In:
European Journal of Biochemistry, Wiley, Vol. 244, No. 2 ( 1997-03), p. 494-500
Abstract:
Cosmid clones from –32 kb to +74 kb region of the rat somatotropin gene locus were isolated for examination of the chromatin structure in the region from –39kb to +47 kb by DNase I‐sensitivity analysis using rat pituitary‐derived GC (somatotropin+, prolactin–), and 235 (somatotropin–, prolactin+) cells, and liver‐derived BRL (somatotropin–, prolactin–) cells. DNase I‐hypersensitive sites (DHS) specific for somatotropin‐producing cells were previously shown to be located exclusively in the −2kb to +9kb region [ Aizawa, A., Yoneyama, T., Kazahari, K. & Ono, M. (1995) Nucleic Acids Res. 23 ,2236–2244] . No other DHS having this specificity was found in the region examined in this study. Except for these and two other DHS located in a cluster in this region, no DHS could be found from –23 kb to +22 kb. DHS having no or less cell‐type specificity were scattered about in the –39 kb to –23 kb and +22 kb to +47 kb regions. The polyadenylation site of the human skeletal‐muscle Na‐channel a‐subunit gene has been shown present 22 kb upstream from the somatotropin gene [ Bennani‐Baiti, I. M., Jones, B. K., Liebhaber, S. A. & Cooke, N. E. (1995) Genomics 29 ,647–652]. Polyadenylation site of the rat skeletal‐muscle Na‐channel gene was shown in this study to be at –15.7kb. The skeletal‐muscle Na‐channel gene was specifically expressed in skeletal‐muscle cells but not in somatotropin‐producing cells, and thus the boundary region that ensures the cell‐type‐specific expression of each gene would appear to be situated between two genes. The region prerequisite for cell‐type‐specific expression of the rat somatotropin gene was estimated based on the present findings.
Type of Medium:
Online Resource
ISSN:
0014-2956
,
1432-1033
DOI:
10.1111/ejb.1997.244.issue-2
DOI:
10.1111/j.1432-1033.1997.00494.x
Language:
English
Publisher:
Wiley
Publication Date:
1997
detail.hit.zdb_id:
1398347-7
detail.hit.zdb_id:
1464377-7
SSG:
12
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