In:
Physiologia Plantarum, Wiley, Vol. 123, No. 3 ( 2005-03), p. 331-338
Abstract:
The ripening inhibitor ( rin ) mutation of tomato yields non‐ripening fruit, and the gene corresponding to RIN , LeMADS‐RIN , is known to encode a transcriptional factor that controls ripening‐related genes. In this study, to evaluate the heterozygosity effect of rin on fruit ripening, we developed eight F 1 hybrid lines of the rin mutant from various crosses between the lines of the rin mutant and wild type. In the fruit of these F 1 hybrid lines, the shelf‐life was improved, but both the shelf‐life and colouring varied between the lines. We then chose one line of the F 1 hybrids and investigated the physiological and transcriptional properties of the fruit. Compared with the wild‐type parent, this F 1 line showed about half the lycopene content, lower fruit softening and lower mRNA accumulation of the genes that encode phytoene synthase ( Psy ), polygalacturonase ( PG ), β‐galactosidase ( TBG4 ) and expansin ( LeEXP1 ). The characteristic climacteric rise in ethylene production typically observed in the wild‐type parent during fruit maturation was not observed in the fruit of this F 1 line. The genes that encode ethylene biosynthetic enzymes, namely 1‐aminocyclopropane‐1‐carboxylic acid (ACC) synthase ( ACS2 and ACS4 ) and ACC oxidase ( ACO1 ), were, however, significantly expressed in the F 1 hybrid, suggesting that the post‐transcriptional regulator for activating these enzymes is affected by LeMADS‐RIN . These results suggest that the heterologous effect of LeMADS‐RIN in F 1 hybrids affects the gene transcription and activation of ripening‐related factors, resulting in changes in fruit properties, including the extension of the shelf‐life.
Type of Medium:
Online Resource
ISSN:
0031-9317
,
1399-3054
DOI:
10.1111/ppl.2005.123.issue-3
DOI:
10.1111/j.1399-3054.2005.00460.x
Language:
English
Publisher:
Wiley
Publication Date:
2005
detail.hit.zdb_id:
208872-1
detail.hit.zdb_id:
2020837-6
SSG:
12
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