ISSN:
1573-5028
Keywords:
glutamine synthetase
;
Lupinus angustifolius
;
nitrogen fixation
;
nodule development
;
nodulin gene expression
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Glutamine synthetase, purified from Lupinus angustifolius legume nodules, was carboxymethylated and succinylated prior to chemical or enzymatic cleavage. Peptides were purified and sequenced. An oligonucleotide probe was constructed for the sequence MPGQW. This probe was used to identify a glutamine synthetase cDNA clone, pGS5, from a lupin nodule cDNA library constructed in pBR322. pGS5 was sequenced (1043 bp) and computer-assisted homology searching revealed a high degree of conservation between this lupin partial cDNA clone and other plant glutamine synthetases at both the amino acid (〉90%) and nucleotide (〉80%) level. Northern and Southern analyses using pGS5 supported the conclusion that a multigene glutamine synthetase family exists in lupin which is differentially expressed in both an organ-specific and temporal manner. Western and Northern blot analyses indicated the accumulation of a glutamine synthetase specific mRNA species during nodule development corresponded to the appearance of a novel glutamine synthetase polypeptide between 8 and 10 days after rhizobial inoculation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00027308
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