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  • 1
    Online Resource
    Online Resource
    RADIOIMMUNOASSAY FOR ALDOSTERONE WITHOUT CHROMATOGRAPHY
    Oxford University Press (OUP) ; 1973
    In:  Acta Endocrinologica Vol. 74, No. 3 ( 1973-11), p. 548-557
    Details
    In: Acta Endocrinologica, Oxford University Press (OUP), Vol. 74, No. 3 ( 1973-11), p. 548-557
    Abstract: A radioimmunoassay for the determination of urinary aldosterone-18-glucuronide has been developed for the clinical laboratory. Ten ml of urine is pre-extracted with methylene chloride and then submitted to acid hydrolysis. This is followed by a second methylene chloride extraction. The aldosterone concentration in these extracts is determined with antisera obtained from rabbits immunized with an aldosterone-oxime bovine gamma globulin conjugate. In view of the high recovery of [1,2- 3 H] aldosterone no internal indicator standards were used. The sensitivity of the overall assay was 0.44 μg/24 h. The coefficient of variation was 3.7 % within a single assay and 13.6 % for multiple assays. The specificity of the method was examined by comparison with a double isotope dilution derivative method and by simultaneous use of 6 different antisera. Under normal sodium intake the urinary excretion of the acid-labile aldosterone-18-glucuronide ranged from 4.0 to 13.0 μg/24 h in 9 normal male subjects.
    Type of Medium: Online Resource
    ISSN: 0804-4643 , 1479-683X
    URL: Article
    DOI: 10.1530/acta.0.0740548
    RVK:
    WA 15000
    Language: Unknown
    Publisher: Oxford University Press (OUP)
    Publication Date: 1973
    detail.hit.zdb_id: 1485160-X
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  • 2
    Online Resource
    Online Resource
    RADIOIMMUNOASSAY FOR ALDOSTERONE WITHOUT CHROMATOGRAPHY
    Oxford University Press (OUP) ; 1973
    In:  Acta Endocrinologica Vol. 74, No. 3 ( 1973-11), p. 558-567
    Details
    In: Acta Endocrinologica, Oxford University Press (OUP), Vol. 74, No. 3 ( 1973-11), p. 558-567
    Abstract: A radioimmunoassay for the determination of plasma aldosterone has been developed suitable for the clinical laboratory. Two ml of plasma is extracted with methylene chloride and the aldosterone concentration is determined in these extracts with two different antisera obtained from rabbits immunized with an aldosterone-oxime bovine gamma globulin conjugate. In view of the high recovery of [1,2- 3 H] aldosterone no internal standards are needed. The coefficient of variation within a single assay was 9.0 and 10.6 % respectively. The coefficient of variation for inter-assay determinations was 13.5 and 15.0 % The validity of the method was tested by the determination of plasma aldosterone concentration in normal subjects during postural changes and salt restriction and in patients suffering from primary hyperaldosteronism. Both antisera gave almost identical values in 161 out of 170 plasma specimens. Since both antisera differed considerably in their degree of cross-reaction with various steroid hormones, the identical values obtained with the two antisera seem to provide a good indication that no cross-reaction with substances other than aldosterone had occurred in the assay systems.
    Type of Medium: Online Resource
    ISSN: 0804-4643 , 1479-683X
    URL: Article
    DOI: 10.1530/acta.0.0740558
    RVK:
    WA 15000
    Language: Unknown
    Publisher: Oxford University Press (OUP)
    Publication Date: 1973
    detail.hit.zdb_id: 1485160-X
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  • 3
    Online Resource
    Online Resource
    SUPPRESSION OF EPISODIC RENIN SECRETION IN NORMAL SUBSECTS BY PROPRANOLOL AND PINDOLOL
    Oxford University Press (OUP) ; 1975
    In:  Acta Endocrinologica Vol. 80, No. 1_Suppla ( 1975-12), p. S135-
    Details
    In: Acta Endocrinologica, Oxford University Press (OUP), Vol. 80, No. 1_Suppla ( 1975-12), p. S135-
    Type of Medium: Online Resource
    ISSN: 0804-4643 , 1479-683X
    URL: Article
    DOI: 10.1530/acta.0.080S135
    RVK:
    WA 15000
    Language: Unknown
    Publisher: Oxford University Press (OUP)
    Publication Date: 1975
    detail.hit.zdb_id: 1485160-X
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  • 4
    Online Resource
    Online Resource
    Measurement of 8-arginine-vasopressin by radioimmunoassay Development and application to urine and plasma samples using one extraction method
    Oxford University Press (OUP) ; 1984
    In:  Acta Endocrinologica Vol. 106, No. 3 ( 1984-07), p. 317-329
    Details
    In: Acta Endocrinologica, Oxford University Press (OUP), Vol. 106, No. 3 ( 1984-07), p. 317-329
    Abstract: Abstract. This paper describes the production and evaluation of an antiserum with high affinity (Ka = 1.9 × 10 11 l/mol) and specifity to 8-arginine-vasopressin (AVP). The antiserum binds only to the intact and unchanged ring and tail of the AVP-molecule. AVP was labelled with 125 I by a modification of the chloramin T method and purified by gel-filtration. We describe the development and validation of a radioimmunoassay for AVP in human plasma and urine, using only one extraction method (octa-decasilyl-treated silica microcolumns) for both biological fluids. The overall sensitivity of the assay method is 0.3 pg/ml plasma and 0.6 pg/ml urine. Mean (± sd ) plasma AVP-concentration in normally hydrated females was 1.2 ± 0.6 pg/ml (median 1.2 pg/ml) and 1.7 ± 0.7 pg/ml (median 1.8 pg/ml) in males. Mean urinary AVP excretion was 73 ± 43 ng/day with ad libitum water intake and normal activity. Sex differences were not statistically significant. We also assessed the response of plasma AVP and urinary AVP-excretion to waterload and dehydration.
    Type of Medium: Online Resource
    ISSN: 0804-4643 , 1479-683X
    URL: Article
    DOI: 10.1530/acta.0.1060317
    RVK:
    WA 15000
    Language: Unknown
    Publisher: Oxford University Press (OUP)
    Publication Date: 1984
    detail.hit.zdb_id: 1485160-X
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  • 5
    Online Resource
    Online Resource
    Lipoproteins Induce Expression of the Early Growth Response Gene-1 in Vascular Smooth Muscle Cells from Rat
    Elsevier BV ; 1993
    In:  Biochemical and Biophysical Research Communications Vol. 192, No. 2 ( 1993-04), p. 794-799
    Details
    In: Biochemical and Biophysical Research Communications, Elsevier BV, Vol. 192, No. 2 ( 1993-04), p. 794-799
    Type of Medium: Online Resource
    ISSN: 0006-291X
    URL: Article
    DOI: 10.1006/bbrc.1993.1484
    RVK:
    WA 15000
    Language: English
    Publisher: Elsevier BV
    Publication Date: 1993
    detail.hit.zdb_id: 1461396-7
    SSG: 12
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  • 6
    Online Resource
    Online Resource
    DIURNAL VARIATIONS OF PLASMA ALDOSTERONE IN SUPINE MAN: RELATIONSHIP TO PLASMA RENIN ACTIVITY AND PLASMA CORTISOL
    Oxford University Press (OUP) ; 1975
    In:  Acta Endocrinologica Vol. 80, No. 1 ( 1975-09), p. 95-103
    Details
    In: Acta Endocrinologica, Oxford University Press (OUP), Vol. 80, No. 1 ( 1975-09), p. 95-103
    Abstract: In order to investigate the role of renin secretion and of ACTH on the circadian rhythm of plasma aldosterone (PA), plasma renin activity (PRA), plasma cortisol (PC) and PA were determined at short-time intervals in 10 normal supine men. Six subjects were studied under a normal sodium intake and 4 under sodium restriction. In 4 subjects the secretion of ACTH was suppressed by dexamethasone. Under normal sodium intake changes in PA seemed to be more in parallel with changes in PC than by those in PRA as indicated by a higher significant correlation between PA and PC than between PA and PRA in 3 of the 4 subjects. In 1 subject no correlation was observed between PA and PC despite visual synchronism between the plasma concentrations of both hormones. Under dexamethasone medication fluctuations in PA were followed by those in PRA while PC was less than 2 μg/100 ml. In the sodium restricted state, changes in PA were closely paralleled and significantly correlated to PRA while no correlation was seen between PA and PC. Under dexamethasone medication the significant correlation between PA and PRA persisted. Our results indicate that in normal supine man the influence of ACTH and renin on PA may vary with different sodium intakes. Under normal sodium intake ACTH seems to be the dominant factor controlling PA, whereas under sodium restriction changes in PA are mediated through the renin angiotensin system. When the secretion of ACTH is suppressed by dexamethasone, renin controls PA both under normal and low sodium intake.
    Type of Medium: Online Resource
    ISSN: 0804-4643 , 1479-683X
    URL: Article
    DOI: 10.1530/acta.0.0800095
    RVK:
    WA 15000
    Language: Unknown
    Publisher: Oxford University Press (OUP)
    Publication Date: 1975
    detail.hit.zdb_id: 1485160-X
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  • 7
    Online Resource
    Online Resource
    PRIMARY ALDOSTERONISM: INABILITY TO DIFFERENTIATE UNILATERAL FROM BILATERAL ADRENAL LESIONS BY VARIOUS ROUTINE CLINICAL AND LABORATORY DATA AND BY PERIPHERAL PLASMA ALDOSTERONE
    Oxford University Press (OUP) ; 1978
    In:  Acta Endocrinologica Vol. 89, No. 4 ( 1978-12), p. 710-725
    Details
    In: Acta Endocrinologica, Oxford University Press (OUP), Vol. 89, No. 4 ( 1978-12), p. 710-725
    Abstract: In 31 patients with primary aldosteronism routine clinical and laboratory data, the effect of orthostasis on plasma aldosterone (PA), plasma renin activity (PRA) and cortisol (PC), effect of fludrocortisone or high sodium intake on basal PA and night-day fluctuations of basal PA and PC with and without suppression of pituitary ACTH by dexamethasone were determined to differentiate patients with a unilateral aldosterone producing tumour (adenoma, APA, n = 20; carcinoma, CA, n = 1) from those with idiopathic bilateral adrenal hyperplasia (IAH, n = 10). Mean systolic and diastolic blood pressure, age, serum sodium, serum potassium and urinary excretion of sodium and potassium were not significantly different in both groups of patients. Normokalaemic primary aldosteronism occurred both in patients with APA (n = 2) and in patients with IAH (n = 1). Mean basal PA and mean urinary excretion rate of aldosterone-18-glucuronide were higher though not significantly different in patients with APA or CA than in those with IAH. A substantial number of the patients with APA (n = 5) and with IAH (n = 3) showed urinary excretion rates of aldosterone-18-glucuronide ≦ 13 μg/24 h. Mean PA and PRA significantly increased ( P 〈 0.025) in patients with IAH in response to posture. However, these changes also occurred at times in some patients with APA. Both fludrocortisone and high sodium intake produced a variable and no group-specific effect on basal PA. Night-day variations in PA were positively correlated with those in PC in all patients with APA (n= 12) and in 5 of 8 patients with IAH. A dissociation of PA and PC, however, was only observed in patients with IAH. Finally, the effect of dexamethasone on plasma aldosterone curves was variable in both groups of patients. Our results indicate that under the described conditions analysis of routine clinical and laboratory data and of peripheral PA, PRA and PC are of limited value in differentiating patients with APA or CA from those with IAH.
    Type of Medium: Online Resource
    ISSN: 0804-4643 , 1479-683X
    URL: Article
    DOI: 10.1530/acta.0.0890710
    RVK:
    WA 15000
    Language: Unknown
    Publisher: Oxford University Press (OUP)
    Publication Date: 1978
    detail.hit.zdb_id: 1485160-X
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  • 8
    Online Resource
    Online Resource
    Sulfide-hemoglobin interactions in the sulfide-tolerant salt marsh resident, the California killifish Fundulus parvipinnis
    Springer Science and Business Media LLC ; 1992
    In:  Journal of Comparative Physiology B Vol. 162, No. 7 ( 1992-11), p. 614-624
    Details
    In: Journal of Comparative Physiology B, Springer Science and Business Media LLC, Vol. 162, No. 7 ( 1992-11), p. 614-624
    Type of Medium: Online Resource
    ISSN: 0174-1578 , 1432-136X
    URL: Article
    DOI: 10.1007/BF00296642
    RVK:
    WA 15000
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 1992
    detail.hit.zdb_id: 231245-1
    detail.hit.zdb_id: 1459302-6
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  • 9
    Online Resource
    Online Resource
    Thermoregulation in the wild boar (Sus scrofa)
    Springer Science and Business Media LLC ; 2023
    In:  Journal of Comparative Physiology B
    Details
    In: Journal of Comparative Physiology B, Springer Science and Business Media LLC
    Abstract: The wild boar ( Sus scrofa ) originates from warm islands but now inhabits large areas of the world, with Antarctica as the only continent not inhabited by this species. One might be tempted to think that its wide distribution results from increasing environmental temperatures. However, any effect of temperature is only indirect: Abundant availability of critical food resources can fully compensate the negative effects of cold winters on population growth. Here, we asked if temperature as a habitat factor is unimportant compared with other habitat indices, simply because wild boars are excellent thermoregulators. We found that the thermoneutral zone in summer was approximately 6–24 °C. In winter, the thermoneutral zone was lowered to 0–7 °C. The estimated increase in the heart rate and energy expenditure in the cold was less than 30% per 10 °C temperature decline. This relatively small increase of energy expenditure during cold exposure places the wild boar in the realm of arctic animals, such as the polar bear, whereas tropical mammals raise their energy expenditure several fold. The response of wild boars to high T a was weak across all seasons. In the heat, wild boars avoid close contact to conspecifics and particularly use wallowing in mud or other wet substrates to cool and prevent hyperthermia. Wild boars also rely on daily cycles, especially of rhythms in subcutaneous temperature that enables them to cheaply build large core–shell gradients, which serve to lower heat loss. We argue it is predominantly this ability which allowed wild boars to inhabit most climatically diverse areas in the world.
    Type of Medium: Online Resource
    ISSN: 0174-1578 , 1432-136X
    URL: Article
    DOI: 10.1007/s00360-023-01512-6
    RVK:
    WA 15000
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2023
    detail.hit.zdb_id: 231245-1
    detail.hit.zdb_id: 1459302-6
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  • 10
    Online Resource
    Online Resource
    On the origin of the branched alkyl substituents on ring B of flexirubin-type pigments
    Springer Science and Business Media LLC ; 1982
    In:  Archives of Microbiology Vol. 132, No. 3 ( 1982-9), p. 241-244
    Details
    In: Archives of Microbiology, Springer Science and Business Media LLC, Vol. 132, No. 3 ( 1982-9), p. 241-244
    Type of Medium: Online Resource
    ISSN: 0302-8933 , 1432-072X
    URL: Article
    DOI: 10.1007/BF00407958
    RVK:
    WA 15000
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 1982
    detail.hit.zdb_id: 1458451-7
    detail.hit.zdb_id: 477-7
    detail.hit.zdb_id: 124824-8
    SSG: 12
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