In:
FEBS Letters, Wiley, Vol. 590, No. 16 ( 2016-08), p. 2538-2548
Abstract:
Mycofactocin is a putative, peptide derived, cofactor that is associated primarily with the Mycobacterium genera including the pathogen M. tuberculosis . The pathway consists of the three genes mftA , mftB , and mftC that encode for the peptide substrate, peptide chaperone, and a radical S‐adenosylmethionine protein ( RS ), respectively. Here, we show that the MftB acts as a peptide chaperone, binding MftA with a submicromolar K D (~ 100 n m ) and MftC with a low micromolar K D (~ 2 μ m ). Moreover, we demonstrate that MftC is a radical S‐adenosylmethionine ( SAM ) enzyme. Finally, we show that MftC catalyzes the oxidative decarboxylation of the peptide MftA.
Type of Medium:
Online Resource
ISSN:
0014-5793
,
1873-3468
DOI:
10.1002/1873-3468.12249
Language:
English
Publisher:
Wiley
Publication Date:
2016
detail.hit.zdb_id:
1460391-3
SSG:
12
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