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  • 1
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    d15N of lipids in marine animals of the Dutch Wadden Sea (2015)
    PANGAEA
    In:  Supplement to: Svensson, Elisabeth; Schouten, Stefan; Hopmans, Ellen C; Middelburg, Jack J; Sinninghe Damsté, Jaap S (2016): Factors Controlling the Stable Nitrogen Isotopic Composition (d15N) of Lipids in Marine Animals. PLoS ONE, 11(1), e0146321, https://doi.org/10.1371/journal.pone.0146321
    Details
    Publication Date: 2023-03-22
    Description: Lipid extraction of biomass prior to stable isotope analysis is known to cause variable changes in the stable nitrogen isotopic composition (d15N) of residual biomass. However, the underlying factors causing these changes are not yet clear. Here we address this issue by comparing the d15N of bulk and residual biomass of several marine animal tissues (fish, crab, cockle, oyster, and polychaete), as well as the d15N of the extracted lipids. As observed previously, lipid extraction led to a variable offset in d15N of biomass (differences ranging from -2.3 to +1.8 per mil). Importantly, the total lipid extract (TLE) was highly depleted in 15N compared to bulk biomass, and also highly variable (differences ranging from -14 to +0.7 per mil). The TLE consisted mainly of phosphatidylcholines, a group of lipids with one nitrogen atom in the headgroup. To elucidate the cause for the 15N-depletion in the TLE, the d15N of amino acids was determined, including serine because it is one of the main sources of nitrogen to N-containing lipids. Serine d15N values differed by -7 to +2 per mil from bulk biomass d15N, and correlated well with the 15N depletion in TLEs. On average, serine was less depleted (-3 per mil) than the TLE (-7 per mil), possibly due to fractionation during biosynthesis of N-containing headgroups, or that other nitrogen-containing compounds, such as urea and choline, or recycled nitrogen contribute to the nitrogen isotopic composition of the TLE. The depletion in 15N of the TLE relative to biomass increased with the trophic level of the organisms.
    Keywords: Carbon/Nitrogen ratio; Dutch_WaddenSea; Lipids; North Sea, Wadden sea; Sample code/label; Sample material; Species; Tissues; δ15N; δ15N, standard deviation
    Type: Dataset
    Format: text/tab-separated-values, 1367 data points
    Location Call Number Limitation Availability
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  • 2
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    Branched tetraether lipids in Carminowe Creek catchment (southwest England) (2020)
    PANGAEA
    Details
    Publication Date: 2023-01-16
    Description: In this dataset, the branched glycerol dialkyl glycerol tetraethers (brGDGTs) data in a creek catchment located in southwest England are included, with 78 soil samples along 14 soil transects from hilltop to downslope, including 31 arable land sites, 14 permanent grassland sites, 24 temporary grassland (ley) sites and 5 woodland sites, 7 riverbed sediments at three locations along each of the two tributaries (upstream, midstream and downstream), and one more at the joint outlet, and a 50 cm long sediment core in the lake, which is about 150 m away from the joint outlet. The soils or sediments were freeze dried and homogenized, and then they were extracted with dichloromethane (DCM):MeOH (9:1, v/v) with an accelerated solvent extractor to obtain the total lipid extracts (TLEs). After adding of a known amount of internal standard, the TLEs were dried under a nitrogen stream, and then separated into apolar and polar fractions by passing them over an activated aluminum oxide column (Al2O3). The polar fractions containing GDGTs were evaporated under a gentle nitrogen stream, and then the samples were re-dissolved in a hexane:isopropanol (99:1, v/v) and filtrated through a polytetrafluoroethylene (PTFE) filter for UHPLC analyzing.
    Keywords: Area/locality; Branched and isoprenoid tetraether index; Branched glycerol dialkyl glycerol tetraether, Ia, fractional abundance; Branched glycerol dialkyl glycerol tetraether, Ib, fractional abundance; Branched glycerol dialkyl glycerol tetraether, Ic, fractional abundance; Branched glycerol dialkyl glycerol tetraether, IIa, fractional abundance; Branched glycerol dialkyl glycerol tetraether, IIa', fractional abundance; Branched glycerol dialkyl glycerol tetraether, IIb, fractional abundance; Branched glycerol dialkyl glycerol tetraether, IIb', fractional abundance; Branched glycerol dialkyl glycerol tetraether, IIc, fractional abundance; Branched glycerol dialkyl glycerol tetraether, IIc', fractional abundance; Branched glycerol dialkyl glycerol tetraether, IIIa, fractional abundance; Branched glycerol dialkyl glycerol tetraether, IIIa', fractional abundance; Branched glycerol dialkyl glycerol tetraether, IIIb, fractional abundance; Branched glycerol dialkyl glycerol tetraether, IIIb', fractional abundance; Branched glycerol dialkyl glycerol tetraether, IIIc, fractional abundance; Branched glycerol dialkyl glycerol tetraether, IIIc', fractional abundance; Branched glycerol dialkyl glycerol tetraether, per unit mass total organic carbon; Calculated; Carbon, organic, total; Carminowe_Creek_catchment; Cornwall, United Kingdom; Crenarchaeol, fractional abundance; Crenarchaeol, per unit mass total organic carbon; Degree of cyclisation; Hexamethylated branched glycerol dialkyl glycerol tetraether IIIa/a'; Isomer ratio; Isoprenoid acyclic glycerol dialkyl glycerol tetraether, fractional abundance; Isoprenoid acyclic glycerol dialkyl glycerol tetraether, per unit mass total organic carbon; Isoprenoid acyclic glycerol dialkyl glycerol tetraether/Crenarchaeol ratio; LATITUDE; LONGITUDE; Methylation index of dominant branched tetraethers; Pentamethylated branched glycerol dialkyl glycerol tetraether IIa/a'; pH, soil; Sample ID; Sediment sample; see reference(s); SES; Sum branched glycerol dialkyl glycerol tetraether, per unit sediment mass; Tetramethylated branched glycerol dialkyl glycerol tetraether Ia; UHPLC; Ultra High Performance Liquid Chromatography
    Type: Dataset
    Format: text/tab-separated-values, 3417 data points
    Location Call Number Limitation Availability
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    DATA PANGAEA
  • 3
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    Nitrogen isotopes of bulk sediments from profile Wunstorf (2015)
    PANGAEA
    Details
    Publication Date: 2023-02-08
    Keywords: Calculated; Carbon, organic, total; Carbon, organic/Nitrogen, total ratio; DEPTH, sediment/rock; Nitrogen, total; Wunstorf; Wunstorf, Lower Saxony, Germany; δ15N
    Type: Dataset
    Format: text/tab-separated-values, 145 data points
    Location Call Number Limitation Availability
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  • 4
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    Comparison of the stable carbon and nitrogen isotopic values of gill and white muscle tissue of fish (2014)
    PANGAEA
    In:  Supplement to: Svensson, Elisabeth; Freitas, Vania; Schouten, Stefan; Middelburg, Jack J; van der Veer, H W; Sinninghe Damsté, Jaap S (2014): Comparison of the stable carbon and nitrogen isotopic values of gill and white muscle tissue of fish. Journal of Experimental Marine Biology and Ecology, 457, 173-179, https://doi.org/10.1016/j.jembe.2014.04.014
    Details
    Publication Date: 2023-02-12
    Description: The potential use of stable carbon and nitrogen isotope ratios (d13C, d15N) of fish gills for studies on fish feeding ecology was evaluated by comparing the d13C and d15N of gill tissue with the more commonly used white muscle tissue. To account for the effect of lipid content on the d13C signatures, a study-specific lipid correction model based on C:N ratios was developed and applied to the bulk d13C data. For the majority of species in the study, we found no significant difference in d13C values between gill and muscle tissue after correction, but several species showed a small (0.3-1.4 per mil) depletion in 13C in white muscle compared to gill tissue. The average species difference in d15N between muscle and gill tissue ranged from -0.2 to 1.6 per mil for the different fish species with muscle tissue generally more enriched in 15N. The d13C values of muscle and gill were strongly linearly correlated (R**2 = 0.85) over a large isotopic range (13 per mil), suggesting that both tissues can be used to determine long-term feeding or migratory habits of fish. Muscle and gill tissue bulk d15N values were also strongly positively correlated (R**2= 0.76) but with a small difference between muscle and gill tissue. This difference indicates that the bulk d15N of the two tissue types may be influenced by different isotopic turnover rates or a different composition of amino acids.
    Keywords: Carbon, organic, total; Carbon, organic, total, standard deviation; Carbon, organic/Nitrogen, total ratio; Comment; DATE/TIME; Length, total individual; Nitrogen, total; Nitrogen, total, standard deviation; Sample code/label; Sample type; Species; Texel_S; Wadden Sea; δ13C; δ13C, standard deviation; δ15N; δ15N, standard deviation
    Type: Dataset
    Format: text/tab-separated-values, 3490 data points
    Location Call Number Limitation Availability
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    DATA PANGAEA
  • 5
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    Results of an in situ stable isotope labeling study in intertidal sediment
    PANGAEA
    In:  Supplement to: van Oevelen, Dick; Moodley, Leon; Soetaert, Karline; Middelburg, Jack J (2006): The trophic significance of bacterial carbon in a marine intertidal sediment: Results of an in situ stable isotope labeling study. Limnology and Oceanography, 51(5), 2349-2359, https://doi.org/10.4319/lo.2006.51.5.2349
    Details
    Publication Date: 2023-05-12
    Description: We report the results of an in situ tracer experiment in an intertidal sediment, where bacterial carbon was tagged with stable carbon–isotope label, after the injection of 13C-glucose. The appearance of label in bacteria (based on label incorporation in bacteria-specific, phospholipid-derived fatty acids) and subsequent transfer to meiobenthos (group level) and macrobenthos (species level) was followed for 36 days. The label dynamics of benthic taxa were either fitted with a simple-isotope model or evaluated against enrichment in bacteria, to derive the importance of bacterially derived carbon for the meiobenthos and macrobenthos. Although selective uptake of bacteria was evident, as 2.4 times more bacterial carbon was grazed as expected from indiscriminate feeding, bacterial carbon accounted on average for only 0.08 and 0.11 of the carbon requirements of meiobenthic and macrobenthic taxa, respectively. Additionally, the contribution of bacterial carbon to total carbon requirements did not depend on the living/feeding depth in the sediment or organism size (evaluated over a size range of four orders of magnitude). The observed overall low contribution of bacterial carbon implies that most intertidal benthic fauna depend primarily on other carbon resources that may assert a stronger control on the structure of intertidal-sediment communities.
    Keywords: Benthos; Calculated; DATE/TIME; Depth, bottom/max; DEPTH, sediment/rock; Depth, top/min; Duration, number of days; EXP; Experiment; Molenplaat; Molenplaat intertidal mudflat; Scheldt Delta Estuary; Δδ13C
    Type: Dataset
    Format: text/tab-separated-values, 2142 data points
    Location Call Number Limitation Availability
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    DATA PANGAEA
  • 6
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    Phytoplankton-bacteria coupling under elevated CO2 levels: a stable isotope labelling study, 2010
    PANGAEA
    In:  Supplement to: de Kluijver, Anna; Soetaert, Karline; Schulz, Kai Georg; Riebesell, Ulf; Bellerby, Richard G J; Middelburg, Jack J (2010): Phytoplankton-bacteria coupling under elevated CO2 levels: a stable isotope labelling study. Biogeosciences, 7(11), 3783-3797, https://doi.org/10.5194/bg-7-3783-2010
    Details
    Publication Date: 2023-05-12
    Description: The potential impact of rising carbon dioxide (CO2) on carbon transfer from phytoplankton to bacteria was investigated during the 2005 PeECE III mesocosm study in Bergen, Norway. Sets of mesocosms, in which a phytoplankton bloom was induced by nutrient addition, were incubated under 1x (~350 µatm), 2x (~700 µatm), and 3x present day CO2 (~1050 µatm) initial seawater and sustained atmospheric CO2 levels for 3 weeks. 13C labelled bicarbonate was added to all mesocosms to follow the transfer of carbon from dissolved inorganic carbon (DIC) into phytoplankton and subsequently heterotrophic bacteria, and settling particles. Isotope ratios of polar-lipid-derived fatty acids (PLFA) were used to infer the biomass and production of phytoplankton and bacteria. Phytoplankton PLFA were enriched within one day after label addition, whilst it took another 3 days before bacteria showed substantial enrichment. Group-specific primary production measurements revealed that coccolithophores showed higher primary production than green algae and diatoms. Elevated CO2 had a significant positive effect on post-bloom biomass of green algae, diatoms, and bacteria. A simple model based on measured isotope ratios of phytoplankton and bacteria revealed that CO2 had no significant effect on the carbon transfer efficiency from phytoplankton to bacteria during the bloom. There was no indication of CO2 effects on enhanced settling based on isotope mixing models during the phytoplankton bloom, but this could not be determined in the post-bloom phase. Our results suggest that CO2effects are most pronounced in the post-bloom phase, under nutrient limitation.
    Keywords: Bacteria, biomass as carbon; Bacteria, delta-delta, weighted; Calculated, see reference(s); Carbon, inorganic, dissolved; Coccolithophoridae, biomass as carbon; Diatoms, biomass as carbon; EPOCA; European Project on Ocean Acidification; Experimental treatment; Experiment day; Green algae, biomass as carbon; Growth rate; Labelled bacteria in sediment traps relative to labelled bacteria in water column; Labelled phytoplankton in sediment traps relative to labelled phytoplankoton in water column; modelled; Phytoplankton, biomass as carbon; Phytoplankton, delta-delta, weighted
    Type: Dataset
    Format: text/tab-separated-values, 3090 data points
    Location Call Number Limitation Availability
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    DATA PANGAEA
  • 7
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    Hydrochemistry and process data measured at EUROTROPH station Randers_1 (2006)
    PANGAEA
    In:  Mécanique des Fluides Géophysiques, Université de Liège
    Details
    Publication Date: 2023-07-06
    Keywords: Carbon, organic, dissolved, flux; Carbon dioxide, air-sea, flux; Comment; CTD, Sea-Bird, SBE 9; DATE/TIME; DEPTH, water; Depth comment; EQL; Equilibrator; EUROTROPH; Gross primary production/Respiration rate ratio; Integrated gross primary production of oxygen; Integrated net community production of oxygen; Kattegat; LATITUDE; Limfjorden; LONGITUDE; Nutrients cycling and the trophic status of coastal ecosystems; Randers_1; Respiration rate, oxygen; Respiration rate, selenium; Salinity
    Type: Dataset
    Format: text/tab-separated-values, 57 data points
    Location Call Number Limitation Availability
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    DATA PANGAEA
  • 8
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    Hydrochemistry and process data measured at EUROTROPH station Randers_2 (2006)
    PANGAEA
    In:  Mécanique des Fluides Géophysiques, Université de Liège
    Details
    Publication Date: 2023-07-06
    Keywords: Carbon, organic, dissolved, flux; Comment; CTD, Sea-Bird, SBE 9; DATE/TIME; DEPTH, water; Depth comment; EQL; Equilibrator; EUROTROPH; Gross primary production/Respiration rate ratio; Integrated gross primary production of oxygen; Integrated net community production of oxygen; Kattegat; LATITUDE; Limfjorden; LONGITUDE; Nutrients cycling and the trophic status of coastal ecosystems; Randers_2; Respiration rate, oxygen; Respiration rate, selenium; Salinity
    Type: Dataset
    Format: text/tab-separated-values, 62 data points
    Location Call Number Limitation Availability
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    DATA PANGAEA
  • 9
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    Hydrochemistry and process data measured at EUROTROPH station Palma_1 (2006)
    PANGAEA
    In:  Mécanique des Fluides Géophysiques, Université de Liège
    Details
    Publication Date: 2023-07-06
    Keywords: Carbon, organic, dissolved, flux; Carbon dioxide, air-sea, flux; Comment; CTD, Sea-Bird, SBE 9; DATE/TIME; DEPTH, water; Depth comment; EQL; Equilibrator; EUROTROPH; Gross primary production/Respiration rate ratio; Integrated gross primary production of oxygen; Integrated net community production of oxygen; LATITUDE; LONGITUDE; Mediterranean Sea; Nutrients cycling and the trophic status of coastal ecosystems; Palma_1; Respiration rate, oxygen; Respiration rate, selenium; Salinity; SESAME; Southern European Seas: Assessing and Modelling Ecosystem Changes
    Type: Dataset
    Format: text/tab-separated-values, 63 data points
    Location Call Number Limitation Availability
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    DATA PANGAEA
  • 10
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    Nitrogen isotopes of bulk sediments of Hole 43-386 (2015)
    PANGAEA
    Details
    Publication Date: 2023-06-27
    Keywords: 43-386; Carbon, organic, total; Carbon, organic/Nitrogen, total ratio; Carbon, organic total/Phosphorus ratio; Deep Sea Drilling Project; DEPTH, sediment/rock; DRILL; Drilling/drill rig; DSDP; Glomar Challenger; Leg43; Nitrogen, total; North Atlantic/CONT RISE; Phosphorus; Sample code/label; δ13C, organic carbon; δ15N
    Type: Dataset
    Format: text/tab-separated-values, 128 data points
    Location Call Number Limitation Availability
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    DATA PANGAEA
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