Description: Vazella pourtalesi presence and absence data were obtained from several different sources: DFO's multispecies trawl survey conducted in the Maritimes Region between 2007 to 2017 (presences and absences), DFO optical (in-house camera/video and remotely operated vehicle) benthic surveys conducted between 2001 and 2017 (presences only), and commercial bycatch records from the Fisheries Observer Program (FOP) from 1997 to 2007, and 2010 to 2015 (presences only). The DFO multispecies trawl survey is stratified random (by depth) and conducted using primarily Western IIA trawl gear. The average distance of these tows is ~ 3.17 km. Absence records were created from null (zero) catches that occurred in the same surveys. Commercial bycatch data between 1997 to 2007 from the Fisheries Observer Program was further post-processed and validated for accuracy, while the data from 2010 to 2015 was extracted directly from the Maritimes Fishery Science Database managed by DFO. Commercial trawls are much longer in duration and may follow bottom contours and/or retrace their footprint through the course of a 10+ km tow. For both the DFO multispecies trawl survey and FOP data, start coordinates were used to represent the tow, whereas for the DFO benthic imagery survey data the actual location of the V. pourtalesi record in situ was used. A 1 x 1 km grid matching that of the environmental data was placed over the study area and the presence-absence data were reduced to one record per cell, with a presence taking precedence over an absence if both occurred in the same cell. This gave a total of 215 presences (102 from the DFO trawl surveys, 47 from DFO benthic science surveys, and 66 from the FOP) and 2867 absences for the model.

Description: Ambient conditions shape microbiome responses to both short- and long-duration environment changes through processes including physiological acclimation, compositional shifts, and evolution. Thus, we predict that microbial communities inhabiting locations with larger diel, episodic, and annual variability in temperature and pH should be less sensitive to shifts in these climate-change factors. To test this hypothesis, we compared responses of surface ocean microbes from more variable (nearshore) and more constant (offshore) sites to short-term factorial warming (+3 °C) and/or acidification (pH -0.3). In all cases, warming alone significantly altered microbial community composition, while acidification had a minor influence. Compared with nearshore microbes, warmed offshore microbiomes exhibited larger changes in community composition, phylotype abundances, respiration rates, and metatranscriptomes, suggesting increased sensitivity of microbes from the less-variable environment. Moreover, while warming increased respiration rates, offshore metatranscriptomes yielded evidence of thermal stress responses in protein synthesis, heat shock proteins, and regulation. Future oceans with warmer waters may enhance overall metabolic and biogeochemical rates, but they will host altered microbial communities, especially in relatively thermally stable regions of the oceans.