ISSN:
1573-5036
Keywords:
Bradyrhizobium
;
Chamaecytisus proliferus
;
subsp. palmensis
;
lipopolysaccharides
;
rhizobia natural population
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Abstract In the course of a study on rhizobia nodulating six indigenous legume shrubs from the Canary Islands, one Rhizobium and 27 Bradyrhizobium Canarian isolates were characterised. It was found that those ascribed to Bradyrhizobium were promiscuous and formed effective nodules not only in their original host but on Chamecytisus proliferus subsp. proliferus (Tagasaste) as well. However, Rhizobium isolate RES-1 was more specific and only nodulated on its host (Teline canariensis). The serotyping of these isolates required a broad antisera panel due to the great antigenic diversity of these rhizobia, that appeared to be due to differences in their lipopolysaccharides, the main antigenic determinants, that showed great structural diversity. The 28 isolates studied produced 22 easily distinguishable electrophoretic profiles of lipopolysaccharides. Protein or plasmid electrophoretic profiles were equally or less discriminating than the lipopolysaccharides profiles and were more difficult to compare. The comparison of the lipopolysaccharide electrophoretic patterns is a more reliable and discriminating method than serotyping or electrophoretic protein and plasmid profile analysis for the identification of Bradyrhizobium strains. No correlation between the lipopolysaccharide profiles of the isolates and the plant from which they were obtained or their geographical origin was observed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1004247803116
Permalink
