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  • 1
    Online Resource
    Online Resource
    Multi-omics analysis reveals the mechanism of bHLH130 responding to low-nitrogen stress of apple rootstock
    Oxford University Press (OUP) ; 2023
    In:  Plant Physiology Vol. 191, No. 2 ( 2023-02-12), p. 1305-1323
    Details
    In: Plant Physiology, Oxford University Press (OUP), Vol. 191, No. 2 ( 2023-02-12), p. 1305-1323
    Abstract: Nitrogen is critical for plant growth and development. With the increase of nitrogen fertilizer application, nitrogen use efficiency decreases, resulting in wasted resources. In apple (Malus domestica) rootstocks, the potential molecular mechanism for improving nitrogen uptake efficiency to alleviate low-nitrogen stress remains unclear. We utilized multi-omics approaches to investigate the mechanism of nitrogen uptake in two apple rootstocks with different responses to nitrogen stress, Malus hupehensis and Malus sieversii. Under low-nitrogen stress, Malus sieversii showed higher efficiency in nitrogen uptake. Multi-omics analysis revealed substantial differences in the expression of genes involved in flavonoid and lignin synthesis pathways between the two materials, which were related to the corresponding metabolites. We discovered that basic helix–loop–helix 130 (bHLH130) transcription factor was highly negatively associated with the flavonoid biosynthetic pathway. bHLH130 may directly bind to the chalcone synthase gene (CHS) promoter and inhibit its expression. Overexpressing CHS increased flavonoid accumulation and nitrogen uptake. Inhibiting bHLH130 increased flavonoid biosynthesis while decreasing lignin accumulation, thus improving nitrogen uptake efficiency. These findings revealed the molecular mechanism by which bHLH130 regulates flavonoid and lignin biosyntheses in apple rootstocks under low-nitrogen stress.
    Type of Medium: Online Resource
    ISSN: 0032-0889 , 1532-2548
    URL: Article
    DOI: 10.1093/plphys/kiac519
    RVK:
    WA 15000
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2023
    detail.hit.zdb_id: 2004346-6
    detail.hit.zdb_id: 208914-2
    SSG: 12
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  • 2
    Online Resource
    Online Resource
    Molecular cloning and characterization of a mannose-binding lectin gene from Crinum asiaticum
    Elsevier BV ; 2003
    In:  Journal of Plant Physiology Vol. 160, No. 8 ( 2003), p. 913-920
    Details
    In: Journal of Plant Physiology, Elsevier BV, Vol. 160, No. 8 ( 2003), p. 913-920
    Type of Medium: Online Resource
    ISSN: 0176-1617
    URL: Article
    DOI: 10.1078/0176-1617-01115
    RVK:
    WA 15000
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2003
    detail.hit.zdb_id: 2029184-X
    SSG: 12
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  • 3
    Online Resource
    Online Resource
    Nitrate transporter MdNRT2.4 interacts with rhizosphere bacteria to enhance nitrate uptake in apple rootstocks
    Oxford University Press (OUP) ; 2022
    In:  Journal of Experimental Botany Vol. 73, No. 18 ( 2022-10-18), p. 6490-6504
    Details
    In: Journal of Experimental Botany, Oxford University Press (OUP), Vol. 73, No. 18 ( 2022-10-18), p. 6490-6504
    Abstract: Plants have developed complex mechanisms to adapt to changing nitrate (NO3-) concentrations and can recruit microbes to boost nitrogen absorption. However, little is known about the relationship between functional genes and the rhizosphere microbiome in NO3- uptake of apple rootstocks. Here, we found that variation in Malus domestica NO3- transporter (MdNRT2.4) expression contributes to nitrate uptake divergence between two apple rootstocks. Overexpression of MdNRT2.4 in apple seedlings significantly improved tolerance to low nitrogen via increasing net NO3- influx at the root surface. However, inhibiting the root plasma membrane H+-ATPase activity abolished NO3- uptake and led to NO3- release, suggesting that MdNRT2.4 encodes an H+-coupled nitrate transporter. Surprisingly, the nitrogen concentration of MdNRT2.4-overexpressing apple seedlings in unsterilized nitrogen-poor soil was higher than that in sterilized nitrogen-poor soil. Using 16S ribosomal RNA gene profiling to characterize the rhizosphere microbiota, we found that MdNRT2.4-overexpressing apple seedlings recruited more bacterial taxa with nitrogen metabolic functions, especially Rhizobiaceae. We isolated a bacterial isolate ARR11 from the apple rhizosphere soil and identified it as Rhizobium. Inoculation with ARR11 improved apple seedling growth in nitrogen-poor soils, compared with uninoculated seedlings. Together, our results highlight the interaction of host plant genes with the rhizosphere microbiota for host plant nutrient uptake.
    Type of Medium: Online Resource
    ISSN: 0022-0957 , 1460-2431
    URL: Article
    DOI: 10.1093/jxb/erac301
    RVK:
    WA 15000
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2022
    detail.hit.zdb_id: 1466717-4
    SSG: 12
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  • 4
    Online Resource
    Online Resource
    At ROP 6 is involved in reactive oxygen species signaling in response to iron‐deficiency stress in Arabidopsis thaliana
    Wiley ; 2018
    In:  FEBS Letters Vol. 592, No. 20 ( 2018-10), p. 3446-3459
    Details
    In: FEBS Letters, Wiley, Vol. 592, No. 20 ( 2018-10), p. 3446-3459
    Abstract: Understanding the mechanism of iron (Fe)‐deficiency responses is crucial for improving plant Fe bioavailability. Here, we found that the Arabidopsis Rho‐like GTP ase 6 mutant ( rop6 ) is less sensitive to Fe‐deficiency responses and has reduced levels of reactive oxygen species ( ROS ) compared to wild‐type ( WT ), while At ROP 6 ‐overexpressing seedlings exhibit more sensitivity to Fe‐deficiency responses and has higher levels of ROS compared to WT . Moreover, treatment with H 2 O 2 improves the sensitivity to Fe‐deficiency responses in rop6 mutants. By using the yeast two‐hybrid system, we further demonstrate the direct interaction between At ROP 6 and Arabidopsis respiratory burst oxidase homolog D (At RBOHD ), which controls the generation of ROS . Overall, we suggest that At ROP 6 is involved in At RBOHD ‐mediated ROS signaling to modulate Fe‐deficiency responses in Arabidopsis thaliana .
    Type of Medium: Online Resource
    ISSN: 0014-5793 , 1873-3468
    URL: Issue
    URL: Article
    DOI: 10.1002/feb2.2018.592.issue-20
    DOI: 10.1002/1873-3468.13257
    RVK:
    WA 15000
    Language: English
    Publisher: Wiley
    Publication Date: 2018
    detail.hit.zdb_id: 1460391-3
    SSG: 12
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  • 5
    Online Resource
    Online Resource
    Engineering tropane biosynthetic pathway in Hyoscyamus niger hairy root cultures
    Proceedings of the National Academy of Sciences ; 2004
    In:  Proceedings of the National Academy of Sciences Vol. 101, No. 17 ( 2004-04-27), p. 6786-6791
    Details
    In: Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences, Vol. 101, No. 17 ( 2004-04-27), p. 6786-6791
    Abstract: Scopolamine is a pharmaceutically important tropane alkaloid extensively used as an anticholinergic agent. Here, we report the simultaneous introduction and overexpression of genes encoding the rate-limiting upstream enzyme putrescine N -methyltransferase (PMT) and the downstream enzyme hyoscyamine 6 β-hydroxylase (H6H) of scopolamine biosynthesis in transgenic henbane ( Hyoscyamus niger ) hairy root cultures. Transgenic hairy root lines expressing both pmt and h6h produced significantly higher ( P 〈 0.05) levels of scopolamine compared with the wild-type and transgenic lines harboring a single gene ( pmt or h6h ). The best line (T 3 ) produced 411 mg/liter scopolamine, which was over nine times more than that in the wild type (43 mg/liter) and more than twice the amount in the highest scopolamine-producing h6h single-gene transgenic line H 11 (184 mg/liter). To our knowledge, this is the highest scopolamine content achieved through genetic engineering of a plant. We conclude that transgenic plants harboring both pmt and h6h possessed an increased flux in the tropane alkaloid biosynthetic pathway that enhanced scopolamine yield, which was more efficient than plants harboring only one of the two genes. It seems that the pulling force of the downstream enzyme (the faucet enzyme) H6H plays a more important role in stimulating scopolamine accumulation in H. niger whereas the functioning of the upstream enzyme PMT is increased proportionally. This study provides an effective approach for large-scale commercial production of scopolamine by using hairy root culture systems as bioreactors.
    Type of Medium: Online Resource
    ISSN: 0027-8424 , 1091-6490
    URL: Article
    DOI: 10.1073/pnas.0401391101
    RVK:
    TA 1000
    RVK:
    WA 15000
    Language: English
    Publisher: Proceedings of the National Academy of Sciences
    Publication Date: 2004
    detail.hit.zdb_id: 209104-5
    detail.hit.zdb_id: 1461794-8
    SSG: 11
    SSG: 12
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