ISSN:
1573-4943
Keywords:
Apolipoprotein H
;
β2-glycoprotein I
;
lectin affinity chromatography
;
glycosylation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract The specific binding of digoxigenin-labeled lectins to carbohydrate moieties is used to characterize the carbohydrate chains bound to apolipoprotein H. Our results show that apolipoprotein H is rich in sialic acid linked α(2–6) to galactose or N-acetylgalactosamine. Sialic acid is not α(2–3)-linked to galactose. Galactose is β(1–4)-linked to N-acetylglucosamine and β(1–3)-linked to N-acetylgalactosamine. High-mannose N-glycan chains are barely detectable. After N-glycosidase F treatment the molecular weight is substantially reduced. The main band is 32,500 daltons. Carbohydrate O-linked chains, which are mainly represented by sialic acid, are α(2–6)-linked to galactose or N-acetylgalactosamine. Galactose is also organized in O-linked chains and it is β(1–4)-linked to N-acetylglucosamine and β(1–3)-linked to acetylgalactosamine. Biochemical analysis of carbohydrate structures reveals that no specific carbohydrate complex is bound to a single isoform.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1026378825391
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