ISSN:
1573-0603
Keywords:
gut lymphocyte
;
Peyer's patch
;
blastogenic response
;
fecal contamination
;
local gut immunity
;
lymphoid organs
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary A procedure is described for culturing lymphocytes from Peyer's patches of the canine small intestine. A description is provided for the identification and removal of the lymphoid tissue (Peyer's patch), preparation of the tissue, separation of the lymphocytes from the tissue, and subsequent culturing of the lymphocytes. This procedure can be performed in 8 h, provides adequate antibiotic doses to prevent bacterial contamination, and describes how to remove excessive intestinal mucus from the preparation. This technique can be used for the preparation of lymphocytes from other lymphoid organs.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01665951
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