p. 1493-1497
    Details
    In: Angewandte Chemie International Edition, Wiley, Vol. 60, No. 3 ( 2021-01-18), p. 1493-1497
    Abstract: Ultrasound (US) produces cavitation‐induced mechanical forces stretching and breaking polymer chains in solution. This type of polymer mechanochemistry is widely used for synthetic polymers, but not biomacromolecules, even though US is biocompatible and commonly used for medical therapy as well as in vivo imaging. The ability to control protein activity by US would thus be a major stepping‐stone for these disciplines. Here, we provide the first examples of selective protein activation and deactivation by means of US. Using GFP as a model system, we engineer US sensitivity into proteins by design. The incorporation of long and highly charged domains enables the efficient transfer of force to the protein structure. We then use this principle to activate the catalytic activity of trypsin by inducing the release of its inhibitor. We expect that this concept to switch “on” and “off” protein activity by US will serve as a blueprint to remotely control other bioactive molecules.
    Type of Medium: Online Resource
    ISSN: 1433-7851 , 1521-3773
    URL: Issue
    URL: Article
    DOI: 10.1002/anie.v60.3
    DOI: 10.1002/anie.202010324
    RVK:
    VA 2100
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 2011836-3
    detail.hit.zdb_id: 123227-7
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
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