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  • 1
    ISSN: 1573-4927
    Keywords: protease inhibitor ; polymorphism ; genetics ; Monodelphis domestica
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Protease inhibitor (PI) polymorphism was observed in the laboratory opossum,Monodelphis domestica, by either one-dimensional acid polyacrylamide gel electrophoresis (PAGE; pH 4.6) or isoelectric focusing (pH 3.5-5.0) followed by immunoblotting with rabbit antiserum to human α1-antitrypsin; but acid PAGE produced superior resolution of the PI proteins. Family studies demonstrated an inheritance of nine codominant autosomal alleles,PI D ,PI E ,PI F ,PI G ,PI H ,PI I ,PI J ,PI K , andPI M , and a population study revealed frequencies of 0.411, 0.010, 0.341, 0.034, 0.023, 0.071, 0.035, 0.020, and 0.055, respectively.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Biochemical genetics 28 (1990), S. 495-501 
    ISSN: 1573-4927
    Keywords: baboon ; genetics ; isozyme ; mannose-6-phosphate isomerase ; polymorphism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Although erythrocytic mannose-6-phosphate isomerase (MPI) has been reported to be undetectable in mammals, we have demonstrated that sufficient activity is present in some species to enable its electrophoretic analysis. A survey of MPI from 2656 baboons revealed four allelic isozymes which segregated codominantly in pedigreed families. The gene frequencies differed significantly among five subspecies of baboons. However, theMPI*C allele had the highest frequency in all subspecies, ranging from 0.830 to 1.000. MPI phenotypes from erythrocytes and liver of the same individual were identical, indicating that the erythrocytic enzyme is specified by the same gene locus as the liver enzyme.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-4927
    Keywords: antithrombin III ; polymorphism ; genetics ; Monodelphis domestica
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Antithrombin III polymorphism was observed in the gray short-tailed opossum,Monodelphis domestica, by either one-dimensional polyacrylamide gel electrophoresis (PAGE; pH 7.9), two-dimensional PAGE (agarose, pH 5.4; 12% T, pH 7.9), or isoelectric focusing (pH 4.2–4.9) followed by immunoblotting with rabbit antiserum to human antithrombin III. Family studies demonstrated an inheritance of three codominant autosomal alleles, AT3A, AT3B, and AT3C, and a population study revealed frequencies of 0.70, 0.10, and 0.20, respectively.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-4927
    Keywords: complement factor ; polymorphism ; gene mapping ; linkage ; opossum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The sixth and seventh components of complement were found to be polymorphic and tightly linked in the laboratory opossum (Monodelphis domestica), as they are in eutherian mammals. In addition, strong evidence for linkage of theC6–C7 haplotype to the gene for glutamic pyruvate transaminase (GPT) was obtained for females but not for males. This result, combined with previous observations, establishes as a generality that recombination is severely reduced in females of this species by comparison with males. It also establishes synteny ofC6–C7 andGPT in a marsupial species, as exists in mice. Because these loci are not syntenic in humans, the results imply that this synteny is ancestral to the separation of marsupials and eutherians and that it was broken relatively recently in the mammalian lineage leading to human beings. The newly described C6 and C7 polymorphisms provide additional power for developing a linkage map for M. domestica and for localizing genes that confer susceptibility to diseases for which this species is used as a model.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1573-4927
    Keywords: antithrombin III ; polymorphism ; genetics ; Monodelphis domestica
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Antithrombin III polymorphism was observed in the gray short-tailed opossum,Monodelphis domestica, by either one-dimensional polyacrylamide gel electrophoresis (PAGE; pH 7.9), two-dimensional PAGE (agarose, pH 5.4; 12% T, pH 7.9), or isoelectric focusing (pH 4.2–4.9) followed by immunoblotting with rabbit antiserum to human antithrombin III. Family studies demonstrated an inheritance of three codominant autosomal alleles, AT3A, AT3B, and AT3C, and a population study revealed frequencies of 0.70, 0.10, and 0.20, respectively.
    Type of Medium: Electronic Resource
    Location Call Number Limitation Availability
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