Keywords:
Cell culture.
;
Electronic books.
Description / Table of Contents:
This reference guide is designed for industrial practitioners of mammalian cell cultivation for the production of biologics. This work is a culmination of two decades of accumulated expertise, practical know-how and best practices in cell culture technology. The Second Edition consists of major updates to all relevant topics.
Type of Medium:
Online Resource
Pages:
1 online resource (466 pages)
Edition:
2nd ed.
ISBN:
9780429531897
URL:
https://ebookcentral.proquest.com/lib/geomar/detail.action?docID=6129633
DDC:
616.0277
Language:
English
Note:
Cover -- Half Title -- Title Page -- Copyright Page -- Table of Contents -- Preface -- 1 Overview of Cell Culture Processes -- An Introduction to Cell Culture: A Historical Perspective -- Cell Culture as Process Technology -- Industrial Cell Lines -- Other Production Systems -- Manufacturing -- Upstream Process -- Product Quality in Cell Culture Processing -- Quality of the Product -- From Discovery to Clinical Products -- 2 Cell Biology for Bioprocessing -- Introduction -- Tissue Cells and Their Isolation -- Cell Membrane -- Cytoplasm and Organelles -- Protein Secretion through the ER and Golgi Apparatus -- Transport across Cellular Membranes -- Cell Shape, Mechanics, and Movement -- Growth Control -- Concluding Remarks -- 3 Cell Physiology -- Introduction -- Glucose and Energy Metabolism -- Regulation of Glucose Metabolism -- Transport and Transporters -- Metabolic Homeostasis and Lactate Consumption -- Amino Acid Metabolism -- Lipid Metabolism -- Glycan Biosynthesis and Protein Glycosylation -- Concluding Remarks -- 4 Product Quality and Process -- Introduction -- Quality of Protein Product -- Structural Homogeneity of the Product -- Biological Activities -- Quality by Design (QbD) of Cell Culture Products -- QTPP and CQAs -- Quality Risk Management -- Design Space and Control Strategy -- QbD in Different Stages of the Product's Life Cycle -- Concluding Remarks -- 5 Stoichiometry and the Kinetics of Cell Cultivation -- Introduction -- Material Conversion in Cell Culture -- Material Balance on Cell Growth -- Kinetic Description of Cell Growth & -- Product Formation -- Kinetic Model of Cell Growth -- Modeling Productivity -- Modeling a Cell Culture System -- Concluding Remarks -- 6 Cell Line Development -- Introduction -- Transient Production vs. Stable Expression Cell Line -- Host Cells.
,
Generating a Recombinant Protein High-Producing Cell Line -- Untitled -- Untitled -- Untitled.
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