GLORIA

GEOMAR Library Ocean Research Information Access

X

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Online Resource
    Online Resource
    Transmission Dynamics of Bartonella sp. Strain OE 1-1 in Sundevall's Jirds (Meriones crassus)
    American Society for Microbiology ; 2013
    In:  Applied and Environmental Microbiology Vol. 79, No. 4 ( 2013-02-15), p. 1258-1264
    Details
    In: Applied and Environmental Microbiology, American Society for Microbiology, Vol. 79, No. 4 ( 2013-02-15), p. 1258-1264
    Abstract: A high prevalence of Bartonella infection is found in many natural systems; however, the transmission dynamics leading to observations of these infections is not fully understood. The capability of Xenopsylla ramesis fleas to serve as competent vectors of Bartonella sp. OE 1-1 (a strain closely related to the zoonotic Bartonella elizabethae ) to Meriones crassus jirds was investigated. Naïve X. ramesis fleas were placed for 72 h on naïve jirds or jirds that were either experimentally or naturally infected with Bartonella sp. strain OE 1-1, after which they were placed on naïve jirds. Postfeeding, 69 to 100% of the fleas collected from each Bartonella -positive jird contained Bartonella DNA, and all naïve jirds became positive for Bartonella sp. OE 1-1 after infestation with the infected fleas. In addition, maternal transmission of Bartonella sp. OE 1-1 in jirds was tested by mating 5 Bartonella -positive and 5 naïve female jirds with 10 naïve male jirds in the absence of fleas. Fifteen offspring were delivered by each group. Cultures of blood drawn from all offspring on days 35 and 47 postdelivery were found to be negative for Bartonella . A single spleen sample from the offspring of a Bartonella -positive mother was found molecularly positive for Bartonella sp. OE 1-1. This study demonstrates that X. ramesis fleas are competent vectors of Bartonella sp. OE 1-1 to M. crassus jirds and indicates that maternal transmission is probably not the major transmission route from female jirds to their offspring. We suggest that the dynamics of Bartonella sp. OE 1-1 in the M. crassus jird population in nature is mostly dependent on its vectors.
    Type of Medium: Online Resource
    ISSN: 0099-2240 , 1098-5336
    URL: Article
    DOI: 10.1128/AEM.03011-12
    RVK:
    WA 15000
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 2013
    detail.hit.zdb_id: 223011-2
    detail.hit.zdb_id: 1478346-0
    SSG: 12
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 2
    Online Resource
    Online Resource
    Genomic structural plasticity of rodent‐associated Bartonella in nature
    Wiley ; 2022
    In:  Molecular Ecology Vol. 31, No. 14 ( 2022-07), p. 3784-3797
    Details
    In: Molecular Ecology, Wiley, Vol. 31, No. 14 ( 2022-07), p. 3784-3797
    Abstract: Rodent‐associated Bartonella species have shown a remarkable genetic diversity and pathogenic potential. To further explore the extent of the natural intraspecific genomic variation and its potential role as an evolutionary driver, we focused on a single genetically diverse Bartonella species, Bartonella krasnovii , which circulates among gerbils and their associated fleas. Twenty genomes from 16 different B. krasnovii genotypes were fully characterized through a genome sequencing assay (using short and long read sequencing), pulse field gel electrophoresis (PFGE), and PCR validation. Genomic analyses were performed in comparison to the B. krasnovii strain OE 1–1. While, single nucleotide polymorphism represented only a 0.3% of the genome variation, structural diversity was identified in these genomes, with an average of 51 ± 24 structural variation (SV) events per genome. Interestingly, a large proportion of the SVs ( 〉 40%) was associated with prophages. Further analyses revealed that most of the SVs, and prophage insertions were found at the chromosome replication termination site ( ter ), suggesting this site as a plastic zone of the B. krasnovii chromosome. Accordingly, six genomes were found to be unbalanced, and essential genes near the ter showed a shift between the leading and lagging strands, revealing the SV effect on these genomes. In summary, our findings demonstrate the extensive genomic diversity harbored by wild B. krasnovii strains and suggests that its diversification is initially promoted by structural changes, probably driven by phages. These events may constantly feed the system with novel genotypes that ultimately lead to inter‐ and intraspecies competition and adaptation.
    Type of Medium: Online Resource
    ISSN: 0962-1083 , 1365-294X
    URL: Issue
    URL: Article
    DOI: 10.1111/mec.v31.14
    DOI: 10.1111/mec.16547
    RVK:
    WA 15000
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 2020749-9
    detail.hit.zdb_id: 1126687-9
    SSG: 12
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 3
    Online Resource
    Online Resource
    Genetic characterization of spotted fever group rickettsiae in questing ixodid ticks collected in Israel and environmental risk factors for their infection
    Cambridge University Press (CUP) ; 2017
    In:  Parasitology Vol. 144, No. 8 ( 2017-07), p. 1088-1101
    Details
    In: Parasitology, Cambridge University Press (CUP), Vol. 144, No. 8 ( 2017-07), p. 1088-1101
    Abstract: This study aimed to genetically characterize spotted fever group rickettsiae (SFGR) in questing ixodid ticks from Israel and to identify risk factors associated with SFGR-positive ticks using molecular techniques and geographic information systems (GIS) analysis. 1039 ticks from the genus Rhipicephalus were collected during 2014. 109/1039 (10·49%) carried SFGR-DNA of either Rickettsia massiliae (95), ‘ Candidatus Rickettsia barbariae’ (8) or Rickettsia conorii (6). Higher prevalence of SFGR was found in Rhipicephalus turanicus (18·00%) compared with Rhipicephalus sanguineus sensu lato (3·22%). Rickettsia massiliae was the most commonly detected species and the most widely disseminated throughout Israel (87·15% of all Rickettsia -positive ticks). GIS analysis revealed that Central and Northern coastal regions are at high risk for SFGR. The presence of ticks was significantly associated with normalized difference vegetation index and temperature variation over the course of the year. The presence of rickettsiae was significantly associated with brown type soils, higher land surface temperature and higher precipitation. The latter parameters may contribute to infection of the tick with SFGR. Health care professionals should be aware of the possible exposure of local communities and travellers to R. massillae . Molecular and geographical information can help professionals to identify areas that are susceptible to SFGR-infected ticks.
    Type of Medium: Online Resource
    ISSN: 0031-1820 , 1469-8161
    URL: Article
    DOI: 10.1017/S0031182017000336
    RVK:
    WA 15000
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 2017
    detail.hit.zdb_id: 1491287-9
    SSG: 12
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 4
    Online Resource
    Online Resource
    Identification of Different Bartonella Species in the Cattle Tail Louse (Haematopinus quadripertusus) and in Cattle Blood
    American Society for Microbiology ; 2014
    In:  Applied and Environmental Microbiology Vol. 80, No. 17 ( 2014-09), p. 5477-5483
    Details
    In: Applied and Environmental Microbiology, American Society for Microbiology, Vol. 80, No. 17 ( 2014-09), p. 5477-5483
    Abstract: Bartonella spp. are worldwide-distributed facultative intracellular bacteria that exhibit an immense genomic diversity across mammal and arthropod hosts. The occurrence of cattle-associated Bartonella species was investigated in the cattle tail louse Haematopinus quadripertusus and in dairy cattle blood from Israel. Lice were collected from cattle from two dairy farms during summer 2011, and both lice and cow blood samples were collected from additional seven farms during the successive winter. The lice were identified morphologically and molecularly using 18S rRNA sequencing. Thereafter, they were screened for Bartonella DNA by conventional and real-time PCR assays using four partial genetic loci ( gltA , rpoB , ssrA , and internal transcribed spacer [ITS]). A potentially novel Bartonella variant, closely related to other ruminant bartonellae, was identified in 11 of 13 louse pools collected in summer. In the cattle blood, the prevalence of Bartonella infection was 38%, identified as B. bovis and B. henselae (24 and 12%, respectively). A third genotype, closely related to Bartonella melophagi and Bartonella chomelii (based on the ssrA gene) and to B. bovis (based on the ITS sequence) was identified in a single cow. The relatively high prevalence of these Bartonella species in cattle and the occurrence of phylogenetically diverse Bartonella variants in both cattle and their lice suggest the potential role of this animal system in the generation of Bartonella species diversity.
    Type of Medium: Online Resource
    ISSN: 0099-2240 , 1098-5336
    URL: Article
    DOI: 10.1128/AEM.01409-14
    RVK:
    WA 15000
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 2014
    detail.hit.zdb_id: 223011-2
    detail.hit.zdb_id: 1478346-0
    SSG: 12
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 5
    Online Resource
    Online Resource
    Use of Chimeric DNA-RNA Primers in Quantitative PCR for Detection of Ehrlichia canis and Babesia canis
    American Society for Microbiology ; 2009
    In:  Applied and Environmental Microbiology Vol. 75, No. 19 ( 2009-10), p. 6393-6398
    Details
    In: Applied and Environmental Microbiology, American Society for Microbiology, Vol. 75, No. 19 ( 2009-10), p. 6393-6398
    Abstract: To overcome the problem of nonspecific by-products in quantitative PCR (qPCR) assays, we constructed DNA-RNA chimeric primers and evaluated their use in the detection and quantification of the Ehrlichia canis 16S rRNA, Babesia canis Hsp70, and canine β-actin genes. Several RNA bases were incorporated into specific positions in the DNA primers, while no RNA stretches were allowed. qPCR reactions were carried out without preamplification steps. This resulted in decreased formation of undesirable by-products and a 10-fold increase in assay sensitivity.
    Type of Medium: Online Resource
    ISSN: 0099-2240 , 1098-5336
    URL: Article
    DOI: 10.1128/AEM.00720-09
    RVK:
    WA 15000
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 2009
    detail.hit.zdb_id: 223011-2
    detail.hit.zdb_id: 1478346-0
    SSG: 12
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 6
    Online Resource
    Online Resource
    Composition and Seasonal Variation of Rhipicephalus turanicus and Rhipicephalus sanguineus Bacterial Communities
    American Society for Microbiology ; 2012
    In:  Applied and Environmental Microbiology Vol. 78, No. 12 ( 2012-06-15), p. 4110-4116
    Details
    In: Applied and Environmental Microbiology, American Society for Microbiology, Vol. 78, No. 12 ( 2012-06-15), p. 4110-4116
    Abstract: A 16S rRNA gene approach, including 454 pyrosequencing and quantitative PCR (qPCR), was used to describe the bacterial community in Rhipicephalus turanicus and to evaluate the dynamics of key bacterial tenants of adult ticks during the active questing season. The bacterial community structure of Rh. turanicus was characterized by high dominance of Coxiella and Rickettsia and extremely low taxonomic diversity. Parallel diagnostic PCR further revealed a novel Coxiella species which was present and numerically dominant in all individual ticks tested ( n = 187). Coxiella sp. densities were significantly higher in female versus male ticks and were overall stable throughout the questing season. In addition, we revealed the presence of the novel Coxiella sp. in Rh. sanguineus adult ticks, eggs, and hatched larvae, indicating its vertical transmission. The presence of both spotted fever group Rickettsia spp. (SFGR) and non-SFGR was verified in the various individual ticks. The prevalence and density of Rickettsia spp. were very low compared to those of Coxiella sp. Furthermore, Rickettsia sp. densities were similar in males and females and significantly declined toward the end of the questing season. No correlation was found between Coxiella sp. and Rickettsia sp. densities. These results suggest different control mechanisms in the tick over its different bacterial populations and point to an obligatory and facultative association between the two tick species and Coxiella sp. and Rickettsia spp., respectively.
    Type of Medium: Online Resource
    ISSN: 0099-2240 , 1098-5336
    URL: Article
    DOI: 10.1128/AEM.00323-12
    RVK:
    WA 15000
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 2012
    detail.hit.zdb_id: 223011-2
    detail.hit.zdb_id: 1478346-0
    SSG: 12
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 7
    Online Resource
    Online Resource
    Vertical nontransovarial transmission of B artonella in fleas
    Wiley ; 2013
    In:  Molecular Ecology Vol. 22, No. 18 ( 2013-09), p. 4747-4752
    Details
    In: Molecular Ecology, Wiley, Vol. 22, No. 18 ( 2013-09), p. 4747-4752
    Abstract: Pathogens use diverse pathways to infect host populations by vertical and/or horizontal routes. Horizontal transmission of bacteria belonging to the B artonella genus via haematophagous vectors is well known. Vertical transmission of B artonella species was also suggested to occur but its routes remain to be unveiled. In a previous study, we showed the absence of transovarial transmission of B artonella species OE 1‐1 in X enopsylla ramesis fleas, and that fleas feeding on B artonella ‐positive jirds produced B artonella ‐positive gut voids. This current study aimed to investigate whether vertical nontransovarial transmission of B artonella occurs in fleas. For this aim, the X . ramesis– B artonella sp. OE 1‐1 model was used. Four groups of fleas including B artonella ‐positive and B artonella ‐negative female fleas and larval offspring had access to either B artonella ‐negative or B artonella ‐positive gut voids and faeces. Sixteen per cent of flea offspring that had access to B artonella ‐positive faeces and gut voids became B artonella positive. Our findings demonstrate that B artonella ‐positive flea faeces and gut voids are proper infection sources for flea larvae and indicate that vertical nontransovarial transmission of bartonellae occurs in fleas. This information broadens our understanding of B artonella transmission routes in flea vectors and enlightens pathways of bartonellae transmission and maintenance in flea populations in nature.
    Type of Medium: Online Resource
    ISSN: 0962-1083 , 1365-294X
    URL: Issue
    URL: Article
    DOI: 10.1111/mec.2013.22.issue-18
    DOI: 10.1111/mec.12408
    RVK:
    WA 15000
    Language: English
    Publisher: Wiley
    Publication Date: 2013
    detail.hit.zdb_id: 2020749-9
    detail.hit.zdb_id: 1126687-9
    SSG: 12
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 8
    Online Resource
    Online Resource
    Investigation of Bartonella acquisition and transmission in Xenopsylla ramesis fleas (Siphonaptera: Pulicidae) : BARTONELLA IN XENOPSYLLA RAMESIS FLEAS
    Wiley ; 2011
    In:  Molecular Ecology Vol. 20, No. 13 ( 2011-07), p. 2864-2870
    Details
    In: Molecular Ecology, Wiley, Vol. 20, No. 13 ( 2011-07), p. 2864-2870
    Type of Medium: Online Resource
    ISSN: 0962-1083
    URL: Article
    DOI: 10.1111/j.1365-294X.2011.05033.x
    RVK:
    WA 15000
    Language: English
    Publisher: Wiley
    Publication Date: 2011
    detail.hit.zdb_id: 2020749-9
    detail.hit.zdb_id: 1126687-9
    SSG: 12
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 9
    Online Resource
    Online Resource
    Detection of Bartonella spp. in wild carnivores, hyraxes, hedgehog and rodents from Israel
    Cambridge University Press (CUP) ; 2016
    In:  Parasitology Vol. 143, No. 10 ( 2016-09), p. 1232-1242
    Details
    In: Parasitology, Cambridge University Press (CUP), Vol. 143, No. 10 ( 2016-09), p. 1232-1242
    Abstract: Bartonella infection was explored in wild animals from Israel. Golden jackals ( Canis aureus ), red foxes ( Vulpes vulpes ), rock hyraxes ( Procavia capensis ), southern white-breasted hedgehogs ( Erinaceus concolor ), social voles ( Microtus socialis ), Tristram's jirds ( Meriones tristrami ), Cairo spiny mice ( Acomys cahirinus ), house mice ( Mus musculus ) and Indian crested porcupines ( Hystrix indica ) were sampled and screened by molecular and isolation methods. Bartonella -DNA was detected in 46 animals: 9/70 (13%) golden jackals, 2/11 (18%) red foxes, 3/35 (9%) rock hyraxes, 1/3 (33%) southern white-breasted hedgehogs, 5/57 (9%) Cairo spiny mice, 25/43 (58%) Tristram's jirds and 1/6 (16%) house mice. Bartonella rochalimae and B. rochalimae- like were widespread among jackals, foxes, hyraxes and jirds. This report represents the first detection of this zoonotic Bartonella sp. in rock hyraxes and golden jackals. Moreover, DNA of Bartonella vinsonii subsp. berkhoffii, Bartonella acomydis, Candidatus Bartonella merieuxii and other uncharacterized genotypes were identified. Three different Bartonella strains were isolated from Tristram's jirds, and several genotypes were molecularly detected from these animals. Furthermore, this study reports the first detection of Bartonella infection in a southern hedgehog. Our study indicates that infection with zoonotic and other Bartonella species is widespread among wild animals and stresses their potential threat to public health.
    Type of Medium: Online Resource
    ISSN: 0031-1820 , 1469-8161
    URL: Article
    DOI: 10.1017/S0031182016000603
    RVK:
    WA 15000
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 2016
    detail.hit.zdb_id: 1491287-9
    SSG: 12
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 10
    Online Resource
    Online Resource
    Bartonella Genotypes in Fleas (Insecta: Siphonaptera) Collected from Rodents in the Negev Desert, Israel
    American Society for Microbiology ; 2010
    In:  Applied and Environmental Microbiology Vol. 76, No. 20 ( 2010-10-15), p. 6864-6869
    Details
    In: Applied and Environmental Microbiology, American Society for Microbiology, Vol. 76, No. 20 ( 2010-10-15), p. 6864-6869
    Abstract: Fleas collected from rodents in the Negev Desert in southern Israel were molecularly screened for Bartonella species. A total of 1,148 fleas, collected from 122 rodents belonging to six species, were pooled in 245 pools based on flea species, sex, and rodent host species. Two Bartonella gene fragments, corresponding to RNA polymerase B ( rpoB ) and citrate synthase ( gltA ), were targeted, and 94 and 74 flea pools were found positive by PCR, respectively. The Bartonella 16S-23S internal transcribed spacer (ITS) region was also targeted, and 66 flea pools were found to be positive by PCR. Sixteen different Bartonella gltA genotypes were detected in 94 positive flea pools collected from 5 different rodent species, indicating that fleas collected from each rodent species can harbor several Bartonella genotypes. Based on gltA analysis, identified Bartonella genotypes were highly similar or identical to strains previously detected in rodent species from different parts of the world. A gltA fragment 100% similar to Bartonella henselae was detected in one flea pool. Another 2 flea pools contained gltA fragments that were closely related to B. henselae (98% similarity). The high sequence similarities to the zoonotic pathogen B. henselae warrant further investigation.
    Type of Medium: Online Resource
    ISSN: 0099-2240 , 1098-5336
    URL: Article
    DOI: 10.1128/AEM.00879-10
    RVK:
    WA 15000
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 2010
    detail.hit.zdb_id: 223011-2
    detail.hit.zdb_id: 1478346-0
    SSG: 12
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...