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  • 1
    Online Resource
    Online Resource
    The interaction of spermine with the ryanodine receptor from skeletal muscle
    Elsevier BV ; 1992
    In:  Biochimica et Biophysica Acta (BBA) - Biomembranes Vol. 1108, No. 1 ( 1992-07), p. 13-20
    Details
    In: Biochimica et Biophysica Acta (BBA) - Biomembranes, Elsevier BV, Vol. 1108, No. 1 ( 1992-07), p. 13-20
    Type of Medium: Online Resource
    ISSN: 0005-2736
    URL: Article
    DOI: 10.1016/0005-2736(92)90109-Y
    RVK:
    WA 15000
    Language: English
    Publisher: Elsevier BV
    Publication Date: 1992
    detail.hit.zdb_id: 2209384-9
    SSG: 12
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  • 2
    Online Resource
    Online Resource
    A hypothesis about the origin of carotenoid lipid droplets in the green algae Dunaliella and Haematococcus
    Springer Science and Business Media LLC ; 2019
    In:  Planta Vol. 249, No. 1 ( 2019-1), p. 31-47
    Details
    In: Planta, Springer Science and Business Media LLC, Vol. 249, No. 1 ( 2019-1), p. 31-47
    Type of Medium: Online Resource
    ISSN: 0032-0935 , 1432-2048
    URL: Article
    DOI: 10.1007/s00425-018-3050-3
    RVK:
    WA 15000
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2019
    detail.hit.zdb_id: 1463030-8
    SSG: 12
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  • 3
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    Characterization and photoaffinity labeling of the ATP binding site of the ryanodine receptor from skeletal muscle
    Wiley ; 1993
    In:  European Journal of Biochemistry Vol. 213, No. 1 ( 1993-04), p. 147-154
    Details
    In: European Journal of Biochemistry, Wiley, Vol. 213, No. 1 ( 1993-04), p. 147-154
    Type of Medium: Online Resource
    ISSN: 0014-2956 , 1432-1033
    URL: Issue
    URL: Article
    DOI: 10.1111/ejb.1993.213.issue-1
    DOI: 10.1111/j.1432-1033.1993.tb17744.x
    RVK:
    WA 15000
    Language: English
    Publisher: Wiley
    Publication Date: 1993
    detail.hit.zdb_id: 1398347-7
    detail.hit.zdb_id: 2172518-4
    SSG: 12
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  • 4
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    Cloning, molecular characterization, and phylogeny of two evolutionary distinct glutamine synthetase isoforms in the green microalga Haematococcus pluvialis (Chlorophyceae)
    Wiley ; 2016
    In:  Journal of Phycology Vol. 52, No. 6 ( 2016-12), p. 961-972
    Details
    In: Journal of Phycology, Wiley, Vol. 52, No. 6 ( 2016-12), p. 961-972
    Abstract: Haematococcus pluvialis (Chlorophyta) is a widely used microalga of great economic potential, yet its molecular genetics and evolution are largely unknown. We present new detailed molecular and phylogenetic analysis of two glutamine synthetase ( GS ) enzymes and genes ( gln ) under the Astaxanthin‐inducing conditions of light‐ and nitrogen‐stress. Structure analysis identified key residues and confirmed two decameric GS 2 holoenzymes, a cytoplasmic enzyme, termed GS 2c , and a plastidic form, termed GS 2p , due to chloroplast‐transit peptides at its N‐terminus. Gene expression analysis showed dissociation of mRNA , protein, and enzyme activity levels for both GS 2 under different growth conditions, indicating the strong post‐transcriptional regulation. Data‐mining identified novel and specified published gln genes from Prasinophyceae, Chlorophyta, Trebouxiophyceae, Charophyceae, Bryophyta, Lycopodiophyta, Spermatophyta, and Rhodophyta. Phylogenetic analysis found homologues to the cytosolic GS 2c of H. pluvialis in all other photo‐ and non‐photosynthetic Eukaryota. The chloroplastic GS 2p was restricted to Chlorophyta, Bryophyta, some Proteobacteria and Fungii; no homologues were identified in Spermatophyta or other Eukaryota. This indicates two independent prokaryotic donors for these two gln genes in H. pluvialis . Combined phylogenetic analysis of GS , chl‐ b synthase, elongation factor, and light harvesting complex homologues project a newly refined model of Viridiplantae evolution. Herein, a GS 1 evolved into the cytosolic GS 2c and was passed on to all Eukaryota. Later, the chloroplastic GS 2p entered the Archaeplastida lineage via a horizontal gene transfer at the divergence of Chlorophyta and Rhodophyta lineages. GS 2p persisted in Chlorophyta and Bryophyta, but was lost during Spermatophyta evolution. These data suggest the revision of GS classification and nomenclature, and extend our understanding of the photosynthetic Eukaryota evolution.
    Type of Medium: Online Resource
    ISSN: 0022-3646 , 1529-8817
    URL: Issue
    URL: Article
    DOI: 10.1111/jpy.2016.52.issue-6
    DOI: 10.1111/jpy.12444
    RVK:
    WA 15000
    Language: English
    Publisher: Wiley
    Publication Date: 2016
    detail.hit.zdb_id: 281226-5
    detail.hit.zdb_id: 1478748-9
    SSG: 12
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  • 5
    Online Resource
    Online Resource
    Does Astaxanthin Protect Haematococcus against Light Damage?
    Walter de Gruyter GmbH ; 1998
    In:  Zeitschrift für Naturforschung C Vol. 53, No. 1-2 ( 1998-2-1), p. 93-100
    Details
    In: Zeitschrift für Naturforschung C, Walter de Gruyter GmbH, Vol. 53, No. 1-2 ( 1998-2-1), p. 93-100
    Abstract: The photoprotective function of the ketocarotenoid astaxanthin in Haematococcus was questioned. When exposed to high irradiance and/or nutritional stress, green Haematococcus cells turned red due to accumulation of an immense quantity of the red pigment astaxanthin. Our results demonstrate that: 1) The addition of diphenylamine, an inhibitor of astaxanthin biosynthesis, causes cell death under high light intensity; 2) Red cells are susceptible to high light stress to the same extent or even higher then green ones upon exposure to a very high light intensity (4000 μmol photon m -2 s -1 ); 3) Addition of 1 O 2 generators (methylene blue, rose bengal) under noninductive conditions (low light of 100 (μmol photon m -2 s -1 ) induced astaxanthin accumulation. This can be reversed by an exogenous 1 O 2 quencher (histidine); 4) Histidine can prevent the accumulation of astaxanthin induced by phosphate starvation. We suggest that: 1) Astaxanthin is the result of the photoprotection process rather than the protective agent; 2) 1 O 2 is involved indirectly in astaxanthin accumulation process.
    Type of Medium: Online Resource
    ISSN: 1865-7125 , 0939-5075
    URL: Article
    DOI: 10.1515/znc-1998-1-217
    RVK:
    WA 15000
    Language: English
    Publisher: Walter de Gruyter GmbH
    Publication Date: 1998
    detail.hit.zdb_id: 2078107-6
    SSG: 12
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  • 6
    Online Resource
    Online Resource
    The Effect of the Herbicide Glufosinate (BASTA) on Astaxanthin Accumulation in the Green Alga Haematococcus pluvialis
    Walter de Gruyter GmbH ; 1999
    In:  Zeitschrift für Naturforschung C Vol. 54, No. 1-2 ( 1999-2-1), p. 49-54
    Details
    In: Zeitschrift für Naturforschung C, Walter de Gruyter GmbH, Vol. 54, No. 1-2 ( 1999-2-1), p. 49-54
    Abstract: The addition of 2.5 mᴍ glufosinate ammonium (BASTA), a well known plant killer, to Haematococcus pluvialis culture efficiently inhibits cell growth, blocks the activity of glutamine synthetase (GS) and induces astaxanthin accumulation. Conversely, methionine-S-sulfoximine (MSX), a well known GS inhibitor, had no effect on neither these parameters. When GS activity was tested in vitro, MSX inhibited the activity at high concentrations (mᴍ), while glufosinate was effective in the μm range. We have found that in the presence of glufosinate, ammonia is excreted from the cells. Therefore, we suggest that this process enables Haematococcus cells to escape the potentially harmful effect of glufosinate. As a consequence of the inability to assimilate nitrogen, astaxanthin is accumulated. This situation resembles the response of Haematococcus cells to nitrogen starvation
    Type of Medium: Online Resource
    ISSN: 1865-7125 , 0939-5075
    URL: Article
    DOI: 10.1515/znc-1999-1-209
    RVK:
    WA 15000
    Language: English
    Publisher: Walter de Gruyter GmbH
    Publication Date: 1999
    detail.hit.zdb_id: 2078107-6
    SSG: 12
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  • 7
    Online Resource
    Online Resource
    Patterns of carbohydrate and fatty acid changes under nitrogen starvation in the microalgae Haematococcus pluvialis and Nannochloropsis sp.
    Springer Science and Business Media LLC ; 2012
    In:  Applied Microbiology and Biotechnology Vol. 94, No. 6 ( 2012-6), p. 1495-1503
    Details
    In: Applied Microbiology and Biotechnology, Springer Science and Business Media LLC, Vol. 94, No. 6 ( 2012-6), p. 1495-1503
    Type of Medium: Online Resource
    ISSN: 0175-7598 , 1432-0614
    URL: Article
    DOI: 10.1007/s00253-012-3940-4
    RVK:
    WA 15000
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2012
    detail.hit.zdb_id: 1464336-4
    SSG: 12
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  • 8
    Online Resource
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    LIGHT‐INDUCED OIL GLOBULE MIGRATION IN HAEMATOCOCCUS PLUVIALIS (CHLOROPHYCEAE)
    Wiley ; 2012
    In:  Journal of Phycology Vol. 48, No. 5 ( 2012-10), p. 1209-1219
    Details
    In: Journal of Phycology, Wiley, Vol. 48, No. 5 ( 2012-10), p. 1209-1219
    Abstract: Astaxanthin‐rich oil globules in H aematococcus pluvialis display rapid light‐induced peripheral migration that is unique to this organism and serves to protect the photosynthetic system from excessive light. We observed rapid light‐induced peripheral migration that is associated with chlorophyll fluorescence quenching, whereas the recovery was slow. A simple assay to follow globule migration, based on chlorophyll fluorescence level has been developed. Globule migration was induced by high intensity blue light, but not by high intensity red light. The electron transport inhibitor dichlorophenyl‐dimethylurea did not inhibit globule migration, whereas the quinone analog (dibromo‐methyl‐isopropylbenzoquinone), induced globule migration even at low light. Actin microfilament‐directed toxins, such as cytochalasin B and latrunculin A , inhibited the light‐induced globule migration, whereas toxins against microtubules were ineffective. Electron microscopic ( EM ) imaging confirmed the cytoplasmic localization and peripheral migration of globules upon exposure to very high light ( VHL ). Scanning EM of freeze‐fractured cells also revealed globules within cytoplasmic bridges traversing the chloroplast, presumably representing the pathway of migration. Close alignments of globules with endoplasmic reticulum ( ER ) membranes were also observed following VHL illumination. We propose that light‐induced globule migration is regulated by the redox state of the photosynthetic electron transport system. Possible mechanisms of actin‐based globule migration are discussed.
    Type of Medium: Online Resource
    ISSN: 0022-3646 , 1529-8817
    URL: Issue
    URL: Article
    DOI: 10.1111/jpy.2012.48.issue-5
    DOI: 10.1111/j.1529-8817.2012.01210.x
    RVK:
    WA 15000
    Language: English
    Publisher: Wiley
    Publication Date: 2012
    detail.hit.zdb_id: 281226-5
    detail.hit.zdb_id: 1478748-9
    SSG: 12
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  • 9
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    INHIBITION OF ASTAXANTHIN SYNTHESIS UNDER HIGH IRRADIANCE DOES NOT ABOLISH TRIACYLGLYCEROL ACCUMULATION IN THE GREEN ALGA HAEMATOCOCCUS PLUVIALIS (CHLOROPHYCEAE) 1
    Wiley ; 2005
    In:  Journal of Phycology Vol. 41, No. 4 ( 2005-08), p. 819-826
    Details
    In: Journal of Phycology, Wiley, Vol. 41, No. 4 ( 2005-08), p. 819-826
    Abstract: Under stress conditions, Haematococcus pluvialis Flotow accumulates fatty acid–esterified astaxanthin, in extraplastidial lipid globules. The enhanced accumulation of fatty acids, mainly in triacylglycerols (TAG), among which oleic acid predominates, is linearly correlated with that of astaxanthin. We used inhibitors of either carotenoid or lipid biosynthesis to assess the interrelationship between carotenogenesis and TAG accumulation under high light irradiance as the stress factor. The two carotenogenesis inhibitors used—norflurazon, an inhibitor of phytoene desaturase, and diphenylamine (DPA), an inhibitor of β‐carotene C‐4 oxygenase—suppressed the accumulation of astaxanthin in a concentration‐dependent manner. Concurrently, the accumulation of neutral lipids was significantly less affected. The lipid biosynthesis inhibitor sethoxydim, which inhibits acetyl‐CoA carboxylase, significantly decreased de novo fatty acid synthesis and, in concert, drastically inhibited astaxanthin formation. In the presence of various concentrations of the three inhibitors, the inhibition of astaxanthin was not accompanied by a proportional decrease in oleic acid, which was used as a marker for TAG fatty acids. When astaxanthin synthesis was completely inhibited, the volumetric content of oleic acid was about 60% of the control value when the two carotenogenesis inhibitors (0.05 μM norflurazon or 20 μM DPA) were used and 27% of the control when the lipid‐synthesis inhibitor (50 μM) was used. We suggest therefore that TAG accumulation under high irradiance is not tightly coupled with astaxanthin accumulation, although the correlation between these two processes was demonstrated earlier. Furthermore, we propose that the accumulation of a certain amount of TAG is a prerequisite for the initiation of fatty acid–esterified astaxanthin accumulation in lipid globules.
    Type of Medium: Online Resource
    ISSN: 0022-3646 , 1529-8817
    URL: Issue
    URL: Article
    DOI: 10.1111/jpy.2005.41.issue-4
    DOI: 10.1111/j.0022-3646.2005.05015.x
    RVK:
    WA 15000
    Language: English
    Publisher: Wiley
    Publication Date: 2005
    detail.hit.zdb_id: 281226-5
    detail.hit.zdb_id: 1478748-9
    SSG: 12
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  • 10
    Online Resource
    Online Resource
    High Resolution Proteome of Lipid Droplets Isolated from the Pennate Diatom Phaeodactylum tricornutum ( Bacillariophyceae ) Strain pt4 provides mechanistic insights into complex intracellular coordination during nitrogen deprivation
    Wiley ; 2020
    In:  Journal of Phycology Vol. 56, No. 6 ( 2020-12), p. 1642-1663
    Details
    In: Journal of Phycology, Wiley, Vol. 56, No. 6 ( 2020-12), p. 1642-1663
    Abstract: Lipid droplets (LDs) are an organelle conserved amongst all eukaryotes, consisting of a neutral lipid core surrounded by a polar lipid monolayer. Many species of microalgae accumulate LDs in response to stress conditions, such as nitrogen starvation. Here, we report the isolation and proteomic profiling of LD proteins from the model oleaginous pennate diatom Phaeodactylum tricornutum , strain Pt4 (UTEX 646). We also provide a quantitative description of LD morphological ontogeny, and fatty acid content. Novel cell disruption and LD isolation methods, combined with suspension‐trapping and nanoflow liquid chromatography coupled to high resolution mass spectrometry, yielded an unprecedented number of LD proteins. Predictive annotation of the LD proteome suggests a broad assemblage of proteins with diverse functions, including lipid metabolism and vesicle trafficking, as well as ribosomal and proteasomal machinery. These proteins provide mechanistic insights into LD processes, and evidence for interactions between LDs and other organelles. We identify for the first time several key steps in diatom LD‐associated triacylglycerol biosynthesis. Bioinformatic analyses of the LD proteome suggests multiple protein targeting mechanisms, including amphipathic helices, post‐translational modifications, and translocation machinery. This work corroborates recent findings from other strains of P. tricornutum , other diatoms, and other eukaryotic organisms, suggesting that the fundamental proteins orchestrating LDs are conserved, and represent an ancient component of the eukaryotic endomembrane system. We postulate a comprehensive model of nitrogen starvation‐induced diatom LDs on a molecular scale, and provide a wealth of candidates for metabolic engineering, with the potential to eventually customize LD contents.
    Type of Medium: Online Resource
    ISSN: 0022-3646 , 1529-8817
    URL: Issue
    URL: Article
    DOI: 10.1111/jpy.v56.6
    DOI: 10.1111/jpy.13063
    RVK:
    WA 15000
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 281226-5
    detail.hit.zdb_id: 1478748-9
    SSG: 12
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