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  • 1
    ISSN: 1573-5117
    Keywords: primary production ; spectral light distribution ; incubator ; laboratory scale enclosure ; Oscillatoria limnetica ; Prochlorothrix hollandica
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In shallow, eutrophic Lake Loosdrecht, light attenuation and spectral changes with depth are pronounced due to high concentration of algae and tripton. Calculations of integrated daily primary production have been based on the photosynthesis-irradiance relationship (P/E-curve) obtained with the incubator technique. The suitability of this technique for estimating primary production in such a turbid lake was investigated by comparing P/E-curves for two differently pigmented algae, Oscillatoria limnetica and Prochlorothrix hollandica, in two laboratory systems differing in light regime. System (1) consisted of an incubator filled with tapwater, and system (2) was an algal culture in a laboratory scale enclosure (LSE). For O. limnetica the P/E-curves were identical. The P/E-curves for P. hollandica diverged considerably: the photosynthetic efficiency in the LSE (0.19 mg O2 (mg Chl)−1 h−1 (W m−2)−1 was about half of that obtained in the incubator (0.35 mg O2 (mg Chl)−1 h−1 (W m−2)−1. These results were explained by the interspecific variation in pigmentation. The photosynthetic efficiency of P. hollandica changed due to the dependence on the chlorophyll specific absorption for the whole photosynthetic waveband on the spectral light distribution, which for O. limnetica was approximately the same in the two light regimes. Column-integrated primary production computed with the photosynthetic characteristics of P. hollandica obtained in LSE, was 10–30% lower than the production based on incubator data.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-5117
    Keywords: laboratory scale enclosure ; underwater light field ; shallow lakes ; phytoplankton ; cyanobacteria ; spectral light distribution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Light conditions in laboratory scale enclosures (LSE) of shallow, eutrophic Lake Loosdrecht (The Netherlands), including a method for simulating a ‘natural’ incident light course, are described. Total PAR (400–700 nm) and spectral irradiance distribution were measured at sestonic chlorophyll a and dry weight concentrations ≥ 100 mg m−3 and 16 g m−3, respectively. Phytoplankton was dominated by Oscillatoria spp. The euphotic depth (Z eu) was 0.7–1.0 m. Shortly after filling the LSE with lake water, diffuse attenuation coefficients ranged from 14 m−1 for blue to 5 m−1 for red light. Around Z eu, attenuation in the blue region was markedly lower and irradiance reflectance (R) continued to increase; these anomalies were caused by lateral incident light from the LSE's waterbath. Spectrophotometry indicated that absorption was mainly by particles, but dissolved humic substances (gilvin) were also important. The particles were likely to be dominated by detritus absorbing more blue relative to red light. Subsurface R in lake water in the LSE had a maximum around 705 nm and low values in the blue band, but was lower than that previously reported for measurements in situ. Wash-out of detritus, presumably both dissolved and particulate fractions, by flow-through with synthetic medium, greatly affected the spectral reflectance measured outside the LSE. The maximum value of R decreased from 0.022 to 0.009, and the peak shifted to 550 nm.
    Type of Medium: Electronic Resource
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