ISSN:
1573-904X
Keywords:
xanthine oxidase inhibitors
;
flavonoids
;
proanthocyanidins
;
electrospray mass Spectrometry
;
Amyena scandens
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract Purpose. A previous screening showed that Amyena scandensDanser (Loranthaceae) efficiently inhibited XOD. The aim of this study was to identify the compounds with anti-XOD properties. For this purpose, Electrospray Tandem Mass Spectrometry (ESI-MS-MS) coupled with UV and Diode Array LC techniques were employed. Methods. Leaves were delipidized with petroleum ether and extracted with acetone:water 70:30, v:v. The extract was fractionated into the ethyl acetate and water soluble phases. Chemical investigation was performed following the bioactivity guided fractionation. Two fractions with anti-XOD activity were isolated by silica gel column chromatography of the ethyl acetate phase and analyzed by LC-UV-ESI-MS-MS. Results. The compounds identified with authentic standards were: catechin, epicatechin, epicatechin-3-gallate, quercetin-3-O-glucoside, quercetin-3-O-rhamnoside, and isorhamnetin-3-O-glucoside. Other constituents, only partially characterized, were a procyanidin dimer, a procyanidin trimer, three dimers epi/catechine-epi/catechine gallate and isorhamnetin-O-deoxyhexose. The anti-XOD activity was mainly due to galloyl-containing oligomeric proanthocyanidins. Conclusions. The coupling of UV Diode Array-HPLC with ESI-MS-MS represents a versatile tool for the rapid characterization of compounds in complex mixtures, avoiding time-consuming previous isolation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1011941002546
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