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  • Articles  (3)
  • Medicine  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Seasonal characteristics of the Indian Ocean Dipole during the Holocene epoch (2007)
    [s.l.] : Nature Publishing Group
    Nature 445 (2007), S. 299-302 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The Indian Ocean Dipole (IOD)—an oscillatory mode of coupled ocean–atmosphere variability—causes climatic extremes and socio-economic hardship throughout the tropical Indian Ocean region. There is much debate about how the IOD interacts with the El Niño/Southern ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nature05477
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    Paper (German National Licenses)
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  • 2
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    A novel, automated technology for multiplex biomarker imaging and application to breast cancer (2013)
    Wiley-Blackwell
    Details
    Publication Date: 2013-07-31
    Description: Aims Multiplexed immunofluorescence is a powerful tool for validating multi-gene assays and understanding the complex interplay of proteins implicated in breast cancer within a morphological context. We describe a novel technology for imaging an extended panel of biomarkers on a single, formalin-fixed paraffin embedded breast sample and evaluating biomarker interaction at a single cell level, and demonstrate proof-of-concept on a small set of breast tumours including those which co-express hormone receptors with Her2/neu and Ki-67. Methods and Results Using a microfluidic flow cell, reagent exchange was automated and consisted of serial rounds of staining with dye-conjugated antibodies, imaging, and chemical deactivation. A two-step antigen retrieval process was developed to satisfy all epitopes simultaneously, and key parameters were optimized. The imaging sequence was applied to seven breast tumours, comparing with conventional immunohistochemistry. Single-cell correlation analysis was performed with automated image processing. Conclusions We have described a novel platform for evaluating biomarker colocalization. Expression in multiplexed images is consistent with conventional IHC. Automation reduces inconsistencies in staining and positional shifts, while the fluorescent dye cycling approach dramatically expands the number of biomarkers which can be visualized and quantified on a single tissue section. This article is protected by copyright. All rights reserved.
    Print ISSN: 0309-0167
    Electronic ISSN: 1365-2559
    Topics: Medicine
    Published by Wiley-Blackwell
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  • 3
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    Tissue kallikrein protects SH-SY5Y neuronal cells against oxygen and glucose deprivation-induced injury through bradykinin B2 receptor-dependent regulation of autophagy induction (2016)
    Wiley-Blackwell
    Details
    Publication Date: 2016-06-02
    Description: Previous studies have demonstrated that tissue kallikrein (TK) protects against cerebral ischemia injury mainly through inhibition of apoptosis via bradykinin B2 receptor (B2R). In the present study, we proposed that autophagy induction contributed to the neuroprotective mechanism of TK. To validate this hypothesis, we investigated TK-induced autophagy and its signaling mechanisms in human SH-SY5Y cells exposed to oxygen and glucose deprivation (OGD). We found that TK treatment enhanced autophagy induction, reflected by augmented LC3 conversion and Beclin1 expression, decreased p62 levels and increased mRFP-LC3 puncta formation. GFP-mRFP-LC3 adenovirus assay indicated that TK maintained autophagic flux. Moreover, bafilomycin A1 (Baf.A1) caused obvious LC3-II accumulation either in the presence or absence of TK. Autophagy inhibition by Beclin1 knockdown or Baf.A1 treatment abrogated the neuroprotective effects of TK. MEK1/2/ERK1/2 and AMPK/TSC2/mTOR signaling were induced by OGD stress and enhanced by TK. MEK/ERK inhibitor U0126 alone elevated autophagy in OGD conditions but impaired TK-induced autophagy. Blockade of AMPK/TSC2/mTOR signaling by AMPK inhibitor compound C and shRNA mediated the knockdown of AMPK α1 and TSC2 but abolished autophagy in SH-SY5Y cells exposed to OGD treated either with or without TK. Moreover, B2R expression was upregulated by OGD exposure. B2R knockdown attenuated autophagy and suppressed MEK1/2/ERK1/2 and AMPK/TSC2/mTOR signaling in OGD conditions in either the presence or absence of TK. In sum, we revealed the significance of B2R-mediated MEK/ERK and AMPK signaling in autophagy induction under OGD stress, and proposed novel mechanisms involved in the neuropotective function of TK through B2R-dependent regulation of autophagy. This article is protected by copyright. All rights reserved.
    Print ISSN: 0022-3042
    Electronic ISSN: 1471-4159
    Topics: Medicine
    Published by Wiley-Blackwell on behalf of The International Society for Neurochemistry.
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